| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,279,035 | G→A | 11.1% | S143S (TCG→TCA) | yejE → | predicted oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,279,035 | 0 | G | A | 11.1% | 119.1 / 5.2 | 45 | S143S (TCG→TCA) | yejE | predicted oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/23); new base A (2/3); total (19/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
GGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGC > W3110S.gb/2278976‑2279104 | ggAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGttt < 1:125164/69‑1 (MQ=255)ggAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTg < 1:1743600/70‑1 (MQ=255) aaCGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGcc < 1:2165190/71‑1 (MQ=255) aaCGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGcc < 1:3722106/71‑1 (MQ=255) aTGCCAATGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCAGTTCTGttt > 1:2485475/1‑62 (MQ=255) aTGCCAATGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCAGTTCTGttt > 1:3360764/1‑62 (MQ=255) aaCGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTg > 1:1524360/1‑63 (MQ=255) gATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTc < 1:2900456/71‑1 (MQ=255) gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGttt > 1:1677736/1‑45 (MQ=255) gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGttt > 1:2035119/1‑45 (MQ=255) gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGtt > 1:3198585/1‑44 (MQ=255) gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGtt > 1:3736845/1‑44 (MQ=255) gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGt > 1:1749342/1‑43 (MQ=255) gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGAt > 1:2939247/1‑53 (MQ=255) cTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGt < 1:2900414/71‑1 (MQ=255) cTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGt < 1:2071699/71‑1 (MQ=255) aCGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTTTTCCAGCGTGa < 1:3787765/68‑1 (MQ=255) ttCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGAt < 1:593017/40‑1 (MQ=255) ttCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCt < 1:424648/43‑1 (MQ=255) ttCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGActctct < 1:1829642/51‑1 (MQ=255) ttCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGa < 1:1394134/63‑1 (MQ=255) ttCTCTATGGCACGCGGATCTCGGTGCTGTTTGGCCTGATGCt < 1:46691/43‑1 (MQ=255) ttCTCTATGGCACGCGGATCTCAGTTCTGTTTGGCCTGATGCTGAcc < 1:2424841/47‑2 (MQ=39) ttCTCTATGGCACGCGGATCTCAGTTCTGTTTGGCCTGATGCTGAcc < 1:445408/47‑2 (MQ=39) ttCTCTATGGCACGCGGATCTCAGTTCTGTTTGGCCTGATGCTGAcc < 1:1581027/47‑2 (MQ=39) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGa > 1:257880/1‑37 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGc > 1:2600719/1‑40 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGc > 1:752155/1‑40 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGc > 1:1418019/1‑40 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGa > 1:3806373/1‑43 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGt > 1:2999963/1‑51 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTcc < 1:2726281/54‑1 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGAGTGa > 1:1313064/1‑61 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGAGTGa > 1:1673915/1‑61 (MQ=255) ctctATGGCACGCAGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGa > 1:2174733/1‑61 (MQ=255) tATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGa > 1:2450554/1‑58 (MQ=255) tATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATgg < 1:895391/61‑1 (MQ=255) tATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCt < 1:583415/68‑1 (MQ=255) tGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGc < 1:3137819/69‑1 (MQ=255) cACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAg < 1:2072233/48‑1 (MQ=255) aCGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGgc < 1:1481081/71‑1 (MQ=255) cgcgGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGgcg < 1:2699324/71‑1 (MQ=255) aTCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGt < 1:2458032/44‑1 (MQ=255) aTCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTAc < 1:1552897/70‑1 (MQ=255) ctcGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTgg < 1:236944/56‑1 (MQ=255) ggTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGa > 1:1570042/1‑41 (MQ=255) ggTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGc > 1:2549340/1‑70 (MQ=255) | GGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGC > W3110S.gb/2278976‑2279104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |