| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,979,442 | G→A | 18.1% | R39R (CGC→CGT) | flhC ← | DNA‑binding transcriptional regulator with FlhD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,979,442 | 0 | G | A | 18.1% | 44.8 / 3.2 | 22 | R39R (CGC→CGT) | flhC | DNA‑binding transcriptional regulator with FlhD |
| Reads supporting (aligned to +/- strand): ref base G (5/13); new base A (2/2); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGATCAATTCCATT > W3110S.gb/1979376‑1979511 | ggTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGt < 1:1913611/70‑1 (MQ=255) gagaATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCcgc < 1:2033951/70‑1 (MQ=255) aTGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACtt < 1:858834/71‑1 (MQ=255) gcagcaTGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAAc < 1:1579225/71‑1 (MQ=255) tCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCt < 1:1487326/70‑1 (MQ=255) ggCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCAt < 1:918844/70‑1 (MQ=255) ggCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATc < 1:1649988/71‑1 (MQ=255) tCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCAt > 1:280508/1‑66 (MQ=255) tCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACt > 1:2163041/1‑37 (MQ=255) tCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCa > 1:1006207/1‑55 (MQ=255) cTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCa > 1:2636204/1‑60 (MQ=255) cTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGcc > 1:2664377/1‑70 (MQ=255) tttATAGAGTTTTATCAGACGTCCGCGACTTAACTGTGTTTCGCTTTCCAGc < 1:3666519/52‑1 (MQ=255) tataAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAATCGAGCGCCCa < 1:2466480/69‑1 (MQ=255) tataAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCa < 1:1237714/69‑1 (MQ=255) gTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGATc < 1:2668242/71‑1 (MQ=255) gTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGATc < 1:3646785/71‑1 (MQ=255) gTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGATc < 1:181491/71‑1 (MQ=255) ttttATCAGACGTCCGCGACTTAACTGTGTTTCGCTTTCCAGc < 1:3449603/43‑1 (MQ=255) aTCAGACGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAgg > 1:3234872/1‑60 (MQ=255) aTCAGACGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAgg > 1:1992478/1‑60 (MQ=255) aGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGATCAATTCCa < 1:2210005/70‑1 (MQ=255) ggCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTCCAAACGAGCGCCCAGGGTGATCAATTCCAtt < 1:3442426/71‑1 (MQ=255) | GGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGATCAATTCCATT > W3110S.gb/1979376‑1979511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |