| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,135,190 | T→A | 6.9% | T115T (ACA→ACT) | wcaA ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,135,190 | 0 | T | A | 6.9% | 188.3 / 4.8 | 72 | T115T (ACA→ACT) | wcaA | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (23/44); new base A (3/2); total (26/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCA > W3110S.gb/2135124‑2135259 | cAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGa > 1:2425311/1‑71 (MQ=255)cAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGa > 1:2961435/1‑71 (MQ=255) aaGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGAc > 1:1329944/1‑71 (MQ=255) aGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGAcc < 1:2086894/71‑1 (MQ=255) aGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGAcc < 1:2381118/71‑1 (MQ=255) aGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGAcc < 1:3165853/71‑1 (MQ=255) aGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGAcc < 1:3810651/71‑1 (MQ=255) gCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCa < 1:881718/71‑1 (MQ=255) gCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCa < 1:1601400/71‑1 (MQ=255) ccGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGc < 1:1056566/71‑1 (MQ=255) ttGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:3179371/71‑1 (MQ=255) ttGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:587598/71‑1 (MQ=255) ttGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:2822031/71‑1 (MQ=255) tGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:1741630/70‑1 (MQ=255) gggAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:2596861/69‑1 (MQ=255) ggAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTAt < 1:3598729/71‑1 (MQ=255) ggAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTAt < 1:1691423/71‑1 (MQ=255) aaTAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:1413761/66‑1 (MQ=255) aaTAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:1205747/66‑1 (MQ=255) aaTAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:1507449/66‑1 (MQ=255) aaTAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCATGTGTGACCAGTTGCTGtt < 1:3312913/66‑1 (MQ=255) aaTAGACTTCGCACTGGCAAACGTAGTCGTTAGCGTACAAAAAGGC‑‑GTGTGACCAGTTGCTGtt < 1:1481309/64‑1 (MQ=255) tAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGc > 1:46591/1‑71 (MQ=255) gACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCgtgt > 1:711380/1‑46 (MQ=255) aCTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAg < 1:1315291/71‑1 (MQ=255) ttCGCCCTGGCAAACGTAGTCGTTGGCGTACAAAAAGGCGTGTGTGAcc < 1:1129520/49‑1 (MQ=255) ttCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCgtgtgt < 1:2417016/45‑1 (MQ=255) ttCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTg < 1:697765/54‑1 (MQ=255) ttCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAgg > 1:969533/1‑70 (MQ=255) cGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGa < 1:3368841/69‑1 (MQ=255) cGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGa < 1:615749/69‑1 (MQ=255) gCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGa > 1:1355289/1‑71 (MQ=255) cTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGc < 1:3840031/49‑1 (MQ=255) cTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGc < 1:338681/49‑1 (MQ=255) tGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGttt < 1:2055785/53‑1 (MQ=255) ggCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTa > 1:2201219/1‑53 (MQ=255) gCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAgg < 1:1058865/61‑1 (MQ=255) gCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCa < 1:1113332/71‑1 (MQ=255) gCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCa < 1:679623/71‑1 (MQ=255) aCGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACg < 1:2194685/71‑1 (MQ=255) cGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGa > 1:1819417/1‑60 (MQ=255) cGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACgg > 1:1485782/1‑71 (MQ=255) gTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGt < 1:2536052/71‑1 (MQ=255) tAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTg < 1:1322025/41‑1 (MQ=255) tAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGttt < 1:1578229/44‑1 (MQ=255) gTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTg < 1:1049570/70‑1 (MQ=255) gTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTg < 1:334401/70‑1 (MQ=255) gTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTg < 1:2982058/70‑1 (MQ=255) gTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTg < 1:2821969/70‑1 (MQ=255) gTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTg < 1:2601216/70‑1 (MQ=255) tCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTggg > 1:139457/1‑71 (MQ=255) tCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTggg > 1:13755/1‑71 (MQ=255) tCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTgg > 1:1631598/1‑70 (MQ=255) cGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGtt < 1:1440080/39‑1 (MQ=255) gTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACgg < 1:445807/64‑1 (MQ=255) ttAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTa > 1:1455684/1‑39 (MQ=255) ttAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTa > 1:679079/1‑39 (MQ=255) ttAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTa > 1:2481801/1‑39 (MQ=255) tAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGgtgt > 1:1080462/1‑70 (MQ=255) aGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTc < 1:299384/70‑1 (MQ=255) aGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTc > 1:3061220/1‑70 (MQ=255) aGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTc > 1:2963329/1‑70 (MQ=255) aGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGACGAGGAAGACGCTCAGACGGTTGGGTGTc > 1:805988/1‑70 (MQ=255) tACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtc > 1:1271341/1‑71 (MQ=255) tACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtc < 1:3221442/71‑1 (MQ=255) cAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:3481438/1‑71 (MQ=255) cAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:1888866/1‑71 (MQ=255) cAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:1929333/1‑71 (MQ=255) cAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcg > 1:468884/1‑70 (MQ=255) aaaaGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtca < 1:2922105/71‑1 (MQ=255) aaaaGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTCCATtcatcg < 1:48072/71‑2 (MQ=255) aaaaGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTCCATtcatcg < 1:2219764/71‑2 (MQ=255) aaGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGt > 1:1553456/1‑52 (MQ=255) aaGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGt > 1:2676393/1‑52 (MQ=255) gtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTcgtcatcgtcat > 1:3818907/1‑69 (MQ=255) tgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCa > 1:3771630/1‑54 (MQ=255) tgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtca < 1:3489899/61‑1 (MQ=255) tgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGtcatca > 1:2102978/1‑70 (MQ=255) | CAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCA > W3110S.gb/2135124‑2135259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |