| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,717,266 | C→T | 20.5% | intergenic (‑82/‑125) | yfiF ← / → trxC | predicted methyltransferase/thioredoxin 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,717,266 | 0 | C | T | 20.5% | 77.6 / 14.2 | 39 | intergenic (‑82/‑125) | yfiF/trxC | predicted methyltransferase/thioredoxin 2 |
| Reads supporting (aligned to +/- strand): ref base C (23/8); new base T (2/6); total (25/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTA > W3110S.gb/2717208‑2717336 | gAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAcc > 1:725693/1‑71 (MQ=255)gAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAcc > 1:588916/1‑71 (MQ=255) cgcAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTTCCAAAGCCTGTGTCTATCATACCCATTg > 1:2970235/1‑70 (MQ=255) cgcAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTTCCAAAGCCTGTGTCTATCATACCCATTg > 1:176619/1‑70 (MQ=255) gcAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa > 1:3313621/1‑71 (MQ=255) gATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa < 1:3417639/71‑1 (MQ=255) aTTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt < 1:1165964/54‑1 (MQ=255) aTTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt < 1:3556051/54‑1 (MQ=255) ttACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa > 1:2183619/1‑71 (MQ=255) aCCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa > 1:497999/1‑69 (MQ=255) aCCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa > 1:425331/1‑69 (MQ=255) cccGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc < 1:3714421/44‑1 (MQ=255) cccGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc < 1:1525219/44‑1 (MQ=255) cgcgcgGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTc > 1:3353248/1‑71 (MQ=255) gcgcgGTTCGCCATGATTTCGTTCCAAAGCCTGTGTCTATCATACCCATTGAATAAAAc < 1:2244639/59‑1 (MQ=255) gcgcgGTTCGCCATGATTTCGTTCCAAAGCCTGTGTCTATCATACCCATTGAATAAAAc < 1:631071/59‑1 (MQ=255) cgGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATtgttgt > 1:1597035/1‑66 (MQ=255) ggTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGaa < 1:2019507/71‑1 (MQ=255) ttCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACa < 1:439341/71‑1 (MQ=255) aTGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa > 1:3532970/1‑41 (MQ=255) aTGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACa > 1:97114/1‑48 (MQ=255) aTGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACa > 1:1533706/1‑48 (MQ=255) aTGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcc > 1:2273076/1‑71 (MQ=255) aTGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcc > 1:588687/1‑71 (MQ=255) gATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTccac > 1:1914775/1‑69 (MQ=255) aTTTCGTTCCAAAGCCTGTGTCTATCATACCCATTGAATAAAACAGAttg < 1:2056600/50‑1 (MQ=255) aTTTCGTTCCAAAGCCTGTGTCTATCATACCCATTGAATAAAACAGAttg < 1:2868099/50‑1 (MQ=255) aTTTCGTTCCAAAGCCTGTGTCTATCATACCCATTGAATAAAACAGAttg < 1:615168/50‑1 (MQ=255) aTTTCGTTCCAAAGCCTGTGTCTATCATACCCATTGAATAAAACAGAttg < 1:2504746/50‑1 (MQ=255) aTTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcccc > 1:2878066/1‑70 (MQ=255) aTTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcccc > 1:2567764/1‑70 (MQ=255) tttCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGa > 1:3374073/1‑71 (MQ=255) cGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGata < 1:478021/70‑1 (MQ=255) aCCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAatat > 1:431207/1‑71 (MQ=255) ccAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAatat < 1:2292228/70‑1 (MQ=255) ccAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAatat < 1:3356166/70‑1 (MQ=255) cAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATtgttgta > 1:2036192/1‑45 (MQ=255) cAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcccc > 1:1235056/1‑61 (MQ=255) aaGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTaa > 1:3149916/1‑71 (MQ=255) gCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACa > 1:3629548/1‑71 (MQ=255) gCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACatat > 1:2774132/1‑70 (MQ=255) cGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACatat < 1:353594/69‑1 (MQ=255) cGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACatat < 1:1320077/69‑1 (MQ=255) cGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTa < 1:3837092/71‑1 (MQ=255) | GAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTA > W3110S.gb/2717208‑2717336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |