| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,721,287 | T→C | 5.8% | intergenic (+18/‑96) | yfiQ → / → pssA | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit/phosphatidylserine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,721,287 | 0 | T | C | 5.8% | 187.8 / 2.3 | 69 | intergenic (+18/‑96) | yfiQ/pssA | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit/phosphatidylserine synthase |
| Reads supporting (aligned to +/- strand): ref base T (29/36); new base C (2/2); total (31/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
GAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTC > W3110S.gb/2721219‑2721356 | gagGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGtt > 1:455392/1‑70 (MQ=255) gTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTa < 1:2990692/70‑1 (MQ=255) ttGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAAt < 1:3853395/71‑1 (MQ=255) ttGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAAt < 1:3667687/71‑1 (MQ=255) ggCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGAGCACTTAATCgg < 1:3358814/71‑1 (MQ=255) ggCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCgg < 1:3076846/71‑1 (MQ=255) ggCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCgg < 1:3415828/71‑1 (MQ=255) ggCTTACGCTAAATCTTGCCCAGCGCGAGGAACCATGAGTAAGGTACTGGAAATGTTGACCACTTAATctg < 1:2668910/71‑3 (MQ=255) ttACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGt > 1:197875/1‑53 (MQ=255) aCGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGa > 1:3689072/1‑54 (MQ=255) aCGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGAc < 1:1611314/69‑1 (MQ=255) cGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGtt < 1:2712750/51‑1 (MQ=255) tAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAAt > 1:1453682/1‑59 (MQ=255) tAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAAt > 1:273391/1‑59 (MQ=255) tAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGgtgt > 1:1665012/1‑71 (MQ=255) tAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGgtgt > 1:1909947/1‑71 (MQ=255) tAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGgtgt > 1:1989498/1‑71 (MQ=255) aaTCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGa > 1:2078896/1‑48 (MQ=255) aaTCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGtta > 1:3098005/1‑71 (MQ=255) tCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCgg < 1:1080620/58‑1 (MQ=255) ttGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGa > 1:2971607/1‑44 (MQ=255) ttGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGa > 1:1807335/1‑58 (MQ=255) ttGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTtattat > 1:2897352/1‑71 (MQ=255) ttGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTtattat > 1:2254195/1‑71 (MQ=255) gCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATAGGGACTGgtgt > 1:1635915/1‑63 (MQ=255) ccAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGcc > 1:3480970/1‑71 (MQ=255) aGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGccc > 1:2407345/1‑70 (MQ=255) gcgcGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCgg < 1:565380/49‑1 (MQ=255) cgcgAGGATTCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTTGTGTTATTATTGCCCGCt < 1:2985406/71‑1 (MQ=255) cgcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:2799822/71‑1 (MQ=255) cgcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:444471/71‑1 (MQ=255) cgcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:1157554/71‑1 (MQ=255) cgcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:2626628/71‑1 (MQ=255) cgcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:1703098/71‑1 (MQ=255) cgcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:2419149/71‑1 (MQ=255) cgcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:1976297/71‑1 (MQ=255) gcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCTGGACTGGTGTTATTATTGCCCGCt < 1:1673723/70‑1 (MQ=255) gcgAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:3742848/70‑1 (MQ=255) gAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTAt > 1:128340/1‑71 (MQ=255) gAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTAt > 1:311914/1‑71 (MQ=255) aGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATg < 1:2394341/71‑1 (MQ=255) ggAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCgg < 1:3247752/43‑1 (MQ=255) ggAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATgt < 1:3535695/71‑1 (MQ=255) gAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATgtc < 1:837501/71‑1 (MQ=255) gAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATgtc < 1:264170/71‑1 (MQ=255) aaTCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGttatt < 1:2139416/54‑1 (MQ=255) aaTCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGttatt < 1:3262464/54‑1 (MQ=255) aTCATGAGTAAGGTAATGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATgtcgt > 1:1505910/1‑71 (MQ=255) tCATGAGTAAGGTACTGGAAATGTTGACCACTTAATc < 1:2430991/37‑1 (MQ=255) tCATGAGTAAGGTACTGGAAATGCTGACCACTTAATCgg < 1:547175/39‑1 (MQ=255) tCATGAGTAAGGTACTGGAAATGCTGACCACTTAATCgg < 1:3360830/39‑1 (MQ=255) aTGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGc > 1:2052786/1‑55 (MQ=255) aTGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGc > 1:1630450/1‑55 (MQ=255) aTGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGc > 1:1259020/1‑55 (MQ=255) aTGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTa > 1:1867411/1‑62 (MQ=255) aTGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTg < 1:3481030/71‑1 (MQ=255) aTGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTg < 1:950603/71‑1 (MQ=255) aTGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACT‑GTGTTATTATTGCCCGCTTATGTCGTCTg > 1:792262/1‑70 (MQ=255) aTGAGTAAGGTACTGGAAATGCTGACCACTTAATCGGGa > 1:3349788/1‑39 (MQ=255) aTGAGTAAGGTACTGGAAATGCTGACCACTTAATCGGGa > 1:2394615/1‑39 (MQ=255) gAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGccc > 1:2125042/1‑55 (MQ=255) gAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGccc > 1:2125078/1‑55 (MQ=255) gAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCa > 1:2377758/1‑71 (MQ=255) aGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATgtcgtc > 1:1355219/1‑66 (MQ=255) gTAAGGTACTGGATATGTTGACCACTTAATCGGGACt > 1:746565/1‑37 (MQ=255) gTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGttatta < 1:3426016/47‑1 (MQ=255) gTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGttatta < 1:2658374/47‑1 (MQ=255) gTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGcaca < 1:2497894/71‑1 (MQ=255) tACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACag < 1:2265629/71‑1 (MQ=255) cTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGc > 1:2264265/1‑65 (MQ=255) tGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:1477345/47‑1 (MQ=255) tGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCt < 1:2147266/47‑1 (MQ=255) ttGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTTCACAGAGGACCCTTc > 1:2517244/1‑70 (MQ=255) ttGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTc > 1:3393825/1‑70 (MQ=255) | GAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTC > W3110S.gb/2721219‑2721356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |