Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,580,774	T→C	7.3%	P220P (CCA→CCG)	ypfG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,580,774	0	T	C	7.3%	181.0 / 5.0	68	P220P (CCA→CCG)	ypfG	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base C (2/3);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

ACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCG  >  W3110S.gb/2580711‑2580843                                                                |                                                                      acaGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGAt                                                                   >  1:2126337/1‑68 (MQ=255) acaGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATc                                                                  >  1:3338845/1‑69 (MQ=255) acaGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATc                                                                  >  1:3804408/1‑69 (MQ=255)    gCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCg                                                             <  1:367378/71‑1 (MQ=255)    gCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCg                                                             <  1:1433435/71‑1 (MQ=255)     cTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGc                                                              >  1:871774/1‑69 (MQ=255)        atcatcatcAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGag                                                           <  1:3716262/69‑1 (MQ=255)         tcatcatcaGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGag                                                           <  1:3496515/68‑1 (MQ=255)           atcatcaGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAgcg                                                      >  1:428378/1‑71 (MQ=255)              atcaGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAgcg                                                      >  1:3028831/1‑68 (MQ=255)              atcaGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCa                                                    <  1:2550042/70‑1 (MQ=255)              atcaGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCa                                                    <  1:738792/70‑1 (MQ=255)               tcaGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAg                                                   <  1:604269/70‑1 (MQ=255)                 aGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAAt                                                                       >  1:5735/1‑48 (MQ=255)                   cgcTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCAt                                              >  1:1569593/1‑71 (MQ=255)                   cgcTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCAt                                              <  1:2110126/71‑1 (MQ=255)                       ttATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCa                                           <  1:2016139/70‑1 (MQ=255)                        tATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCa                                               >  1:2682296/1‑65 (MQ=255)                        tATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCa                                               >  1:2773608/1‑65 (MQ=255)                        tATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCa                                               >  1:2851003/1‑65 (MQ=255)                        tATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCa                                               >  1:2274189/1‑65 (MQ=255)                        tATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCa                                           <  1:1585121/69‑1 (MQ=255)                        tATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATc                                         >  1:1984104/1‑71 (MQ=255)                        tATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATc                                         >  1:2672749/1‑71 (MQ=255)                            atcaGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGcc                                     <  1:2305219/71‑1 (MQ=255)                            atcaGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGc                                      <  1:3847137/70‑1 (MQ=255)                              cggtcagCGCAGTGACATTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAg                                   >  1:249505/3‑71 (MQ=255)                              cggtcagCGCAGTGACATTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAg                                   >  1:3171396/3‑71 (MQ=255)                               agtcagCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGt                                  >  1:3265521/1‑71 (MQ=255)                               agtcagCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGt                                  >  1:1145971/1‑71 (MQ=255)                               agtcagCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGt                                  >  1:2541170/1‑71 (MQ=255)                                   agCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTc                               >  1:2720494/1‑70 (MQ=255)                                     cgcAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCa                             >  1:744315/1‑70 (MQ=255)                                      gcAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGAcc                                                >  1:3031091/1‑50 (MQ=255)                                       cAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTc                                            <  1:1932723/53‑1 (MQ=255)                                       cAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTc                                            <  1:384118/53‑1 (MQ=255)                                         gTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATc                        <  1:1533967/71‑1 (MQ=255)                                            aCATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAg                     >  1:704792/1‑71 (MQ=255)                                              aTTCACATCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCa                   <  1:2896136/71‑1 (MQ=255)                                                tCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCa                                               >  1:2302941/1‑41 (MQ=255)                                                tCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCa                                               >  1:3541584/1‑41 (MQ=255)                                                   ccTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCg              <  1:1148251/71‑1 (MQ=255)                                                   ccTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCg              <  1:3187915/71‑1 (MQ=255)                                                   ccTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCaaa                 <  1:444698/68‑1 (MQ=255)                                                   ccTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCaaa                 <  1:2956584/68‑1 (MQ=255)                                                   ccTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCaaa                 <  1:2180877/68‑1 (MQ=255)                                                   ccTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCaaa                 <  1:1049669/68‑1 (MQ=255)                                                    cTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCg              <  1:3175516/70‑1 (MQ=255)                                                     taacgacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATc               >  1:2001764/3‑68 (MQ=255)                                                     tCACGACGCAATGGATCAAGCGAGCAGCGCAGACCAt                                              <  1:1281671/37‑1 (MQ=255)                                                     tCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGtt                                 <  1:2325091/50‑1 (MQ=255)                                                     tCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGtt                                 <  1:1990421/50‑1 (MQ=255)                                                     tCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGtt                                 <  1:444001/50‑1 (MQ=255)                                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCAt                                          >  1:771501/1‑40 (MQ=255)                                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATc                        >  1:2632876/1‑58 (MQ=255)                                                       acgacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCg                                       >  1:3568807/1‑42 (MQ=255)                                                       acgacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAg                     >  1:884937/1‑60 (MQ=255)                                                       acgacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGtt            <  1:710860/69‑1 (MQ=255)                                                       acgacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGtt            >  1:815199/1‑69 (MQ=255)                                                       aagacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCAGCCAGTTTCCATAATCCAGCAAATCGtt            >  1:1081997/3‑69 (MQ=255)                                                        cgacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCg         <  1:3647472/71‑1 (MQ=255)                                                         gacgCAATGGATCAAGCGAGCAGCGCAGACCATTc                                            >  1:2500826/1‑35 (MQ=255)                                                         gacgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGt        <  1:1551457/71‑1 (MQ=255)                                                           cgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGttc      >  1:2942855/1‑71 (MQ=255)                                                           cgCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGttc      >  1:2846295/1‑71 (MQ=255)                                                              aaTGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGttcttc   <  1:69005/71‑1 (MQ=255)                                                              aaTGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGttcttc   >  1:228366/1‑71 (MQ=255)                                                              aaTGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGttcttc   >  1:2066451/1‑71 (MQ=255)                                                               aTGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCg  <  1:3753470/71‑1 (MQ=255)                                                                tGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGttcttc   >  1:2813790/1‑69 (MQ=255)                                                                tGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGttcttc   >  1:1736538/1‑69 (MQ=255)                                                                |                                                                      ACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCG  >  W3110S.gb/2580711‑2580843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑