| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,955,326 | T→G | 10.0% | L57L (CTT→CTG) | yecP → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,955,326 | 0 | T | G | 10.0% | 108.3 / 8.6 | 50 | L57L (CTT→CTG) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (15/30); new base G (3/2); total (18/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTG > W3110S.gb/1955260‑1955393 | gcaCGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGAtt > 1:2384670/1‑71 (MQ=255) gttTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCAt < 1:417621/70‑1 (MQ=255) tttAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGAtt < 1:927303/61‑1 (MQ=255) tttAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGAtt < 1:3051390/61‑1 (MQ=255) tttAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGAtt < 1:2009823/61‑1 (MQ=255) ttAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGc > 1:1296655/1‑71 (MQ=255) ttAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGc > 1:1465517/1‑71 (MQ=255) ttAAGCAGTGGTCAAATGCGGTGGAGTTTCTACATGAAATTAAACCGTATCGTCTTGATTTATTGCATAGc > 1:3471573/1‑71 (MQ=255) tAAGCAGTGGTCCAACGCGGTGGAATTTCTGCCTGAAATTAAACCGTATCGTCTGGATTTATTGCATATCg > 1:1502702/1‑71 (MQ=255) tAAGCAGTGGTCCAACGCGGTGGAATTTCTGCCTGAAATTAAACCGTATCGTCTGGATTTATTGCATAGCg > 1:3181622/1‑71 (MQ=255) tAAGCAGTGGTCCAACGCGGTGGAATTTCTGCCTGAAATTAAACCGTATCGTCTGGATTTATTGCATAGCg > 1:2070267/1‑71 (MQ=255) aGCAGTGGTCTAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGt < 1:1618042/70‑1 (MQ=255) cAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTAc < 1:133387/71‑1 (MQ=255) tGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGt < 1:862471/65‑1 (MQ=255) gTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACgg > 1:1236413/1‑68 (MQ=255) gTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACgg > 1:2373730/1‑68 (MQ=255) aaaTGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGa < 1:553151/69‑1 (MQ=255) aaaTGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGa < 1:3048189/69‑1 (MQ=255) aaTGCGGTGGAGTTTCTAGCTGAAATTAAACCGTATCGTCTTGAttt < 1:1267539/47‑1 (MQ=255) aaTGCGGTGGAGTTTCTAGCTGAAATTAAACCGTATCGTCTTGAttt < 1:1272129/47‑1 (MQ=255) aaTGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGAttt < 1:834271/47‑1 (MQ=255) aaTGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGAttt < 1:1429297/47‑1 (MQ=255) gAGTTTCTACCTGAAATTAAGCCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCg < 1:3543717/64‑1 (MQ=255) gAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGc < 1:665967/70‑1 (MQ=255) gTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGtt < 1:2013178/49‑1 (MQ=255) tttCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGa < 1:1973487/62‑1 (MQ=255) ttCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGa < 1:2037115/61‑1 (MQ=255) ttCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACt > 1:992541/1‑70 (MQ=255) tCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACt > 1:2923420/1‑69 (MQ=255) tCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACt > 1:1541826/1‑69 (MQ=255) ccTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGt < 1:3107826/41‑1 (MQ=255) ccTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAgcg < 1:1939195/70‑1 (MQ=255) cTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGcc < 1:2777421/61‑1 (MQ=255) cTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCa < 1:199194/71‑1 (MQ=255) cTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCa < 1:595322/71‑1 (MQ=255) cTGAAATTAAACCGTATCGTCTTGATTTATTGCATACCGTTACGGCAGAAAGCGAAGAGcc < 1:1522865/61‑1 (MQ=255) gAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAgg > 1:2267896/1‑71 (MQ=255) gAAATTAAACCGTATCGTCTGGATTTATTGCATAGCGta < 1:1519097/39‑2 (MQ=38) gAAATTAAACCGTATCGTCTGGATTTATTGCATAGCGta < 1:20816/39‑2 (MQ=38) aaaTTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAgg < 1:3315492/70‑1 (MQ=255) aTTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCg < 1:2594480/49‑1 (MQ=255) aTTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGc > 1:1083880/1‑70 (MQ=255) tAAACCGTATCGTGTTGATTTATTGCATAGCGTTACGGCAGAAAGCGa > 1:3481127/1‑48 (MQ=255) aaaCCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAAt > 1:1574082/1‑71 (MQ=255) aCCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTa < 1:2501283/71‑1 (MQ=255) tCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCg < 1:929411/38‑1 (MQ=255) tCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCTGGGCAAATTAAACGc > 1:442986/1‑70 (MQ=255) tCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTg < 1:2797432/71‑1 (MQ=255) cTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTg > 1:3338213/1‑70 (MQ=255) cTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTg < 1:2273096/70‑1 (MQ=255) | GCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTG > W3110S.gb/1955260‑1955393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |