| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,122,101 | C→G | 11.5% | S65S (TCG→TCC) | wzxC ← | colanic acid exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,122,101 | 0 | C | G | 11.5% | 152.7 / 9.6 | 61 | S65S (TCG→TCC) | wzxC | colanic acid exporter |
| Reads supporting (aligned to +/- strand): ref base C (29/24); new base G (4/3); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01 | |||||||||||
TCCCCAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAG > W3110S.gb/2122031‑2122169 | tcgccAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATc < 1:142436/68‑1 (MQ=255)tCCCCAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATc < 1:65065/71‑1 (MQ=255) cccAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATc > 1:2280276/1‑69 (MQ=255) cccAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCg < 1:1123963/70‑1 (MQ=255) cccAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGa < 1:26886/71‑1 (MQ=255) cccAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGa < 1:3493484/71‑1 (MQ=255) cAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGt > 1:1847693/1‑71 (MQ=255) cAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGt > 1:2539589/1‑71 (MQ=255) cAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGt < 1:1190425/71‑1 (MQ=255) ccGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGt < 1:845200/67‑1 (MQ=255) ccGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGc < 1:2027896/71‑1 (MQ=255) aCGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTATCGCTGAATAATCGAGATAGCGa > 1:2331842/1‑70 (MQ=255) cAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCg > 1:3225178/1‑70 (MQ=255) ccAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGt < 1:148691/71‑1 (MQ=255) gTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGt < 1:3328990/68‑1 (MQ=255) gTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAg < 1:1293850/71‑1 (MQ=255) cAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGa > 1:1673860/1‑56 (MQ=255) cAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGa > 1:638354/1‑69 (MQ=255) cAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGa > 1:3259939/1‑69 (MQ=255) cAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGa > 1:3634674/1‑69 (MQ=255) aCGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGt > 1:418606/1‑63 (MQ=255) aCGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGt > 1:1837616/1‑63 (MQ=255) gtggtgAGTTCAAGGTGAGTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGc > 1:3034094/1‑69 (MQ=255) gtggtgAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGc > 1:2806837/1‑50 (MQ=255) gtggtgAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGt < 1:184242/71‑1 (MQ=255) tgAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCt < 1:2521729/71‑1 (MQ=255) aGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGc > 1:710953/1‑71 (MQ=255) ttCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAg < 1:2789549/37‑1 (MQ=255) ttCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAg < 1:2112839/37‑1 (MQ=255) ttCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAg < 1:872092/37‑1 (MQ=255) ttCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAg < 1:3801762/37‑1 (MQ=255) ttCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGc > 1:2793990/1‑69 (MQ=255) cAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGa > 1:2954240/1‑42 (MQ=255) cAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAgc > 1:1443742/1‑71 (MQ=255) cAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGAGATACCGAAGTCAGAAAGCGTATCTGCCAgc > 1:1943205/1‑71 (MQ=255) aGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGc < 1:164067/38‑1 (MQ=255) ggTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGcgcg < 1:3532694/71‑1 (MQ=255) tGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGa > 1:673010/1‑70 (MQ=255) gACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGaa > 1:2176708/1‑43 (MQ=255) aCTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATaa > 1:2924625/1‑71 (MQ=255) gATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCa > 1:3233737/1‑71 (MQ=255) tttCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTa < 1:2043577/50‑1 (MQ=255) tCTTTTCGCTGAATAATCGAGTTAGCGATACCGaa > 1:2492029/1‑35 (MQ=255) tCTTTTCGCTGAATAATCGAGTTAGCGATACCGaa > 1:1957891/1‑35 (MQ=255) ttCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTAt > 1:2832664/1‑45 (MQ=255) ttCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTAt > 1:1264458/1‑45 (MQ=255) ttCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATATTCACCAGCGa > 1:27246/1‑70 (MQ=255) ttCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGa > 1:2581279/1‑70 (MQ=255) tCGCTGAATAATAGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGaca > 1:3199720/1‑71 (MQ=255) cTGAATAATGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATCACCAGCGacacag < 1:197859/71‑3 (MQ=255) cTGAATAATGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATCACCAGCGacacag < 1:977366/71‑3 (MQ=255) tGAATAATGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATCACCAGCGacacag < 1:2686239/70‑3 (MQ=255) aataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGt < 1:2141850/69‑1 (MQ=255) aataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGt < 1:1847007/69‑1 (MQ=255) ataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAg < 1:2640425/71‑1 (MQ=255) ataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAg < 1:2950139/71‑1 (MQ=255) ataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAg > 1:1382862/1‑71 (MQ=255) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:3657794/1‑52 (MQ=38) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:2500120/1‑52 (MQ=38) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:152949/1‑52 (MQ=38) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:1486312/1‑52 (MQ=38) atCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAg > 1:745882/1‑71 (MQ=255) atCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAg < 1:1152296/71‑1 (MQ=255) tCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCAc < 1:951094/52‑1 (MQ=255) | TCCCCAGCCCGACGTTCAGCCAGTACAACGTGGTGAGTTCAAGGTGACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAG > W3110S.gb/2122031‑2122169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |