| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,371,273 | Δ2 bp | 100% | intergenic (‑6/+299) | dcuA ← / ← aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,371,273 | 0 | A | . | 100.0% | 171.1 / NA | 44 | intergenic (‑6/+300) | dcuA/aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (25/19); total (25/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | W3110S.gb | 4,371,274 | 0 | A | . | 100.0% | 171.1 / NA | 44 | intergenic (‑7/+299) | dcuA/aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (25/19); total (25/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAA > W3110S.gb/4371203‑4371337 || gggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:826754/71‑1 (MQ=255)gggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:263136/71‑1 (MQ=255)gggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:2053482/71‑1 (MQ=255)gggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:1770575/71‑1 (MQ=255)gggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:1647264/71‑1 (MQ=255) gTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaa < 1:3133002/71‑1 (MQ=255) agcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt < 1:1089189/71‑1 (MQ=255) gcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa < 1:133989/71‑1 (MQ=255) cagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATaa > 1:3212793/1‑71 (MQ=255) gcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc > 1:638507/1‑71 (MQ=255) cTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:1642038/54‑1 (MQ=255) ttCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc < 1:2481321/69‑1 (MQ=255) tctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGAt < 1:3748582/70‑1 (MQ=255) atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg < 1:2684104/70‑1 (MQ=255) tAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa > 1:314224/1‑58 (MQ=255) aGTCCAGACCGCCAGCAACCTGCATGTCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt > 1:59993/1‑69 (MQ=255) aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGa > 1:3022911/1‑42 (MQ=38) aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAGCCGCCATGATatg > 1:3175804/1‑56 (MQ=255) aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATc > 1:2995399/1‑70 (MQ=255) aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATc > 1:1465585/1‑70 (MQ=255) gTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGa < 1:2198265/71‑1 (MQ=255) cAGACCTCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa > 1:2865810/1‑47 (MQ=37) cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa > 1:3504266/1‑47 (MQ=38) cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa > 1:1510232/1‑47 (MQ=38) cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg > 1:3315550/1‑71 (MQ=255) cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg > 1:1110416/1‑71 (MQ=255) cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg > 1:3099395/1‑71 (MQ=255) cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg > 1:2873104/1‑71 (MQ=255) cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg > 1:2009755/1‑71 (MQ=255) cAGACCGCCAGCAAACTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg > 1:2795932/1‑71 (MQ=255) aGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGAt < 1:968651/50‑1 (MQ=255) gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCTATAACCGCCATGATAATGGAGATGACATCGAACggg < 1:679506/71‑1 (MQ=255) gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACggg < 1:2249182/71‑1 (MQ=255) aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc < 1:3012105/42‑1 (MQ=37) aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc < 1:147687/42‑1 (MQ=37) aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc < 1:3789563/42‑1 (MQ=37) gCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATgg < 1:1121454/46‑1 (MQ=37) aaCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCgg < 1:952574/71‑1 (MQ=255) ccTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa > 1:2690500/1‑39 (MQ=37) atGCATGGCAG‑‑AAATAGCGGCGATAACCGCAATGATAATGGAGATGACATCGAACGGGa < 1:1692190/58‑1 (MQ=255) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGAt < 1:3016405/35‑1 (MQ=25) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGAt < 1:2403415/35‑1 (MQ=25) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa > 1:2808238/1‑53 (MQ=255) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt > 1:433731/1‑70 (MQ=255) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt > 1:2003597/1‑70 (MQ=255) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt > 1:3566944/1‑70 (MQ=255) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt > 1:746626/1‑70 (MQ=255) gCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTAc > 1:3036725/1‑63 (MQ=255) cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa > 1:2309654/1‑44 (MQ=38) cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGttt < 1:580617/69‑1 (MQ=255) cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTaa < 1:592686/71‑1 (MQ=255) || GGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAA > W3110S.gb/4371203‑4371337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |