| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,127,320 | G→A | 6.1% | A188A (GCC→GCT) | wcaI ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,127,320 | 0 | G | A | 6.1% | 193.2 / 2.3 | 66 | A188A (GCC→GCT) | wcaI | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (30/30); new base A (2/2); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
AAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCG > W3110S.gb/2127254‑2127386 | aaaaCGGGCAATTTCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCtt < 1:3696735/71‑1 (MQ=255) tttCTGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATAGCTTTATTcatcatt < 1:1536878/71‑2 (MQ=255) tttCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTAtt < 1:3134967/64‑1 (MQ=255) tttCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTAtt < 1:1203628/64‑1 (MQ=255) tttCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTAtt < 1:762747/64‑1 (MQ=255) tttCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCatcatc > 1:1893115/1‑71 (MQ=255) tCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCatcatc > 1:2738547/1‑69 (MQ=255) tCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCatcatc > 1:1249812/1‑69 (MQ=255) tCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCg > 1:3370913/1‑70 (MQ=255) tCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCg > 1:701238/1‑70 (MQ=255) tCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCg > 1:2023058/1‑70 (MQ=255) tCCGACCAGTTGGGG‑AGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGaa > 1:836940/1‑71 (MQ=255) ctcGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCg < 1:1013119/68‑1 (MQ=255) gACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCg < 1:130844/67‑1 (MQ=255) gACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCAACGCCTTTTTCGATGGCTTTATTCATCATCg < 1:1888212/67‑1 (MQ=255) tGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAAcgc > 1:821308/1‑65 (MQ=255) tGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAAcgc > 1:1998439/1‑65 (MQ=255) tGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATc > 1:335888/1‑71 (MQ=255) ggggAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCg > 1:1222052/1‑71 (MQ=255) gggAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCg < 1:226880/70‑1 (MQ=255) gggAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCg < 1:1256289/70‑1 (MQ=255) ggAAGAAGATGACGTTTTCCGCCGCCACGCCTTTCTCGATGGCTTTATTCATCATCGAACGCGAAATCGTg < 1:2103355/71‑1 (MQ=255) aagaagATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGga < 1:2998829/71‑1 (MQ=255) agaagaTGACGTTTTCCGCCGCCACGCCTTTTTCGATg > 1:886398/1‑38 (MQ=255) agaagaTGACGTTTTCCGCCGCCACGCCTTTTTCGATg > 1:890911/1‑38 (MQ=255) gaagaTGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGga < 1:1622359/69‑1 (MQ=255) aagaTGACGTTTTCCGCCGCCACGCCTTTTTCGATAGCTTTATTCATCATTGAGCGCGAAATCGTGGAGAc > 1:3047927/1‑71 (MQ=255) aagaTGACGTTTTCCGCCGCCACGCCTTTTTCGATAGCTTTATTCATCATTGAGCGCGAAATCGTGGAGAc > 1:1627096/1‑71 (MQ=255) agaTGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTa > 1:1189693/1‑41 (MQ=255) agaTGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACg < 1:210928/71‑1 (MQ=255) aTGACGTTTTCCGCCGCCACGCCTTTTTCGATAGCTTTa < 1:895715/39‑1 (MQ=255) tGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCtt < 1:2399447/36‑1 (MQ=255) gACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTAt > 1:113172/1‑71 (MQ=255) aCGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATc < 1:2772857/71‑1 (MQ=255) cGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCg < 1:1441299/71‑1 (MQ=255) ttCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGt > 1:633918/1‑71 (MQ=255) tCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGt < 1:2427481/70‑1 (MQ=255) cgccgcCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTa < 1:3601231/70‑1 (MQ=255) gccACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAg < 1:1586479/71‑1 (MQ=255) cACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTc < 1:36195/71‑1 (MQ=255) aCGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTcc < 1:2126487/71‑1 (MQ=255) aCGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTcc > 1:1681011/1‑71 (MQ=255) tttttCGATGGCTTTATTCATCATCGAACGCGTAATCGTGGAGACg < 1:3279211/46‑1 (MQ=255) tttttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGga < 1:1040343/42‑1 (MQ=255) tttttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGga < 1:2100174/42‑1 (MQ=255) tttttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGAc < 1:3546108/45‑1 (MQ=255) tttttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGt < 1:947510/56‑1 (MQ=255) tttCGATTGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGtt > 1:349433/1‑46 (MQ=255) tttCGATTGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGtt > 1:2256123/1‑46 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCg > 1:2940538/1‑36 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCg > 1:688488/1‑36 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGa > 1:3014834/1‑51 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTa > 1:2840733/1‑56 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATg < 1:3798252/58‑1 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCa > 1:2987136/1‑60 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCa > 1:1717177/1‑60 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCg > 1:1105452/1‑65 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCg > 1:3200745/1‑65 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACg < 1:671017/70‑1 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACg < 1:246363/70‑1 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGt > 1:3651317/1‑71 (MQ=255) tttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGt > 1:1588828/1‑71 (MQ=255) tttCGACGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGa > 1:497854/1‑51 (MQ=255) ttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGga > 1:583586/1‑39 (MQ=255) ttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGa > 1:2666767/1‑50 (MQ=255) ttCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGa > 1:649948/1‑50 (MQ=255) tCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGtt > 1:2568970/1‑70 (MQ=255) gATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCg < 1:2884425/70‑1 (MQ=255) | AAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGACGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCG > W3110S.gb/2127254‑2127386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |