| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,089,667 | A→C | 11.2% | R243R (CGT→CGG) | yeeY ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,089,667 | 0 | A | C | 11.2% | 70.5 / 6.0 | 36 | R243R (CGT→CGG) | yeeY | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (18/13); new base C (2/2); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
AAGAGAGTTTGGGCGACCACTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGAAAACGGT > W3110S.gb/2089599‑2089735 | aaGAGAGTTTGGGCGACCACTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACg < 1:418307/71‑1 (MQ=255) agagTTTGGGCGACCACTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACggg > 1:1969613/1‑70 (MQ=255) gACCACTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATACCGTGGTAAATAg > 1:154607/1‑67 (MQ=255) gACCACTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATACCGTGGTAAATAg > 1:2526716/1‑67 (MQ=255) cTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGcc > 1:3686977/1‑70 (MQ=255) cTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGcc > 1:3256130/1‑70 (MQ=255) tttCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACg < 1:399266/51‑1 (MQ=255) ttcttcTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAg > 1:2624169/1‑71 (MQ=255) tcttctCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATATCGGGGTaa < 1:1471626/55‑1 (MQ=255) tcttctCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAgg > 1:2726946/1‑71 (MQ=255) tcttctCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAgg > 1:1286918/1‑71 (MQ=255) ctcGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAAt > 1:1068286/1‑53 (MQ=255) gATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAAc > 1:1972807/1‑41 (MQ=255) gATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAAc > 1:1968490/1‑41 (MQ=255) cTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAg > 1:3248311/1‑71 (MQ=255) tATCGAGAAAACGTTGCGCCAGATACCGTGGTAAATAGCCGCAGCCAAGGCCGCTAATTTGc < 1:3430695/62‑1 (MQ=255) tATCGAGAAAACGTTGCGCCAGATACCGTGGTAAATAGCCGCAGCCAAGGCCGCTAATTTGc < 1:2762008/62‑1 (MQ=255) tCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCa < 1:2555864/61‑1 (MQ=255) gagaAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGc > 1:780322/1‑61 (MQ=255) gagaAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGc > 1:2937682/1‑61 (MQ=255) aaaCGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGttt > 1:1636012/1‑70 (MQ=255) aaCGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCa > 1:2662180/1‑54 (MQ=255) aaCGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGtttt > 1:3617999/1‑70 (MQ=255) cGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTaaa < 1:890281/71‑1 (MQ=255) cGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTaa > 1:1317192/1‑70 (MQ=255) gTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCa > 1:3170185/1‑51 (MQ=255) gTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTaaaa < 1:1889344/71‑1 (MQ=255) gTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTaaaa < 1:636945/71‑1 (MQ=255) gcCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGaa < 1:201513/71‑1 (MQ=255) gcCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGaa < 1:720464/71‑1 (MQ=255) ccAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTaaa < 1:117599/64‑1 (MQ=255) tAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAg > 1:1144257/1‑41 (MQ=255) tAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGAAAACGGt < 1:2958176/71‑1 (MQ=255) tAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGAAAACGGt < 1:3190305/71‑1 (MQ=255) tAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGAAAACGGt < 1:1057355/71‑1 (MQ=255) tAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGAAAACGGt < 1:452377/71‑1 (MQ=255) aaCGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGAAAACGGt < 1:2268684/70‑1 (MQ=255) | AAGAGAGTTTGGGCGACCACTTTCTTCTCGATTAACGCGCCACTATCGAGAAAACGTTGCGCCAGATAACGGGGTAAATAGCCGCAGCCGAGGCCGCTAATTTGCAGCTCCAGTTTGGTTTTAAAATCGAAAACGGT > W3110S.gb/2089599‑2089735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |