| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,119,294 | C→T | 23.2% | Q416Q (CAG→CAA) | wcaK ← | predicted pyruvyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,119,294 | 0 | C | T | 23.2% | 77.6 / 19.6 | 43 | Q416Q (CAG→CAA) | wcaK | predicted pyruvyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (18/15); new base T (6/4); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGC > W3110S.gb/2119235‑2119359 | gAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTg < 1:1602571/71‑1 (MQ=255)gAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTg < 1:2695483/71‑1 (MQ=255) aaTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTg > 1:1716309/1‑70 (MQ=255) aaaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCt < 1:3788577/71‑1 (MQ=255) agaagCCGACCTTCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTccc > 1:1952566/1‑63 (MQ=255) gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTc < 1:731362/71‑1 (MQ=255) cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:925139/71‑1 (MQ=255) cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:1941588/71‑1 (MQ=255) cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:3431443/71‑1 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:1633846/66‑3 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:413721/66‑3 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:3550499/66‑3 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:2618492/66‑3 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:2876005/65‑1 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGa > 1:1283918/1‑67 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGa > 1:487594/1‑67 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGa > 1:2121304/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGAc > 1:10062/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGAc > 1:92340/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGAc > 1:1978661/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGATCGCGAc > 1:1403891/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGa > 1:3230896/1‑70 (MQ=255) cATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGa > 1:613996/1‑70 (MQ=255) cATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc > 1:2671621/1‑71 (MQ=255) aCCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt < 1:2592283/70‑1 (MQ=255) aCCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt < 1:844057/70‑1 (MQ=255) cTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc > 1:3129392/1‑71 (MQ=255) cTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc > 1:1795821/1‑71 (MQ=255) ccccGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt > 1:2829198/1‑70 (MQ=255) ccccGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt > 1:2849266/1‑70 (MQ=255) cccGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGATTCACt > 1:3253535/1‑69 (MQ=255) cccGATGCGCTCAAGCACAGACTGAACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt > 1:845578/1‑69 (MQ=255) cGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGa > 1:2474880/1‑44 (MQ=255) cGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGAc > 1:2637940/1‑54 (MQ=255) tGCGCTCAAGCACAGACTGCACCATCTGCATTCCt > 1:705444/1‑35 (MQ=255) gcgcTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg > 1:2339735/1‑71 (MQ=255) cgcTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACg > 1:1504743/1‑54 (MQ=255) cgcTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg < 1:3489712/70‑1 (MQ=255) aaGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:3535425/70‑1 (MQ=255) aaGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:1726601/70‑1 (MQ=255) aaGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:2769581/70‑1 (MQ=255) acaGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAgcgc > 1:3317742/1‑71 (MQ=255) acaGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc < 1:328746/71‑1 (MQ=255) | GAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGC > W3110S.gb/2119235‑2119359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |