| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,122,146 | A→G | 13.2% | I50I (ATT→ATC) | wzxC ← | colanic acid exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,122,146 | 0 | A | G | 13.2% | 128.6 / 11.1 | 53 | I50I (ATT→ATC) | wzxC | colanic acid exporter |
| Reads supporting (aligned to +/- strand): ref base A (24/22); new base G (4/3); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
ACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCACGGTCATCTGC > W3110S.gb/2122077‑2122215 | aCTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATaa > 1:2924625/1‑71 (MQ=255) gATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCa > 1:3233737/1‑71 (MQ=255) ttCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATATTCACCAGCGa > 1:27246/1‑70 (MQ=255) ttCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGa > 1:2581279/1‑70 (MQ=255) tCGCTGAATAATAGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGaca > 1:3199720/1‑71 (MQ=255) cTGAATAATGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATCACCAGCGacacag < 1:977366/71‑3 (MQ=255) cTGAATAATGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATCACCAGCGacacag < 1:197859/71‑3 (MQ=255) tGAATAATGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATCACCAGCGacacag < 1:2686239/70‑3 (MQ=255) aataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGt < 1:2141850/69‑1 (MQ=255) aataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGt < 1:1847007/69‑1 (MQ=255) ataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAg < 1:2950139/71‑1 (MQ=255) ataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAg < 1:2640425/71‑1 (MQ=255) ataatCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAg > 1:1382862/1‑71 (MQ=255) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:152949/1‑52 (MQ=38) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:1486312/1‑52 (MQ=38) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:3657794/1‑52 (MQ=38) aatGGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCAATGATc > 1:2500120/1‑52 (MQ=38) atCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAg < 1:1152296/71‑1 (MQ=255) atCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAg > 1:745882/1‑71 (MQ=255) tCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCAc < 1:951094/52‑1 (MQ=255) gAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGcc > 1:2243459/1‑71 (MQ=255) gAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGcc > 1:998497/1‑71 (MQ=255) aGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCg < 1:311692/71‑1 (MQ=255) aGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCg < 1:2741337/71‑1 (MQ=255) aGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACt > 1:746782/1‑71 (MQ=255) aGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACt > 1:3203799/1‑71 (MQ=255) gCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCa < 1:2106873/43‑1 (MQ=255) gCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACtg > 1:2506016/1‑71 (MQ=255) gTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTTGTTGTCGa > 1:2934084/1‑71 (MQ=255) gTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGa < 1:3091656/71‑1 (MQ=255) aGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATaa > 1:2175301/1‑71 (MQ=255) gAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCATGCCg < 1:228143/51‑1 (MQ=255) aaaGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATc > 1:150599/1‑71 (MQ=255) aaaGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATc > 1:2128846/1‑71 (MQ=255) aaGCGTATCTGCCAGCGCGATAATCACCA‑‑GACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCcgc > 1:1071938/1‑71 (MQ=255) aGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCcg > 1:3197547/1‑71 (MQ=255) cGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCcgcg > 1:954711/1‑71 (MQ=255) cGTAACTGGCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACtgg > 1:3306015/1‑52 (MQ=39) tCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGa < 1:3522085/43‑1 (MQ=255) tCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGa < 1:46704/43‑1 (MQ=255) tCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAg > 1:191276/1‑71 (MQ=255) tGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCa > 1:1842792/1‑71 (MQ=255) gcaaGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCa > 1:1479658/4‑70 (MQ=255) ccAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAg < 1:3618746/67‑1 (MQ=255) gcgcGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGa < 1:2641477/51‑1 (MQ=255) gcgcGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGa < 1:1378472/51‑1 (MQ=255) gcgcGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGa < 1:92666/51‑1 (MQ=255) gcgcGATAATCAACAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGa < 1:1820334/51‑1 (MQ=255) gcgATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCACGGTCAt > 1:943476/1‑71 (MQ=255) cgATAATCACCAGCGACACGGTAAGCAGGCCGAACtggtg > 1:2818573/1‑40 (MQ=255) gATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCACGGTCATc < 1:2118646/70‑1 (MQ=255) aTAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTc < 1:1376958/44‑1 (MQ=255) tAATCACCAGCGACACGGTAAGCATGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCACGGTCATCTGc < 1:2428753/71‑1 (MQ=255) tAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCACGGTCATCTGc < 1:3261619/71‑1 (MQ=255) | ACTGATTTCTTTTCGCTGAATAATCGAGTTAGCGATACCGAAGTCAGAAAGCGTATCTGCCAGCGCGATAATCACCAGCGACACGGTAAGCAGGCCGAACTGGTGGTTGTCGATAATCCGCGCCAGCACGGTCATCTGC > W3110S.gb/2122077‑2122215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |