| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,973,098 | G→A | 12.0% | A436A (GCC→GCT) | tar ← | methyl‑accepting chemotaxis protein II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,973,098 | 0 | G | A | 12.0% | 130.7 / 7.7 | 50 | A436A (GCC→GCT) | tar | methyl‑accepting chemotaxis protein II |
| Reads supporting (aligned to +/- strand): ref base G (19/25); new base A (4/2); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
ATCTCGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTCAATG > W3110S.gb/1973036‑1973152 | aTCTCGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTc < 1:479855/71‑1 (MQ=255)aTCTCGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTc < 1:1006218/71‑1 (MQ=255) tctcGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTc > 1:2332390/1‑70 (MQ=255) tctcGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTc > 1:1992675/1‑70 (MQ=255) tctcGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTc > 1:3192184/1‑70 (MQ=255) tctcGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTc > 1:2692842/1‑70 (MQ=255) gCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCg < 1:2712765/67‑1 (MQ=255) gCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGAc < 1:1131026/69‑1 (MQ=255) tAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGgcgc < 1:65025/57‑1 (MQ=255) aTGTCAGTCACGCTAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCAGCGCTTTAGACCAGCACCGa < 1:2630569/71‑1 (MQ=38) aTGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCa > 1:670023/1‑67 (MQ=255) aTGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCa > 1:1715214/1‑67 (MQ=255) aTGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCa > 1:3456527/1‑67 (MQ=255) aTGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCg < 1:1875607/70‑1 (MQ=255) aTGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCAGCGCTTTCGACCAGCACCGa < 1:2927454/71‑1 (MQ=255) agtcaCGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCg < 1:1080397/70‑1 (MQ=255) aCGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGAACAGCACCGAACCGGTAt > 1:3686000/1‑70 (MQ=255) cgAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCaa > 1:1978770/1‑70 (MQ=255) aCAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGt > 1:105753/1‑69 (MQ=255) cATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACg < 1:2056653/71‑1 (MQ=255) cATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACg < 1:1409974/71‑1 (MQ=255) aTTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGa > 1:3548859/1‑38 (MQ=255) ttGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGt < 1:3574753/63‑1 (MQ=255) ttGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACg > 1:1818890/1‑69 (MQ=255) ttGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGa < 1:2378877/71‑1 (MQ=255) ttGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGa < 1:3820402/71‑1 (MQ=255) ttGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGa < 1:3012786/71‑1 (MQ=255) gACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTga < 1:2855934/63‑1 (MQ=255) gACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGt > 1:1672640/1‑71 (MQ=255) gACGATATTGTTCATTGTTTCCCCAGCGCTTTCGa > 1:370620/1‑35 (MQ=255) gACGATATTGTTCATTGTTTCCCCAGCGCTTTCGa > 1:2056584/1‑35 (MQ=255) gACGATATTGTTCATTGTTTCCCCAGCGCTTTCGa > 1:290090/1‑35 (MQ=255) gACGATATTGTTCATTGTTTCCCCAGCGCTTTCGa > 1:3361731/1‑35 (MQ=255) gATATTGTTCATTGTTT‑CCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACg < 1:3184473/63‑1 (MQ=255) atatTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTc < 1:1539787/71‑1 (MQ=255) ttGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATc < 1:2625920/47‑1 (MQ=255) ttGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTCAAt < 1:1751005/71‑1 (MQ=255) ttGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTCAAt < 1:2516448/71‑1 (MQ=255) tGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTCAAt < 1:2181186/70‑1 (MQ=255) gTTCATTGTTTCCCCGGCGCTTTCGACCAGCCCCg > 1:2960769/1‑35 (MQ=255) gTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAAc > 1:1302716/1‑38 (MQ=255) gTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTCAATg < 1:956308/70‑1 (MQ=255) gTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTCAATg < 1:268854/70‑1 (MQ=255) ttCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTc < 1:3579484/62‑1 (MQ=255) ttCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTc < 1:2188657/62‑1 (MQ=255) ttCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTc < 1:1338052/62‑1 (MQ=255) tCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTa > 1:1569414/1‑41 (MQ=255) tGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTgag > 1:3607683/1‑49 (MQ=255) tGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTgag > 1:374465/1‑49 (MQ=255) tGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTgag > 1:3542378/1‑49 (MQ=255) | ATCTCGCCCATAATGTCAGTCACGCGAGTGACAGCATTGACGATATTGTTCATTGTTTCCCCGGCGCTTTCGACCAGCACCGAACCGGTATCAACGCGTGAGACGGAGTCTTCAATG > W3110S.gb/1973036‑1973152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |