| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,677,646 | C→A | 24.9% | G126G (GGG→GGT) | yphF ← | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,677,646 | 0 | C | A | 24.9% | 48.8 / 6.7 | 24 | G126G (GGG→GGT) | yphF | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (8/10); new base A (4/2); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
CGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGAC > W3110S.gb/2677579‑2677707 | cGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGAccccc < 1:483283/71‑1 (MQ=255)cGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGAccccc < 1:2005060/71‑1 (MQ=255) cccAGTTTTTTACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCtttt < 1:730347/70‑1 (MQ=255) cAGTTTTTTACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGAt > 1:948760/1‑71 (MQ=255) aGTTTTTTACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCtttt < 1:2085504/67‑1 (MQ=255) ttttttACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTaa < 1:381231/71‑1 (MQ=255) aaTTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGtgtt > 1:818382/1‑71 (MQ=255) ccAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGa > 1:3787499/1‑53 (MQ=255) ccGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCgg < 1:3823130/71‑1 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:1329517/1‑71 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:259059/1‑71 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:1817159/1‑71 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:1353477/1‑71 (MQ=255) gATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAg < 1:1638914/44‑1 (MQ=255) aTACGCCGAGACATATTTATCGACCCCCTTTTGATTAATAc < 1:2928694/41‑1 (MQ=255) aTACGCCGAGACATATTTATCGACCCCCTTTTGATTAATAc < 1:3207217/41‑1 (MQ=255) aCGCCGAGACATATTTATCGACCCCCTTTTGATTAATa > 1:3680779/1‑38 (MQ=255) aCGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATg > 1:3558463/1‑65 (MQ=255) cGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCtt < 1:122814/71‑1 (MQ=255) gagaCATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACt < 1:3130190/71‑1 (MQ=255) atatTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAATGCCCGCCTCACTGgcgc < 1:3152600/71‑1 (MQ=255) atatTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAATGCCCGCCTCACTGgcgc < 1:2385326/71‑1 (MQ=255) tatTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGgcgc < 1:305613/70‑1 (MQ=255) tttATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCg > 1:2434068/1‑56 (MQ=255) tttATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCg > 1:2238240/1‑56 (MQ=255) tttATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGcgac > 1:2759804/1‑71 (MQ=255) | CGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGCCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGAC > W3110S.gb/2677579‑2677707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |