New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	W3110S.gb	= 230556	47 (1.180)	14 (0.440) +CTCCTCAGCC	8/80	NT	34.3%	coding (326/915 nt)	yafC	predicted DNA‑binding transcriptional regulator
	?	W3110S.gb	1313528 =	20 (0.500)					intergenic (+6/+34)	tonB/yciA	membrane spanning protein in TonB‑ExbB‑ExbD complex/predicted hydrolase

GGCTTAATTAACGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/230541‑230556 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATT  >  W3110S.gb/1313528‑1313563                 ||||||||||                                     aaCTTAATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTC                        >  1:1490281/3‑40   CTTAATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTCC                       >  1:345461/1‑39   CTTAATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTC                        >  1:2863721/1‑38   CTTAATTAACGGCACTCCTCAGCCAAGTCAAAAGCCT                         <  1:3803193/37‑1   CTTAATTAACGGCACTCCTCAGCCAAGTCAAAAGCC                          <  1:2451739/36‑1    TTAATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTT         <  1:2514529/52‑1     TAATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGA       >  1:1351664/1‑53     TAATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTC                        >  1:158540/1‑36      AATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTAca  >  1:847068/1‑55       ATTAACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGGCGGAGGCTTT          <  1:3574222/48‑1        TTAACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGA                >  1:1586356/1‑41          AACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGA       >  1:586721/1‑48          AACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGA                >  1:1675932/1‑39          AACGGCACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGA                >  1:3681104/1‑39                ACTCCTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTA    <  1:3440577/45‑1                    CTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTAca  >  1:745026/1‑41                    CTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTA    <  1:3554717/41‑1                    CTCAGCCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTA    <  1:3661786/41‑1                 ||||||||||                                     GGCTTAATTAACGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/230541‑230556 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATT  >  W3110S.gb/1313528‑1313563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.