| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,677,682 | C→T | 15.1% | P114P (CCG→CCA) | yphF ← | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,677,682 | 0 | C | T | 15.1% | 92.0 / 8.3 | 40 | P114P (CCG→CCA) | yphF | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (18/15); new base T (4/2); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCAGACACTGCCGATA > W3110S.gb/2677614‑2677746 | ccGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCgg < 1:3823130/71‑1 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:259059/1‑71 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:1329517/1‑71 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:1353477/1‑71 (MQ=255) aCCAGATATGCCGAGACATATTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAAt > 1:1817159/1‑71 (MQ=255) aCGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATg > 1:3558463/1‑65 (MQ=255) cGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCtt < 1:122814/71‑1 (MQ=255) gagaCATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACt < 1:3130190/71‑1 (MQ=255) atatTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAATGCCCGCCTCACTGgcgc < 1:2385326/71‑1 (MQ=255) atatTTATCGACACCCTTTTGATTAATACAGGTGTTGTAGCAAATCACTGGAATGCCCGCCTCACTGgcgc < 1:3152600/71‑1 (MQ=255) tatTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGgcgc < 1:305613/70‑1 (MQ=255) tttATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCg > 1:2434068/1‑56 (MQ=255) tttATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCg > 1:2238240/1‑56 (MQ=255) tttATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGcgac > 1:2759804/1‑71 (MQ=255) cTTTTGATTAATACAGGTGTTGTAGCAAATCACCGTAATGCCCGCTTCACTGGCGCGACGAACgg < 1:2446128/65‑1 (MQ=255) cTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAGTGCCCGCTTCACTGGCGCGACGAACgg < 1:1020862/65‑1 (MQ=255) tttGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTa > 1:2117767/1‑71 (MQ=255) ttGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCt < 1:2969339/69‑1 (MQ=255) tGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACt > 1:891118/1‑71 (MQ=255) ttAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCt < 1:965232/65‑1 (MQ=255) aTACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGcgacga < 1:3068550/51‑1 (MQ=255) aTACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGcgacga < 1:599450/51‑1 (MQ=255) aTACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGcgacga < 1:1202533/51‑1 (MQ=255) aCAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCAtttt > 1:3230340/1‑71 (MQ=255) cAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACt > 1:621582/1‑38 (MQ=255) cAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACt > 1:1000928/1‑38 (MQ=255) cAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCAtttt > 1:1187565/1‑70 (MQ=255) tgttgtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCAGac < 1:839260/71‑1 (MQ=255) ttgtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCt < 1:1004759/53‑1 (MQ=255) ttgtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCAtttt < 1:21051/64‑1 (MQ=255) ttgtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCAGaca < 1:3226090/70‑1 (MQ=255) gtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGt > 1:1397556/1‑45 (MQ=255) gtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTa > 1:99379/1‑46 (MQ=255) gtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCa > 1:3355452/1‑64 (MQ=255) gtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCa > 1:2081229/1‑64 (MQ=255) gtAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCAGACACTg > 1:436027/1‑71 (MQ=255) gtAGCAAATCACAGGAATGCCCGCTTCACTGGCGCGACGAACGGTa > 1:2842479/1‑46 (MQ=255) gCAAATGACCGGAATGCCAGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCAGACACTGcc > 1:3626/1‑70 (MQ=255) aTCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCAGACACTGCCGATa > 1:2269192/1‑70 (MQ=255) acACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCAt > 1:2597642/2‑51 (MQ=255) | CCGACCAGATACGCCGAGACATATTTATCGACCCCCTTTTGATTAATACAGGTGTTGTAGCAAATCACCGGAATGCCCGCTTCACTGGCGCGACGAACGGTACGGCTACTGCCATTTTCAGACACTGCCGATA > W3110S.gb/2677614‑2677746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |