| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,674,927 | C→T | 21.4% | L185L (TTG→TTA) | yphD ← | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,674,927 | 0 | C | T | 21.4% | 51.1 / 9.3 | 28 | L185L (TTG→TTA) | yphD | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (9/13); new base T (3/3); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
ATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGT > W3110S.gb/2674858‑2674997 | aTTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAaaca < 1:2422622/71‑1 (MQ=255) ttACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAaacaa > 1:1674256/1‑71 (MQ=255) aCCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAaacaac > 1:1776046/1‑70 (MQ=255) aaCGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCa < 1:3569129/71‑1 (MQ=255) aCGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCAt < 1:1596184/71‑1 (MQ=255) cGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATg < 1:131084/71‑1 (MQ=255) cGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATg < 1:1644024/71‑1 (MQ=255) ggCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGa < 1:1458651/71‑1 (MQ=255) ggCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGa > 1:2296114/1‑71 (MQ=255) aaaaCCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAg > 1:3456203/1‑71 (MQ=255) aGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCa > 1:828250/1‑57 (MQ=255) tttGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCgaga > 1:84965/1‑70 (MQ=255) ttGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGaa < 1:1390091/70‑1 (MQ=255) ggCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGt < 1:1989850/71‑1 (MQ=255) tgaACACAAACAGCGCAAACAACACAATCATGATCAgcg > 1:1244257/1‑39 (MQ=255) tgaACACAAACAGCGCAAACAACACAATCATGATCAGcgcg < 1:2203970/41‑1 (MQ=255) gaACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGcc > 1:2171302/1‑69 (MQ=255) gaACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGcc > 1:2509680/1‑69 (MQ=255) aaCACAAACAGCGCAAATAACACCATCATGATCAGCGCGGATACCTGCACACCGAGAAATTGTCCGCcca < 1:3446039/70‑1 (MQ=255) aaCACAAACAGCGCAAATAACACCATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCcca < 1:3822183/70‑1 (MQ=255) aaCACAAACAGCGCAAATAACACCATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCcca < 1:2879391/70‑1 (MQ=255) aaCACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCccag > 1:1805618/1‑71 (MQ=255) acacAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCccagc < 1:1790706/71‑1 (MQ=255) acaAACAGCGCAAACAACACAATCATGATCAGcgcg < 1:877828/36‑1 (MQ=255) acaAACAGCGCAAACAACACAATCATGATCAGcgcg < 1:2655282/36‑1 (MQ=255) aCAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTc > 1:2920783/1‑57 (MQ=255) aaaTAACACCATCATGATCAGCGCGGATACCGGCATACCGAGAAATTGTCCGCCCAGCCAATcc > 1:2059209/1‑64 (MQ=255) aaaTAACACCATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAATcc > 1:143473/1‑64 (MQ=255) aaaTAACACCATCATGATCAGCGCGGATACCGGAACACCGAGAAATTGTCCGCCCAGCCAATcc > 1:1418694/1‑64 (MQ=255) aaaCAACACAATCATGATCAGCGCGGATACCGGCACACCGAGTAATTg < 1:3519471/48‑1 (MQ=255) aaaCAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCcca < 1:3102672/56‑1 (MQ=255) acaacaCAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGccc > 1:3322815/1‑53 (MQ=255) acaacaCAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGccc > 1:3379924/1‑53 (MQ=255) acaacaCAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGccc > 1:1102683/1‑53 (MQ=255) caacaCAATCATGATCAGCGCGGATACCGGCACACCTAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGt < 1:3037218/71‑1 (MQ=255) caacaCAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGt < 1:3632623/71‑1 (MQ=255) | ATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGT > W3110S.gb/2674858‑2674997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |