| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 120,479 | G→A | 10.5% | A358V (GCT→GTT) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 120,479 | 0 | G | A | 10.5% | 153.8 / 7.6 | 57 | A358V (GCT→GTT) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (29/22); new base A (2/4); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
CGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACACCAGAATGG > W3110S.gb/120412‑120545 | cgcCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCg < 1:775876/70‑1 (MQ=255)cgcCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCg < 1:1676348/70‑1 (MQ=255) gcCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGa < 1:3562820/71‑1 (MQ=255) aaTCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTgggg > 1:2075254/1‑71 (MQ=255) atcGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAg > 1:3103113/1‑71 (MQ=255) atcGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAg > 1:2942604/1‑71 (MQ=255) atcGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAg > 1:2322084/1‑71 (MQ=255) atcGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAg > 1:1774488/1‑71 (MQ=255) cccAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAg < 1:2304229/71‑1 (MQ=255) aGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGt < 1:464805/66‑1 (MQ=255) aGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTa < 1:1288954/71‑1 (MQ=255) gTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGc < 1:2139925/60‑1 (MQ=255) gATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAg > 1:1877899/1‑60 (MQ=255) aTTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAg < 1:1374351/71‑1 (MQ=255) aTTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAg < 1:3672495/71‑1 (MQ=255) aCCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTAc > 1:3614162/1‑71 (MQ=255) cAGATACCACCAGCGCCATTAACAGTCCGAAAGCg > 1:3332038/1‑35 (MQ=255) cAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTg > 1:3634795/1‑42 (MQ=255) cAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTg > 1:3639269/1‑42 (MQ=255) cAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTg > 1:2713894/1‑42 (MQ=255) cAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAAc > 1:1708973/1‑71 (MQ=255) cAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAAc > 1:1072160/1‑71 (MQ=255) cAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAAc > 1:1803277/1‑71 (MQ=255) gATACCACCAGCGCCATTAACAGTCCGTAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGc < 1:1303808/64‑1 (MQ=255) gATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCTAGGTAGTTAATCAGTACGc < 1:70697/64‑1 (MQ=255) gATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGc < 1:1525183/64‑1 (MQ=255) aTACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGcc < 1:1615065/71‑1 (MQ=255) tACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCa > 1:2766084/1‑56 (MQ=255) tACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCa > 1:794101/1‑56 (MQ=255) tACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCg > 1:597702/1‑71 (MQ=255) accaccAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACaa > 1:2932782/1‑65 (MQ=255) accacaAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACaa > 1:2416068/1‑65 (MQ=255) ccaGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTAcc < 1:3066508/71‑1 (MQ=255) caGCTCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcac < 1:1888646/58‑1 (MQ=255) caGCGCCATTAACAGTCCGAAAACGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTAc > 1:289743/1‑69 (MQ=255) caGCGCCATTAACAGTCCGAAAACGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTAc > 1:3011736/1‑69 (MQ=255) cgcCATTAACAGTCCGAAAGCGGACTATGGGGCAAgg < 1:3777934/37‑1 (MQ=255) ccATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcac < 1:1360420/53‑1 (MQ=255) ccATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcac < 1:551948/53‑1 (MQ=255) ccATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcac < 1:3656243/53‑1 (MQ=255) ttAACAGTCCGAAAACGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGac < 1:571710/71‑1 (MQ=255) ttAACAGTCCGAAAACGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGac < 1:177045/71‑1 (MQ=255) ttAACAGTCCGAAAACGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGac < 1:2562808/71‑1 (MQ=255) tAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAg < 1:3713120/42‑1 (MQ=255) tAACAGTCCGAAAACGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGac < 1:3611648/70‑1 (MQ=255) aaCAGTCCGAAAGCGGACTCTGGGTCAAGGTAGTTAATCAGTACTCACAACGcc < 1:556724/54‑1 (MQ=255) aaCAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCa > 1:378599/1‑40 (MQ=255) aaCAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGc > 1:3770419/1‑53 (MQ=255) aaCAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGc > 1:1470897/1‑53 (MQ=255) aaCAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGc > 1:883178/1‑53 (MQ=255) aaCAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGacac > 1:1389852/1‑71 (MQ=255) aGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGa > 1:648451/1‑65 (MQ=255) cGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCa > 1:3559696/1‑59 (MQ=255) cGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCa > 1:2809515/1‑59 (MQ=255) gAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACACCAGAATgg < 1:3803904/71‑1 (MQ=255) aaaGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACAcc > 1:3812380/1‑63 (MQ=255) aaaGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACACCAGAAt > 1:1670899/1‑68 (MQ=255) | CGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACACCAGAATGG > W3110S.gb/120412‑120545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |