| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 77,316 | T→C | 9.4% | intergenic (‑17/‑305) | sgrR ← / → setA | DNA‑binding transcriptional regulator/broad specificity sugar efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 77,316 | 0 | T | C | 9.4% | 168.3 / 7.2 | 64 | intergenic (‑17/‑305) | sgrR/setA | DNA‑binding transcriptional regulator/broad specificity sugar efflux system |
| Reads supporting (aligned to +/- strand): ref base T (30/28); new base C (3/3); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CGCAGCATTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCC > W3110S.gb/77248‑77386 | cGCAGCATTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCtgtg > 1:1668418/1‑70 (MQ=255) gcagcaTTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGc > 1:1211790/1‑70 (MQ=255) cagcaTTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCa > 1:1483717/1‑70 (MQ=255) gCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAg > 1:1187887/1‑71 (MQ=255) gCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAg > 1:1321159/1‑71 (MQ=255) gCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAatttt < 1:3051053/71‑1 (MQ=255) aTGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAattttattt < 1:1626539/71‑1 (MQ=255) aTGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAattttattt < 1:3750149/71‑1 (MQ=255) tGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAattttatttt < 1:2657074/71‑1 (MQ=255) tGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAattttattt < 1:3420452/70‑1 (MQ=255) gAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTc > 1:2844653/1‑71 (MQ=255) aaCTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTcc > 1:3699353/1‑71 (MQ=255) aaCTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTcc > 1:704786/1‑71 (MQ=255) aCTGTTGTTGCAGACGAGCAGATTGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTccc < 1:3593492/71‑1 (MQ=255) aCTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTccc < 1:3589201/71‑1 (MQ=255) aCTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTccc < 1:3588994/71‑1 (MQ=255) gttgttGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCta > 1:2046473/1‑70 (MQ=255) ttgttgCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCta > 1:868790/1‑69 (MQ=255) tgCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGCGCAAAAGACAGCAattttatt > 1:308863/1‑58 (MQ=255) tgCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGCGCAAAAGACAGCAattttatt > 1:1909276/1‑58 (MQ=255) tgCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGCGCAAAAGACAGCAattttatt > 1:3545389/1‑58 (MQ=255) gCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAattttatt > 1:2775547/1‑57 (MQ=255) gCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAatttta > 1:1474162/1‑55 (MQ=255) gCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAg > 1:3387364/1‑71 (MQ=255) aGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTc < 1:634837/71‑1 (MQ=255) aCGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaa < 1:1678424/71‑1 (MQ=255) cGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaat < 1:1943034/71‑1 (MQ=255) cGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaat < 1:2725898/71‑1 (MQ=255) gATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTaa > 1:3301478/1‑59 (MQ=255) aTGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAattttattt < 1:1323197/46‑1 (MQ=255) gCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCtat > 1:652312/1‑50 (MQ=255) aTAAAAGGGGAACTCCTGTGCAAAAGCCAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGa > 1:933129/1‑71 (MQ=255) aTAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGa > 1:2301213/1‑71 (MQ=255) tAAAAGGGTAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGAt < 1:3837224/71‑1 (MQ=255) tAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTccc < 1:1246333/44‑1 (MQ=255) tAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTccc < 1:3624282/44‑1 (MQ=255) tAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCt < 1:2330371/65‑1 (MQ=255) aaaaGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGAt > 1:510162/1‑70 (MQ=255) aaaGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTaa > 1:1897847/1‑50 (MQ=255) aaaGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTaa > 1:1557627/1‑50 (MQ=255) aaaGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATg > 1:536422/1‑70 (MQ=255) aaaGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATg > 1:3749631/1‑70 (MQ=255) aaaGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATg > 1:1344616/1‑70 (MQ=255) aGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaa > 1:1579110/1‑53 (MQ=255) aGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAg > 1:3337331/1‑71 (MQ=255) aGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAg < 1:749676/71‑1 (MQ=255) ggggAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTatat > 1:2803067/1‑44 (MQ=255) ggggAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTa > 1:848327/1‑46 (MQ=255) gggAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGc < 1:3167586/70‑1 (MQ=255) ggAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCaa < 1:2319362/71‑1 (MQ=255) ggAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCaa < 1:3478351/71‑1 (MQ=255) aaCTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAgg < 1:3185399/71‑1 (MQ=255) aaCTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAgg < 1:3783324/71‑1 (MQ=255) aaCTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAgg < 1:2625911/71‑1 (MQ=255) aCTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAggg < 1:3280073/71‑1 (MQ=255) cTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaat < 1:309601/47‑1 (MQ=255) tCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAggggg > 1:3810813/1‑71 (MQ=255) cTGTGCAAAAGACAGCAATTTTATTTTCCCTatat > 1:2134176/1‑35 (MQ=255) cTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaataa > 1:384289/1‑46 (MQ=255) cTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGc < 1:702463/62‑1 (MQ=255) cTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGc < 1:2420289/62‑1 (MQ=255) cTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTg > 1:2652764/1‑71 (MQ=255) cTGCGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaa < 1:1444831/43‑1 (MQ=255) cTGCGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaa < 1:3519430/43‑1 (MQ=255) cTGCGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaa < 1:111583/43‑1 (MQ=255) tgtgCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGAt < 1:354477/56‑1 (MQ=255) tgCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaataa > 1:2439478/1‑43 (MQ=255) tgCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCaat < 1:21838/41‑1 (MQ=255) tgCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATTAAGCAAGGGGGTGccc > 1:3518789/1‑71 (MQ=255) tgCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGccc < 1:579208/71‑1 (MQ=255) tgCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGccc < 1:3230543/71‑1 (MQ=255) | CGCAGCATTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCC > W3110S.gb/77248‑77386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |