| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,119,321 | C→T | 12.8% | E407E (GAG→GAA) | wcaK ← | predicted pyruvyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,119,321 | 0 | C | T | 12.8% | 157.8 / 12.9 | 63 | E407E (GAG→GAA) | wcaK | predicted pyruvyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (27/27); new base T (4/4); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATC > W3110S.gb/2119253‑2119390 | gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTc < 1:731362/71‑1 (MQ=255) cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:925139/71‑1 (MQ=255) cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:3431443/71‑1 (MQ=255) cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:1941588/71‑1 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:3550499/66‑3 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:2618492/66‑3 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:413721/66‑3 (MQ=255) ttCATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTcgc < 1:1633846/66‑3 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCAc < 1:2876005/65‑1 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGa > 1:487594/1‑67 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGa > 1:2121304/1‑67 (MQ=255) tCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGa > 1:1283918/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGAc > 1:10062/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGAc > 1:1978661/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGAc > 1:92340/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGTTCAAGCACAGATTGCACCATCTGCATTCCCGTCTGACGATCGCGAc > 1:1403891/1‑67 (MQ=255) cATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGa > 1:3230896/1‑70 (MQ=255) cATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGa > 1:613996/1‑70 (MQ=255) cATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc > 1:2671621/1‑71 (MQ=255) aCCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt < 1:844057/70‑1 (MQ=255) aCCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt < 1:2592283/70‑1 (MQ=255) cTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc > 1:1795821/1‑71 (MQ=255) cTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc > 1:3129392/1‑71 (MQ=255) ccccGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt > 1:2849266/1‑70 (MQ=255) ccccGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt > 1:2829198/1‑70 (MQ=255) cccGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGATTCACt > 1:3253535/1‑69 (MQ=255) cccGATGCGCTCAAGCACAGACTGAACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt > 1:845578/1‑69 (MQ=255) cGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGAc > 1:2637940/1‑54 (MQ=255) gcgcTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg > 1:2339735/1‑71 (MQ=255) cgcTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACg > 1:1504743/1‑54 (MQ=255) cgcTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg < 1:3489712/70‑1 (MQ=255) aaGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:3535425/70‑1 (MQ=255) aaGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:2769581/70‑1 (MQ=255) aaGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:1726601/70‑1 (MQ=255) acaGATTGCACCATCTGCATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAgcgc > 1:3317742/1‑71 (MQ=255) acaGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc < 1:328746/71‑1 (MQ=255) cACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCa > 1:2568473/1‑43 (MQ=255) cACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCt > 1:3432483/1‑71 (MQ=255) cACCATATGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcg > 1:492601/1‑51 (MQ=255) ccATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:2539554/1‑53 (MQ=255) ccATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:402197/1‑53 (MQ=255) cTGCATTCCTGTCTGACGCTCACGACTGACGGCTTc > 1:16700/1‑36 (MQ=255) cTGCATTCCTGTCTGACGCTCACGACTGACGGCTTc > 1:2217601/1‑36 (MQ=255) cTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg > 1:2241975/1‑54 (MQ=255) tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:1015431/51‑1 (MQ=255) tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc < 1:2792994/51‑1 (MQ=255) tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCt > 1:1087228/1‑69 (MQ=255) cATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTa < 1:1914233/68‑1 (MQ=255) cATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTa < 1:3241606/68‑1 (MQ=255) cATTCCTGTCTGACGCTCACGACTGACGGCCTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAgg < 1:2458027/71‑1 (MQ=255) aTTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTa < 1:2126944/67‑1 (MQ=255) aTTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt < 1:1626519/71‑1 (MQ=255) aTTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt < 1:3285587/71‑1 (MQ=255) aTTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt < 1:828416/71‑1 (MQ=255) ttCCTGTCTGACGCTCACGACTGACGGCTTCACTAAg < 1:688330/37‑1 (MQ=255) ttCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa < 1:3541453/71‑1 (MQ=255) ccTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATc > 1:235272/1‑71 (MQ=255) ccTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATc > 1:2120416/1‑71 (MQ=255) cTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATcc > 1:1651465/1‑71 (MQ=255) tCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:2220504/70‑1 (MQ=255) aCGCTCACGACTGACGGCTTCACTAAGTCGCTCATTCAGCGCCGGAAGCTGGCCTAAGGTATCTGCAACCa < 1:820570/71‑1 (MQ=255) gCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATc < 1:1523281/71‑1 (MQ=255) gCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATc < 1:918151/71‑1 (MQ=255) | GCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATC > W3110S.gb/2119253‑2119390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |