| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,546,906 | G→A | 7.5% | L74L (CTG→TTG) | cysW ← | sulfate/thiosulfate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,546,906 | 0 | G | A | 7.5% | 239.8 / 5.6 | 80 | L74L (CTG→TTG) | cysW | sulfate/thiosulfate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (39/35); new base A (3/3); total (42/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATC > W3110S.gb/2546839‑2546976 | aaGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAgcgc > 1:124116/1‑71 (MQ=255) aGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGcgcg < 1:15275/71‑1 (MQ=255) aGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACAGGTACGGCAATCAGcgcg < 1:3567024/71‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat < 1:3669467/71‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGa < 1:2629025/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGa < 1:1039991/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGa < 1:761598/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGa < 1:109577/70‑1 (MQ=255) ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGa < 1:2475900/70‑1 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:3848421/1‑70 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:2570307/1‑70 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:1390742/1‑70 (MQ=255) tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:780045/1‑70 (MQ=255) aaaGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGatca > 1:77770/1‑71 (MQ=255) aGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGatcatc < 1:1733100/71‑1 (MQ=255) aGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGatcatc < 1:1560657/71‑1 (MQ=255) cgTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCg < 1:3124180/71‑1 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAg > 1:1597718/1‑71 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAg > 1:888332/1‑71 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAg > 1:1097660/1‑71 (MQ=255) aCCAGCCAGGCCAGCAGAATGCCGAACA‑CAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGc > 1:3014459/1‑71 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAAcgcgt < 1:3757762/57‑2 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGATCATCACCGTCAGc > 1:2454369/1‑71 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGATCATCACCGTCAGc > 1:1741100/1‑71 (MQ=255) ccagccagGCCAGCAGAATACCGAACACCAGGTTTACCGGTACGGCAATCAACGCGATCATCACCGTCAGc > 1:2458646/1‑71 (MQ=255) cagccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGc < 1:221611/70‑1 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:2196249/1‑56 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:3660935/71‑1 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:3543267/71‑1 (MQ=255) agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:2188521/71‑1 (MQ=255) gccaggccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAg < 1:3583587/71‑1 (MQ=255) ccaggccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGa < 1:655220/71‑1 (MQ=255) ccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:2097196/1‑49 (MQ=255) ccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:3475879/1‑49 (MQ=255) ccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGat > 1:806121/1‑49 (MQ=255) cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCg > 1:1680972/1‑70 (MQ=255) cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCg > 1:1312601/1‑70 (MQ=255) cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGt > 1:442948/1‑71 (MQ=255) agcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTg < 1:3165363/71‑1 (MQ=255) agcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTg < 1:1641828/71‑1 (MQ=255) gcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCg < 1:2712367/54‑1 (MQ=255) gcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCg < 1:2270951/54‑1 (MQ=255) cagAATGCCGAACACAAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgca < 1:992280/71‑1 (MQ=255) agAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag > 1:1062696/1‑71 (MQ=255) gAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcagc < 1:1125755/71‑1 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg > 1:536696/1‑71 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg < 1:2612335/71‑1 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg > 1:1554308/1‑71 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg > 1:2206729/1‑71 (MQ=255) tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATg < 1:1007031/71‑1 (MQ=255) ccGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTc < 1:3372586/71‑1 (MQ=255) cGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTcc > 1:447794/1‑71 (MQ=255) acacCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGa > 1:969530/1‑71 (MQ=255) acacCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGa < 1:2627423/71‑1 (MQ=255) aGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:254697/44‑1 (MQ=255) aGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGc > 1:289965/1‑71 (MQ=255) aGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGc > 1:89773/1‑71 (MQ=255) aGGTTTACCGGTACGGCAATCAGCGCCATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGc > 1:2914345/1‑71 (MQ=255) ggTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGc > 1:2558348/1‑70 (MQ=255) gTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCa < 1:2250643/42‑1 (MQ=255) gTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAg < 1:1646531/43‑1 (MQ=255) gTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCa < 1:3385845/71‑1 (MQ=255) ttACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGa > 1:3623669/1‑42 (MQ=255) ttACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGa > 1:3157198/1‑42 (MQ=255) ttACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGc > 1:670471/1‑67 (MQ=255) aCCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGAt < 1:1468105/70‑1 (MQ=255) gTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag < 1:2591047/46‑1 (MQ=255) gTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag < 1:20795/46‑1 (MQ=255) gTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag < 1:2682057/46‑1 (MQ=255) gTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag < 1:2633597/46‑1 (MQ=255) cGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCtt > 1:836174/1‑66 (MQ=255) cGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTaa > 1:1031383/1‑70 (MQ=255) cGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTaa > 1:134538/1‑70 (MQ=255) cGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTaa > 1:1440986/1‑70 (MQ=255) cGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTa > 1:3804913/1‑69 (MQ=255) cAATCAGCGCGATCATCACCGTCAGCCAGATGGCGAGCAGCATGTCCGGAACGGCCAGATTCTGTaaaa > 1:2925497/1‑69 (MQ=255) aGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCa < 1:1880751/70‑1 (MQ=255) aGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCAt > 1:30288/1‑71 (MQ=255) aGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCAt > 1:1980460/1‑71 (MQ=255) aGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCAt > 1:1139816/1‑71 (MQ=255) aGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCAt > 1:34846/1‑71 (MQ=255) gcgcGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATc < 1:1651129/71‑1 (MQ=255) | AAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATC > W3110S.gb/2546839‑2546976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |