| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,969,921 | G→A | 22.7% | L99L (CTG→TTG) | cheB ← | fused chemotaxis regulator and protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,969,921 | 0 | G | A | 22.7% | 36.2 / 7.0 | 22 | L99L (CTG→TTG) | cheB | fused chemotaxis regulator and protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Reads supporting (aligned to +/- strand): ref base G (7/10); new base A (3/2); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
ATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTG > W3110S.gb/1969851‑1969989 | aTTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAg > 1:3646087/1‑71 (MQ=255) taCGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAgcg < 1:423672/70‑1 (MQ=255) gCATTCCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAACTCCAgcgc < 1:3803452/64‑1 (MQ=255) gCATTCCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAACTCCAgcgc < 1:2704345/64‑1 (MQ=255) cATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGc < 1:1650734/70‑1 (MQ=255) aTACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGAccc < 1:382020/71‑1 (MQ=255) cAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGt < 1:1903780/54‑1 (MQ=255) gTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTc < 1:2539898/37‑1 (MQ=255) tGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGt > 1:2939815/1‑71 (MQ=255) tGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGt > 1:1716371/1‑71 (MQ=255) cGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGt > 1:1204002/1‑69 (MQ=255) ttGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAggg < 1:834159/70‑1 (MQ=255) tGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAggg > 1:2865034/1‑69 (MQ=255) tGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAggg > 1:3452688/1‑69 (MQ=255) tGGTGACAAAATCTATCGCCCCCAACTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAggg > 1:1834248/1‑69 (MQ=255) aCAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGgaa > 1:396893/1‑66 (MQ=255) aaTCTATCGCCCCCAACTCCAGCGCGCGCAGCGTGACTTCTg > 1:1967688/1‑42 (MQ=255) aaTCTATCGCCCCCAACTCCAGCGCGCGCAGCGTGACTTCTg > 1:556579/1‑42 (MQ=255) tCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAAc < 1:2737315/70‑1 (MQ=255) ccAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCAtt < 1:1446992/71‑1 (MQ=255) ccAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCAtt < 1:1298350/71‑1 (MQ=255) cAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTg > 1:2755072/1‑71 (MQ=255) cAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTg < 1:1451756/71‑1 (MQ=255) | ATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTG > W3110S.gb/1969851‑1969989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |