| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,334,786 | Δ1 bp | 5.8% | coding (23/279 nt) | sfsB → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,334,786 | 0 | G | . | 5.8% | 217.5 / 4.4 | 69 | coding (23/279 nt) | sfsB | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (35/30); new base . (2/2); total (37/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAAT > W3110S.gb/3334717‑3334856 | gaagaaGTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTgg < 1:1946325/71‑1 (MQ=255) agTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATcc > 1:100943/1‑71 (MQ=255) agTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATcc > 1:902793/1‑71 (MQ=255) ttCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGc > 1:1304623/1‑70 (MQ=255) ttCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCt < 1:3192993/71‑1 (MQ=255) ttCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCt < 1:3249326/71‑1 (MQ=255) gaatgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAca > 1:1544893/1‑71 (MQ=255) gaatgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAca > 1:3619621/1‑71 (MQ=255) aatgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcat > 1:2589230/1‑71 (MQ=255) aatgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAc < 1:2589077/69‑1 (MQ=255) atgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatc < 1:387174/71‑1 (MQ=255) atgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatc < 1:2121511/71‑1 (MQ=255) tgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatca > 1:2670545/1‑71 (MQ=255) tgaaAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatca > 1:690223/1‑71 (MQ=255) aaaGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCAtt < 1:580289/71‑1 (MQ=255) gCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCg < 1:2893693/71‑1 (MQ=255) aCTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGc < 1:570807/67‑1 (MQ=255) aCTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGc < 1:589303/67‑1 (MQ=255) aCTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGt > 1:2150784/1‑71 (MQ=255) cTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCa > 1:1242694/1‑48 (MQ=255) aCTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTgc > 1:3668007/1‑71 (MQ=255) aCTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCAGCt > 1:1169562/1‑52 (MQ=255) aCTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACT‑GCATCCCGCTGACATCATTGCGGGttt < 1:3603679/68‑1 (MQ=255) aCTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACT‑GCATCCCGCTGACATCATTGCGGGttt < 1:3358779/68‑1 (MQ=255) aCTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACT‑GCATCCCGCTGACATCATTGCGGGTTTgc > 1:1590824/1‑70 (MQ=255) aCTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACT‑GCATCCCGCTGACATCATTGCGGGTTTgc > 1:3677638/1‑70 (MQ=255) aaGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCga > 1:1480275/1‑46 (MQ=255) aaGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTgcgc > 1:3567277/1‑69 (MQ=255) aaGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTgcgc > 1:2513810/1‑69 (MQ=255) aaGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTgcgc > 1:2590504/1‑69 (MQ=255) aGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTgcgc > 1:3419341/1‑68 (MQ=255) aGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:3787783/71‑1 (MQ=255) aGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:3783241/71‑1 (MQ=255) ggAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaaga < 1:3677035/71‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaagaa < 1:985296/71‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag > 1:1501300/1‑69 (MQ=255) aGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaagaag < 1:3589097/71‑1 (MQ=255) aGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaagaag < 1:3387611/71‑1 (MQ=255) gAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAggg < 1:453118/70‑1 (MQ=255) gAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAggg > 1:5292/1‑70 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCTCTGACATCATTGCGGGTTTGCGCAAGAAGGGaa > 1:2462483/1‑71 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCTCTGACATCATTGCGGGTTTGCGCAAGAAGGGaa > 1:3753808/1‑71 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatca > 1:303149/1‑47 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatca > 1:688801/1‑47 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatca > 1:315130/1‑47 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGa < 1:620448/70‑1 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCAGCTGAcatca > 1:790499/1‑47 (MQ=255) aGGAAAAATGGAAAGTAATTTCACTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGaa > 1:3032352/1‑71 (MQ=255) aaaaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaa > 1:2316716/1‑60 (MQ=255) aaaaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaa > 1:474747/1‑60 (MQ=255) aaaaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaa > 1:2184004/1‑60 (MQ=255) aaaaTTGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:1712915/71‑1 (MQ=255) aaaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:315634/71‑1 (MQ=255) aaaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:1544629/71‑1 (MQ=255) aaaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:2304915/71‑1 (MQ=255) aaaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:3068802/71‑1 (MQ=255) aaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:1081902/71‑1 (MQ=255) aaaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:3693990/71‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:715183/70‑1 (MQ=255) gTAATTTCATTGACTGGCATCCCGCTGACATCATTg > 1:955212/1‑36 (MQ=255) tAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:3133860/60‑1 (MQ=255) tAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:2160677/60‑1 (MQ=255) tAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:3732092/60‑1 (MQ=255) aaTTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaagaag > 1:1980720/1‑51 (MQ=255) aaTTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGcggcgg > 1:2900285/1‑70 (MQ=255) tCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAAtct > 1:3726175/1‑71 (MQ=255) tCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAAtc > 1:3687291/1‑70 (MQ=255) gACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAgg > 1:1754046/1‑43 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCTCAAGAAgg < 1:1836438/40‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAgg < 1:3316977/40‑1 (MQ=255) ggCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAAt > 1:2541301/1‑71 (MQ=255) ggCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAAt > 1:1325601/1‑71 (MQ=255) | GAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAAT > W3110S.gb/3334717‑3334856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |