| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,546,874 | G→A | 7.8% | G84G (GGC→GGT) | cysW ← | sulfate/thiosulfate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,546,874 | 0 | G | A | 7.8% | 226.8 / 6.7 | 77 | G84G (GGC→GGT) | cysW | sulfate/thiosulfate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (41/30); new base A (3/3); total (44/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GTCCAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATG > W3110S.gb/2546805‑2546943 | gTCCAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGc > 1:2335265/1‑71 (MQ=255)gTCCAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGc > 1:3663788/1‑71 (MQ=255) ccAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGcc > 1:3573594/1‑70 (MQ=255) gTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGaa > 1:1819887/1‑70 (MQ=255) gTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGaa > 1:2404122/1‑70 (MQ=255) agcagTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGtt > 1:3761254/1‑71 (MQ=255) agcagTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGtt > 1:2713199/1‑71 (MQ=255) agTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTAc < 1:2024923/71‑1 (MQ=255) aaCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAaca > 1:875751/1‑58 (MQ=255) aCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCgg > 1:889416/1‑70 (MQ=255) aCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCgg > 1:1240075/1‑70 (MQ=255) aCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCgg > 1:3320065/1‑70 (MQ=255) aCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCgg > 1:890166/1‑70 (MQ=255) aCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCgg < 1:3391524/70‑1 (MQ=255) aCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCgg > 1:3472623/1‑70 (MQ=255) aCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCgg < 1:25029/70‑1 (MQ=255) cGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGc < 1:1527678/71‑1 (MQ=255) aGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAgg < 1:3442071/52‑1 (MQ=255) aGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAAt > 1:679740/1‑69 (MQ=255) gggAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAgg < 1:1086771/51‑1 (MQ=255) aaGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAgcgc > 1:124116/1‑71 (MQ=255) aGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGcgcg < 1:15275/71‑1 (MQ=255) aGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACAGGTACGGCAATCAGcgcg < 1:3567024/71‑1 (MQ=255) gTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTAcc > 1:3553064/1‑52 (MQ=255) gTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTAcc > 1:1542768/1‑52 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCa < 1:3649665/43‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat < 1:3669467/71‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGa < 1:1039991/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGa < 1:2629025/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGa < 1:761598/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGa < 1:109577/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGa < 1:2475900/70‑1 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGaa > 1:3494911/1‑37 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:780045/1‑70 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:3848421/1‑70 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:1390742/1‑70 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:2570307/1‑70 (MQ=255) aaaGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGatca > 1:77770/1‑71 (MQ=255) aGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGatcatc < 1:1560657/71‑1 (MQ=255) aGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGatcatc < 1:1733100/71‑1 (MQ=255) cgTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCg < 1:3124180/71‑1 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAg > 1:1097660/1‑71 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAg > 1:888332/1‑71 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAg > 1:1597718/1‑71 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACA‑CAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGc > 1:3014459/1‑71 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAAcgcgt < 1:3757762/57‑2 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGATCATCACCGTCAGc > 1:2454369/1‑71 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGATCATCACCGTCAGc > 1:1741100/1‑71 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGATCATCACCGTCAGc > 1:2458646/1‑71 (MQ=255) cagccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGc < 1:221611/70‑1 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATc > 1:1131437/1‑48 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATc > 1:849851/1‑48 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:2196249/1‑56 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:3543267/71‑1 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:2188521/71‑1 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:3660935/71‑1 (MQ=255) gccaggccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAg < 1:3583587/71‑1 (MQ=255) ccaggccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCa > 1:2594532/1‑47 (MQ=255) ccaggccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGa < 1:655220/71‑1 (MQ=255) ccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:3475879/1‑49 (MQ=255) ccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:806121/1‑49 (MQ=255) ccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:2097196/1‑49 (MQ=255) cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCg > 1:1312601/1‑70 (MQ=255) cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCg > 1:1680972/1‑70 (MQ=255) cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGt > 1:442948/1‑71 (MQ=255) agcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTg < 1:1641828/71‑1 (MQ=255) agcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTg < 1:3165363/71‑1 (MQ=255) gcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCg < 1:2712367/54‑1 (MQ=255) gcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCg < 1:2270951/54‑1 (MQ=255) cagAATGCCGAACACAAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgca < 1:992280/71‑1 (MQ=255) agAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag > 1:1062696/1‑71 (MQ=255) gAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcagc < 1:1125755/71‑1 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg > 1:536696/1‑71 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg < 1:2612335/71‑1 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg > 1:2206729/1‑71 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg > 1:1554308/1‑71 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg < 1:1007031/71‑1 (MQ=255) | GTCCAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATG > W3110S.gb/2546805‑2546943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |