| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 31,629 | G→A | 7.7% | V271V (GTG→GTA) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 31,629 | 0 | G | A | 7.7% | 149.8 / 2.7 | 52 | V271V (GTG→GTA) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (20/28); new base A (2/2); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
CACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGG > W3110S.gb/31560‑31694 | cACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGtgc > 1:1950511/1‑71 (MQ=255) aCTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGtgct > 1:402944/1‑71 (MQ=255) aCTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGtgct > 1:3238376/1‑71 (MQ=255) aCTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGtgct > 1:1905451/1‑71 (MQ=255) aCTGTCGCGCCAGCCCAAACGCTGAACGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCTGtgct > 1:472458/1‑71 (MQ=255) aGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCg < 1:2095277/71‑1 (MQ=255) aGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCg < 1:1939528/71‑1 (MQ=255) cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGa > 1:3797953/1‑70 (MQ=255) tGACCGACAAAGAATACCAAATCATGCGTAACGCCTCGATGGCGGTACTGCGTGAAATCGGCGTTGAAAcc > 1:3350095/1‑71 (MQ=255) tGACCGACAAAGAATACCAAATCATGCGTAACGCCTCGATGGCGGTACTGCGTGAAATCGGCGTTGAAAc > 1:815649/1‑70 (MQ=255) aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAAt > 1:149175/1‑56 (MQ=255) aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAAcc < 1:2595103/69‑1 (MQ=255) aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAAcc < 1:3571040/69‑1 (MQ=255) aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCgg < 1:544484/71‑1 (MQ=255) aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCgg < 1:1164278/71‑1 (MQ=255) aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCgg < 1:910766/71‑1 (MQ=255) ccGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGc < 1:1326645/59‑1 (MQ=255) ccGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGc < 1:2417062/59‑1 (MQ=255) aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGaa < 1:1025437/50‑1 (MQ=255) aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGa < 1:792218/61‑1 (MQ=255) aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGa < 1:1138442/61‑1 (MQ=255) aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGa < 1:2660392/61‑1 (MQ=255) cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGtt > 1:3663819/1‑71 (MQ=255) aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGaaa < 1:3192192/48‑1 (MQ=255) aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTcc < 1:2808902/71‑1 (MQ=255) aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGAGCTGCGTGAAATCGGGGt > 1:929839/1‑55 (MQ=255) aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTcc > 1:2221457/1‑70 (MQ=255) gAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCaa > 1:2968158/1‑71 (MQ=255) tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGaa < 1:2456956/42‑1 (MQ=255) tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTcc > 1:3451024/1‑64 (MQ=255) tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTcc > 1:1983631/1‑64 (MQ=255) tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTcc > 1:1439093/1‑64 (MQ=255) tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTcc > 1:3138702/1‑64 (MQ=255) tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGtt > 1:2258090/1‑70 (MQ=255) tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGtt < 1:120012/70‑1 (MQ=255) aaaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAg > 1:266629/1‑71 (MQ=255) aaaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAg > 1:1842774/1‑71 (MQ=255) aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAAc < 1:3057209/50‑1 (MQ=255) cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCg > 1:3553024/1‑50 (MQ=255) cATGCGTAACGCCTCGATGGCGGTGCTGAGTGAAATCGGCGTTGAAACCg > 1:3194210/1‑50 (MQ=255) aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGc < 1:1364428/71‑1 (MQ=255) gTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTg < 1:876262/71‑1 (MQ=255) gTAACGCCTCGATGGCGGTACTGCGTGAAATCGGCGTTGAAACCggtgg < 1:113222/49‑1 (MQ=255) gTAACGCCTCGATGGCGGTACTGCGTGAAATCGGCGTTGAAACCggtgg < 1:1452545/49‑1 (MQ=255) aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTcc > 1:874184/1‑50 (MQ=255) aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAAc > 1:715683/1‑71 (MQ=255) cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGtt < 1:356221/57‑1 (MQ=255) cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGtt < 1:1198391/57‑1 (MQ=255) cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCg < 1:461432/70‑1 (MQ=255) cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTg > 1:1098582/1‑57 (MQ=255) cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGaa > 1:2350763/1‑70 (MQ=255) gATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGaaaa < 1:225666/71‑1 (MQ=255) gATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGaaa < 1:633013/70‑1 (MQ=255) gATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGaaa < 1:2406438/70‑1 (MQ=255) ggCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACg > 1:2913725/1‑71 (MQ=255) gCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACgg < 1:2289503/71‑1 (MQ=255) gCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACgg < 1:2702159/71‑1 (MQ=255) | CACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGG > W3110S.gb/31560‑31694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |