| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,320,580 | G→A | 6.6% | N52N (AAC→AAT) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,320,580 | 0 | G | A | 6.6% | 276.4 / 6.1 | 91 | N52N (AAC→AAT) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base G (39/46); new base A (4/2); total (43/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
CGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCG > W3110S.gb/2320510‑2320650 | cGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATg > 1:3006894/1‑71 (MQ=255)cGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATg > 1:3002256/1‑71 (MQ=255)cGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATg > 1:300787/1‑71 (MQ=255)cGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATAATTGGCATg > 1:3613723/1‑71 (MQ=255) aTTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGtt < 1:2914393/71‑1 (MQ=255) aTTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGtt < 1:563401/71‑1 (MQ=255) aTTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGtt < 1:2433917/71‑1 (MQ=255) ccGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACg < 1:3257805/71‑1 (MQ=255) gCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGtt < 1:1181371/64‑1 (MQ=255) gCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTg < 1:2219334/65‑1 (MQ=255) cAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGtt < 1:1914328/63‑1 (MQ=255) aaCGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCt < 1:3207344/71‑1 (MQ=255) aCGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTa < 1:2047696/71‑1 (MQ=255) aCGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTa < 1:1712593/71‑1 (MQ=255) aCGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTa < 1:1402641/71‑1 (MQ=255) gTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAg > 1:3238409/1‑71 (MQ=255) gTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAg > 1:3598976/1‑71 (MQ=255) tCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGt > 1:3011836/1‑63 (MQ=255) tCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGt > 1:824352/1‑63 (MQ=255) aGTCCCAACTGACGAATGCGTTGCGTCAAGCTGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc > 1:2078163/1‑71 (MQ=255) aGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc > 1:2576933/1‑71 (MQ=255) aGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc < 1:3806067/71‑1 (MQ=255) aGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc < 1:3588095/71‑1 (MQ=255) tCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc < 1:837939/69‑1 (MQ=255) tCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGa < 1:600676/71‑1 (MQ=255) cccAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGa > 1:3520598/1‑70 (MQ=255) cccAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGa > 1:3131045/1‑70 (MQ=255) cccAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGa < 1:3636444/70‑1 (MQ=255) ccAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGata > 1:1942142/1‑71 (MQ=255) caaaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGata > 1:2813521/3‑71 (MQ=255) caaaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGata > 1:2268616/3‑71 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGatat > 1:646001/1‑70 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGatat > 1:1248832/1‑70 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGatat > 1:1871863/1‑70 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGatat > 1:2896424/1‑70 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATc < 1:1770606/71‑1 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATATTGACGTCTCTAAGCACGatat > 1:2248187/1‑70 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATATTGACGTCGCTAAGCACGatat > 1:3578924/1‑70 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATATTGACGTCGCTAAGCACGatat > 1:2632954/1‑70 (MQ=255) aaCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATATTGACGTCGCTAAGCACGatat > 1:257109/1‑70 (MQ=255) aCTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGtt < 1:3689811/49‑1 (MQ=255) cTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc < 1:2637454/63‑1 (MQ=255) cTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc < 1:1918874/63‑1 (MQ=255) cTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc < 1:3729537/63‑1 (MQ=255) cTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCaa < 1:3075429/71‑1 (MQ=255) cTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCaa < 1:340821/71‑1 (MQ=255) cTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCaa > 1:2249295/1‑71 (MQ=255) cTGACGAATGCGTTGAGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCaa < 1:3833985/71‑1 (MQ=255) tGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGa > 1:239055/1‑49 (MQ=255) tGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGa > 1:3285497/1‑49 (MQ=255) gACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGa > 1:2816034/1‑48 (MQ=255) gACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGACATCAata > 1:819005/1‑71 (MQ=255) aCGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAatat > 1:1119624/1‑71 (MQ=255) aaTGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGAt < 1:64850/71‑1 (MQ=255) aTGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCaa > 1:2472436/1‑64 (MQ=255) aTGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCaa > 1:23575/1‑64 (MQ=255) tgcgttgcgtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTc > 1:1574412/1‑71 (MQ=255) gcgttgcgtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCt > 1:1733879/1‑71 (MQ=255) cgttgcgtCAAGCGGTAACCATCCAGATTTGGCATGTTGACGTCGCTaa > 1:2668457/1‑49 (MQ=255) gttgcgtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTg < 1:1174355/71‑1 (MQ=255) ttgcgtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCAc < 1:17585/51‑1 (MQ=255) gcgtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCt < 1:240866/70‑1 (MQ=255) gcgtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCt < 1:353545/70‑1 (MQ=255) cgtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCt < 1:3282913/69‑1 (MQ=255) gtCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAg < 1:3729771/71‑1 (MQ=255) cAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGt > 1:1993918/1‑70 (MQ=255) aaGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTa > 1:1023359/1‑70 (MQ=255) aGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACa < 1:3042343/71‑1 (MQ=255) cGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAtt < 1:1219896/71‑1 (MQ=255) cGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAtt < 1:324870/71‑1 (MQ=255) cGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAt > 1:1997716/1‑70 (MQ=255) cGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAt > 1:3764664/1‑70 (MQ=255) cGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAt > 1:3644035/1‑70 (MQ=255) cGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAt > 1:959345/1‑70 (MQ=255) ggTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTa < 1:1381637/71‑1 (MQ=255) gTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGGTATCAATATGATTCTTGCTAAGTACATTag < 1:321060/71‑2 (MQ=255) gTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTaa < 1:2450783/71‑1 (MQ=255) ccctccatATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAgcgc < 1:3710976/68‑1 (MQ=255) ccatccatATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAgcgc < 1:731629/71‑1 (MQ=255) ccatccatATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAgcgc < 1:1840552/71‑1 (MQ=255) atccatATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCAt > 1:3024498/1‑71 (MQ=255) catATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCa > 1:459530/1‑67 (MQ=255) catATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGa > 1:1918223/1‑71 (MQ=255) atatTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAgcgc < 1:119622/65‑1 (MQ=255) tttGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCtt > 1:2455717/1‑44 (MQ=255) tttGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGc > 1:995447/1‑70 (MQ=255) tttGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGc < 1:1805602/70‑1 (MQ=255) ttGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGc < 1:3386222/69‑1 (MQ=255) tGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCcat > 1:2353824/1‑71 (MQ=255) ggCATGTTGACGTCGCTAAGCACGACATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCcat < 1:1182182/70‑1 (MQ=255) cATATTGACGTCGCTAAGCACGATATCAATATGATTCTTGTTAAGTACATTAAGCGCATCGACGCcatcat < 1:3286474/71‑1 (MQ=255) cATATTGACGTCGCTAAGCACGATATCAATATGATTCTTGTTAAGTACATTAAGCGCATCGACGCcatcat < 1:2598423/71‑1 (MQ=255) tGTTGACTTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTc > 1:615171/1‑71 (MQ=255) tGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTc < 1:2639844/71‑1 (MQ=255) tGTTGACGTCGCT‑AGCACGATATCAATATGATTCTTGCt < 1:1928456/39‑1 (MQ=37) gTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTAc > 1:3754670/1‑45 (MQ=255) gTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTcg > 1:1212432/1‑71 (MQ=255) | CGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCG > W3110S.gb/2320510‑2320650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |