| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,278,280 | C→T | 11.6% | R256R (CGC→CGT) | yejB → | predicted oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,278,280 | 0 | C | T | 11.6% | 112.1 / 6.0 | 43 | R256R (CGC→CGT) | yejB | predicted oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (14/24); new base T (3/2); total (17/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACATGTGTT > W3110S.gb/2278215‑2278348 | aCAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCgcaagc < 1:2628827/70‑1 (MQ=255) cAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCgcaagcaa < 1:3477699/71‑1 (MQ=255) cAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCgcaagcaa < 1:1099953/71‑1 (MQ=255) aGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCgcaagcaa > 1:3830516/1‑70 (MQ=255) aGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCgcaagcaa > 1:2202761/1‑70 (MQ=255) aTTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACgt < 1:1274841/71‑1 (MQ=255) tggtggCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACgtgg > 1:2569503/1‑71 (MQ=255) gtggCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACgtggt < 1:1191328/70‑1 (MQ=255) tGCGGCTCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGTAAGCAATACGTGGTGACggcac > 1:1743001/1‑69 (MQ=255) tGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACcgcgc > 1:54400/1‑71 (MQ=255) tGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACcgcgc > 1:2074724/1‑71 (MQ=255) tGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACcgc < 1:1834288/69‑1 (MQ=255) gcTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGa < 1:3677959/71‑1 (MQ=255) gcTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGa < 1:3588672/71‑1 (MQ=255) cTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGaa < 1:1999957/71‑1 (MQ=255) tGACCATGCTGACAAAAAACTCATTCCTTGGTGAAGTGCgcaagc < 1:109155/45‑1 (MQ=255) gACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGa > 1:2061874/1‑68 (MQ=255) aTGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAgggg > 1:1258514/1‑70 (MQ=255) tGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTa < 1:2319652/71‑1 (MQ=255) cTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACgtggt < 1:1516480/47‑1 (MQ=255) cTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAg < 1:613759/71‑1 (MQ=255) cTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAg < 1:300608/71‑1 (MQ=255) cTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTCGTGACCGCGCGTGCGAAAGGGGTaa < 1:3712905/70‑1 (MQ=255) aCAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACgtg < 1:1083354/42‑1 (MQ=255) aCAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACgtg < 1:1122917/42‑1 (MQ=255) aaaaaaCTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTaa < 1:553084/65‑1 (MQ=255) aaaaaaCTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTaa < 1:1591475/65‑1 (MQ=255) aaaaaCTCATTCCTTGATGATGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTaa < 1:1550288/64‑1 (MQ=255) aaaaaCTCATTCCTTGATGAAGTGCGTAAGCAATACGTGGTGAc > 1:2575175/1‑44 (MQ=255) aaaaaCTCATTCCTTGATGAAGTGCGTAAGCAATACGTGGTGAc > 1:2159265/1‑44 (MQ=255) aaaaCTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGaaaa > 1:3546767/1‑70 (MQ=255) aaaCTCATTCCTTGATGAAGTGCGTAAGCAATACgtggt < 1:2581585/39‑1 (MQ=255) aaaCTCATTCCTTGATGAAGTGCGTAAGCAATACgtggt < 1:3684508/39‑1 (MQ=255) aaaCTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACcg > 1:3686075/1‑44 (MQ=255) aaaCTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGaaaaa > 1:2934998/1‑70 (MQ=255) aaaCTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGaaaaa > 1:1305355/1‑70 (MQ=255) ccTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAg > 1:3493220/1‑54 (MQ=255) tgatgaAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTgg > 1:1580331/1‑70 (MQ=255) gatgaAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGa < 1:2488198/70‑1 (MQ=255) atgaAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGt > 1:567733/1‑46 (MQ=255) atgaAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGt > 1:995025/1‑46 (MQ=255) atgaAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGaaa < 1:3082935/71‑1 (MQ=255) aaGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTaa > 1:3337994/1‑45 (MQ=255) aaGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACAt < 1:3303533/71‑1 (MQ=255) aaGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACAt < 1:1207487/71‑1 (MQ=255) aaGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACAt < 1:937002/71‑1 (MQ=255) tGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGaaa < 1:106711/65‑1 (MQ=255) tGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACAtgtg < 1:2035899/71‑1 (MQ=255) tGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACAtgtg < 1:919986/71‑1 (MQ=255) gcgcAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACATgtgt < 1:1062915/71‑1 (MQ=255) cgcAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACATgtgt < 1:3403199/70‑1 (MQ=255) cgcAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACATgtgt < 1:3398658/70‑1 (MQ=255) cgcAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACATGTGtt > 1:2018417/1‑71 (MQ=255) | ACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCATTCCTTGATGAAGTGCGCAAGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTAAGTGAAAAAAATATTCTCTGGAAACATGTGTT > W3110S.gb/2278215‑2278348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |