Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,951,613 A→G 7.7% R208R (CGT→CGC)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,951,6130AG 7.7% 138.6 / 2.8 52R208R (CGT→CGC) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (19/29);  new base G (2/2);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

AGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTAC  >  W3110S.gb/1951547‑1951683
                                                                  |                                                                      
aGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGt                                                                    >  1:2328460/1‑71 (MQ=255)
    aaTTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCaaa                                                                <  1:2051306/71‑1 (MQ=255)
       tCAGGCTGCCGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAAcc                                                             >  1:2959886/1‑71 (MQ=255)
       tCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAAAGATCTGATAGTAACGGTCAAAAcc                                                             >  1:3588744/1‑71 (MQ=255)
         aGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCgg                                                           <  1:2144134/71‑1 (MQ=255)
         aGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCgg                                                           <  1:1696917/71‑1 (MQ=255)
               aCGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAGCGGTCAAAGCCGGAcatca                                                     <  1:1500101/71‑1 (MQ=255)
               aCGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAGCGGTCAAAGCCGGAcatca                                                     <  1:3282497/71‑1 (MQ=255)
               aCGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCaaa                                                                <  1:769794/60‑1 (MQ=255)
                    cAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATcag                                                 >  1:1184182/1‑70 (MQ=255)
                        aCGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATcagca                                               >  1:327824/1‑68 (MQ=255)
                        aCGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATcagca                                               >  1:1868267/1‑68 (MQ=255)
                         cGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTg                                           >  1:1885410/1‑71 (MQ=255)
                              gTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATcagca                                               <  1:1289803/62‑1 (MQ=255)
                              gTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTg                                       <  1:3350590/70‑1 (MQ=255)
                              gTCCTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTg                                       <  1:17902/70‑1 (MQ=255)
                               tCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGa                                      <  1:2553641/70‑1 (MQ=255)
                               tCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGa                                      <  1:703512/70‑1 (MQ=255)
                                cTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCagcagc                                             >  1:1087324/1‑62 (MQ=255)
                                 ttCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCaaa                                                                <  1:3767062/42‑1 (MQ=255)
                                 ttCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCaaa                                                                <  1:3280349/42‑1 (MQ=255)
                                 ttCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCaaa                                                                <  1:2896064/42‑1 (MQ=255)
                                   cGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGCTTGaac                                    <  1:3655000/68‑1 (MQ=255)
                                     tCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTg                               <  1:1566291/71‑1 (MQ=255)
                                     tCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTg                               <  1:134974/71‑1 (MQ=255)
                                       aCGGAAGCATTTAACGATCTGATAGTAGCGGTCAAAGCCGGACATCATCAGTAGCTGTTTGAACAACTGCg                             >  1:3449378/1‑71 (MQ=255)
                                       aCGGAAGCATTTAACGATCTGATAGTAGCGGTCAAAGCCGGACATCATCAGTAGCTGTTTGAACAACTGCg                             >  1:1543137/1‑71 (MQ=255)
                                         ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCggg                           >  1:3045631/1‑71 (MQ=255)
                                         ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCggg                           <  1:3163686/71‑1 (MQ=255)
                                         ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGCAcatc                                                      >  1:1540613/1‑44 (MQ=255)
                                          gAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTg                                       <  1:380388/58‑1 (MQ=255)
                                              cATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTg                      <  1:2690133/71‑1 (MQ=255)
                                              cATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTg                      <  1:1713782/71‑1 (MQ=255)
                                                  tAACGATCTGATAGTAACGGTCAAAACCGGAcatcat                                                    >  1:1718474/1‑37 (MQ=255)
                                                  tAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGaac                                    >  1:869795/1‑53 (MQ=255)
                                                  tAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCgg                   >  1:859441/1‑70 (MQ=255)
                                                   aaCGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGaac                                    <  1:1809587/52‑1 (MQ=255)
                                                   aaCGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                  <  1:953202/70‑1 (MQ=255)
                                                   aaCGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                  <  1:632415/70‑1 (MQ=255)
                                                        tCTGATAGTAACGGTCAGAACCGGACATCATCAGCAGCTGTTTGaacaa                                  >  1:3224213/1‑49 (MQ=255)
                                                        tCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGaacaa                                  >  1:3376178/1‑49 (MQ=255)
                                                        tCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGaacaa                                  >  1:1103062/1‑49 (MQ=255)
                                                        tCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCg            >  1:3344347/1‑71 (MQ=255)
                                                          tGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTg                      <  1:3404542/59‑1 (MQ=255)
                                                          tGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTg                      <  1:3083209/59‑1 (MQ=255)
                                                            aTAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGaa                                     >  1:2451484/1‑42 (MQ=255)
                                                            aTAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGc             <  1:3464892/66‑1 (MQ=255)
                                                            aTAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAg         >  1:754011/1‑70 (MQ=255)
                                                            aTAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGa        <  1:1038855/71‑1 (MQ=255)
                                                            aTAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGa        <  1:327779/71‑1 (MQ=255)
                                                            aTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAg         <  1:530245/70‑1 (MQ=255)
                                                                tAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAAttt    <  1:985050/71‑1 (MQ=255)
                                                                 aaCGGTCAAAACCGGACATCATCAGCAGCTGTTTGaacaa                                  >  1:3276041/1‑40 (MQ=255)
                                                                  aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAg                >  1:2780313/1‑57 (MQ=255)
                                                                  aCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTAc  >  1:2044245/1‑71 (MQ=255)
                                                                  |                                                                      
AGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTAC  >  W3110S.gb/1951547‑1951683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑