| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,414,454 | G→C | 16.4% | V246L (GTC→CTC) | acrE → | cytoplasmic membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,414,454 | 0 | G | C | 16.4% | 161.4 / 22.2 | 73 | V246L (GTC→CTC) | acrE | cytoplasmic membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (36/25); new base C (6/6); total (42/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAA > W3110S.gb/3414388‑3414522 | aGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCa < 1:2729248/70‑1 (MQ=255) ggCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATg > 1:3519754/1‑71 (MQ=255) gCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATg < 1:2962497/70‑1 (MQ=255) aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGaa < 1:3333571/69‑1 (MQ=255) aGCAATCCGTAGAGCAATGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAc > 1:72467/1‑71 (MQ=255) aGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAc < 1:3199247/71‑1 (MQ=255) aaTCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACgg < 1:2819700/70‑1 (MQ=255) tCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTc < 1:1559460/57‑1 (MQ=255) tCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCa < 1:725952/58‑1 (MQ=255) cGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTc > 1:818496/1‑68 (MQ=255) cGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTc > 1:1428814/1‑68 (MQ=255) gagCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCt < 1:2552198/70‑1 (MQ=255) agCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACg > 1:2254177/1‑60 (MQ=255) cAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATcc < 1:123167/71‑1 (MQ=255) aaaTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaa < 1:876456/58‑1 (MQ=255) aaTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGaa < 1:2317114/71‑1 (MQ=255) aTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTgg < 1:578012/39‑1 (MQ=255) tttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGt > 1:455240/1‑39 (MQ=255) tttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGt > 1:2358821/1‑39 (MQ=255) tttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGt > 1:317476/1‑39 (MQ=255) tttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATc > 1:3618233/1‑62 (MQ=255) ttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAg < 1:237479/70‑1 (MQ=255) tGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTAt > 1:227382/1‑59 (MQ=255) tGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAgg > 1:3509575/1‑70 (MQ=255) aTAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACg > 1:3386603/1‑71 (MQ=255) aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATg > 1:2119395/1‑36 (MQ=255) aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAc > 1:2299702/1‑42 (MQ=255) aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAc > 1:1238510/1‑42 (MQ=255) aGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAc > 1:156383/1‑41 (MQ=255) aGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTg > 1:664464/1‑71 (MQ=255) aGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTg > 1:1194845/1‑71 (MQ=255) ggAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaa < 1:337088/46‑1 (MQ=255) ggAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTg > 1:2869091/1‑70 (MQ=255) ggAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGc < 1:2413119/71‑1 (MQ=255) gAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGaa < 1:705086/59‑1 (MQ=255) cagagcgatgaaaacgtttcagtTTGCTCATGGAAAACgg > 1:2742474‑M2/24‑40 (MQ=255) cagagcgatgaaaacgtttcagtTTGCTCATGGAAAACGGt > 1:1162304‑M2/24‑41 (MQ=255) aaaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCaa < 1:1579364/70‑1 (MQ=255) aaaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCaa > 1:228905/1‑70 (MQ=255) aaaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCaa > 1:2822961/1‑70 (MQ=255) gagcgatgaaaacgtttcagtTTGCTCATGGAAAAc < 1:3196893‑M2/15‑1 (MQ=255) gagcgatgaaaacgtttcagtTTGCTCATGGAAAAc > 1:2121871‑M2/22‑36 (MQ=255) gagcgatgaaaacgtttcagtTTGCTCATGGAAAACg < 1:3017944‑M2/16‑1 (MQ=255) gagcgatgaaaacgtttcagtTTGCTCATGGAAAACGGt < 1:2610075‑M2/18‑1 (MQ=255) gagcgatgaaaacgtttcagtTTGCTCATGGAAAACGGt < 1:526245‑M2/18‑1 (MQ=255) aaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaaa > 1:1988910/1‑43 (MQ=255) aaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAAtt > 1:3603305/1‑71 (MQ=255) agcgatgaaaacgtttcagtTTGCTCATGGAAAACgg > 1:3011527‑M2/21‑37 (MQ=255) agcgatgaaaacgtttcagtTTGCTCATGGAAAACgg < 1:1658513‑M2/17‑1 (MQ=255) gcgatgaaaacgtttcagtTTGCTCATGGAAAACgg > 1:258721‑M2/20‑36 (MQ=255) gcgatgaaaacgtttcagtTTGCTCATGGAAAACg < 1:2562813‑M2/16‑1 (MQ=255) cGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaaa > 1:2125079/1‑41 (MQ=255) cGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAg > 1:920766/1‑56 (MQ=255) cGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct > 1:435959/1‑71 (MQ=255) cGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct > 1:894329/1‑71 (MQ=255) cgatgaaaacgtttcagtTTGCTCATGGAAAACGGt > 1:1173006‑M2/19‑36 (MQ=255) ccaccaGCAACGTAGAGTTTGTCATGGAAAACGGTCAAACCTATCCCCTGAAAgg < 1:127004/55‑1 (MQ=255) ccaccaGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTcc < 1:3159877/71‑1 (MQ=255) ccaGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGATGCAATTCTCCGAt > 1:1445869/1‑71 (MQ=255) caGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATccc > 1:2896589/1‑43 (MQ=255) caGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGa < 1:2235564/47‑1 (MQ=255) gCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTa > 1:1984759/1‑51 (MQ=255) gCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgt > 1:1419298/1‑70 (MQ=255) gCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgt > 1:2817602/1‑70 (MQ=255) cAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgtg < 1:2781750/70‑1 (MQ=255) aaCGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGAc < 1:3151206/71‑1 (MQ=255) gTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGaaa < 1:2143882/42‑1 (MQ=255) gTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGt < 1:745044/71‑1 (MQ=255) tAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGtt < 1:1576100/71‑1 (MQ=255) tAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGtt < 1:2442498/71‑1 (MQ=255) tAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGtt > 1:1995872/1‑71 (MQ=255) gagTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGa > 1:14305/1‑59 (MQ=255) gagTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtga > 1:939919/1‑71 (MQ=255) gagTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtga > 1:2767639/1‑71 (MQ=255) ggTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtgatga > 1:3474707/1‑69 (MQ=255) ggTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGATGCAATTCTCCGATGTGACCGTTGATGaa < 1:280554/70‑1 (MQ=255) | AGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAA > W3110S.gb/3414388‑3414522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |