| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,175 | A→G | 93.9% | R263R (CGA→CGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,175 | 0 | A | G | 93.9% | 1271.3 / 45.3 | 407 | R263R (CGA→CGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (12/13); new base G (194/187); total (206/201) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01 | |||||||||||
Only 100 of 408 total aligned reads displayed.
CTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > W3110S.gb/71108‑71242 | ccaCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa > 1:1642174/3‑71 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGa > 1:1749868/1‑65 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAttt < 1:354067/71‑1 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:2470111/1‑70 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:1175113/1‑70 (MQ=255) cGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa > 1:3340159/1‑70 (MQ=255) cGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa > 1:2165757/1‑70 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGTAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:858870/71‑1 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:3095193/71‑1 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGG‑TTTCACGATCAACTCTATTTCTCGCGGGTATTTaaa > 1:18433/1‑70 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:2924364/71‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:3561062/71‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:2353674/71‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:734910/71‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:3462633/71‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1070987/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaag < 1:2261499/71‑2 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:2008652/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:1862051/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:1196797/1‑70 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:1832495/66‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg < 1:2958749/70‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc < 1:923395/71‑1 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:1193234/1‑59 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAAt > 1:1988843/1‑67 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:2564347/1‑71 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:654956/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc > 1:480359/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc > 1:582954/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:2442559/1‑70 (MQ=255) cGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:2743254/61‑1 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:1874005/1‑61 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:643377/1‑61 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa > 1:842943/1‑59 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:1793248/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg < 1:2816219/70‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGTTATTTAAAAAATGCACCGGGGc < 1:449040/71‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:2052919/1‑71 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc < 1:784997/71‑1 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:536391/1‑57 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg > 1:3179374/1‑59 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCa > 1:3187573/1‑70 (MQ=255) gtcggtcgCAATGTTGGTTTTAACGATCAACTCTATTTCTCGCGGGTATTTaa > 1:1339056/1‑53 (MQ=39) tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGc > 1:1251397/1‑71 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGccc < 1:3133702/71‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGcc > 1:3667240/1‑70 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGccc > 1:3484772/1‑71 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg < 1:11492/60‑1 (MQ=255) cgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:2686663/49‑1 (MQ=255) cgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag > 1:1394939/1‑71 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:2867299/1‑71 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCctagc > 1:2901891/1‑70 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:3390733/71‑1 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAAt > 1:2936512/1‑49 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc < 1:1365203/52‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCggg > 1:427025/1‑55 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt < 1:360383/71‑1 (MQ=255) gTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGtt > 1:1483713/1‑71 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTTGCGGGTATTTAAAAAATGc < 1:3317465/47‑1 (MQ=39) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTa > 1:2308880/1‑35 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:1273360/1‑42 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:2322217/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:626966/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa > 1:1459464/1‑48 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:678377/1‑52 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcga > 1:1308722/1‑63 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcga > 1:2070529/1‑65 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCg > 1:1538189/1‑71 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCg > 1:1682149/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:2185400/1‑36 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATTCACCGGGGCCAGCCcgagcg > 1:1061764/1‑60 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc < 1:1802465/41‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:2788326/1‑43 (MQ=255) gACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCg < 1:3243077/70‑1 (MQ=255) aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCgg < 1:2503959/70‑1 (MQ=255) cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt > 1:3360494/1‑71 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCAGAGCGAGTTCCGTGCCGGTtgtg < 1:2412550/71‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:3226805/1‑35 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:2642306/1‑35 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGcc > 1:340761/1‑46 (MQ=255) cAACTCTATTTCTAGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:100058/1‑70 (MQ=255) ctctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaag > 1:89943/1‑70 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaag > 1:1917494/1‑68 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGccc < 1:2698212/41‑1 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:2981779/1‑70 (MQ=255) tATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa > 1:1620957/1‑70 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc < 1:2798621/49‑2 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:1931122/1‑68 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtg < 1:3673667/60‑1 (MQ=255) ttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt > 1:3066081/1‑71 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt > 1:3018719/1‑70 (MQ=255) ctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:2487365/70‑1 (MQ=255) ctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:1572970/70‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCCGCCCGAGCGAGTTCCGTGCCGGTGGTGAAGAAAAAGTGAat > 1:237288/1‑70 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa > 1:2209747/1‑64 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa > 1:3116233/1‑64 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa < 1:3586373/68‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2388727/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:1424809/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat > 1:1560502/1‑70 (MQ=255) | CTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > W3110S.gb/71108‑71242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |