| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,737,004 | A→G | 15.0% | T202A (ACC→GCC) | pheA → | fused chorismate mutase P and prephenate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,737,004 | 0 | A | G | 15.0% | 89.7 / 9.0 | 40 | T202A (ACC→GCC) | pheA | fused chorismate mutase P and prephenate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (15/19); new base G (2/4); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
TCGATTGTTGGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGC > W3110S.gb/2736935‑2737073 | tCGATTGTTGGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATccac > 1:3185358/1‑71 (MQ=255) aTTGTTGGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCAt > 1:3817285/1‑71 (MQ=255) aTTGTTGGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCAt > 1:3259089/1‑71 (MQ=255) ttgttgGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATcca > 1:3850085/1‑66 (MQ=255) ttgttgGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATcca > 1:1755724/1‑66 (MQ=255) tgttgGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATc > 1:995845/1‑70 (MQ=255) ttgGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCaa < 1:2575714/70‑1 (MQ=255) ggCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATa < 1:1745685/70‑1 (MQ=255) ggCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATAc > 1:316142/1‑71 (MQ=255) gCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACg < 1:2121369/71‑1 (MQ=255) agaTGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACg < 1:3506410/68‑1 (MQ=255) tGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATAGGGTCTa < 1:496262/70‑1 (MQ=255) aCGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAg > 1:554599/1‑71 (MQ=255) cTATCGATCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCGCCATCAATACGGTCTACAGCCATCCg < 1:1461934/71‑1 (MQ=255) cTATCGATCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCGCCATCAATACGGTCTACAGCCATCCg < 1:2772689/71‑1 (MQ=255) tgtttgttGGTCTCCGGCACTACTGATTTAACCACCATCAATACGGTCTACAGCCATCCGCAGCCATTc > 1:1091509/1‑69 (MQ=255) tttgttGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGc < 1:3655242/71‑1 (MQ=255) tttgttGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGc < 1:237691/71‑1 (MQ=255) tttgttGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGc < 1:3337272/71‑1 (MQ=255) tgttgGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCaa > 1:1129586/1‑71 (MQ=255) tgttgGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCaa > 1:2489354/1‑71 (MQ=255) gttgGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCGATTc < 1:669060/64‑1 (MQ=255) ttgGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGACATTcc < 1:154/64‑1 (MQ=255) ggTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATg > 1:2239408/1‑69 (MQ=255) ggTCTCCGGCACTACTGATTTATCCACCATCAAAACGGTCTACAGCCATCCGCAGCCATTCCAGCAATg > 1:1984502/1‑69 (MQ=255) gTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATgca < 1:619514/70‑1 (MQ=255) tcCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATgcagca > 1:2038798/1‑70 (MQ=255) cGGCACTACTGATTTATCCGCCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCaaa < 1:1214744/70‑1 (MQ=255) cGGCACTACTGATTTATCCGCCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCaaa < 1:754917/70‑1 (MQ=255) cGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATgcagca < 1:3556853/68‑1 (MQ=255) cGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAAt > 1:3253124/1‑71 (MQ=255) actactGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCt < 1:311427/71‑1 (MQ=255) tactGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGc < 1:411611/44‑1 (MQ=255) actGATTTATCCGCCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGc > 1:3653526/1‑52 (MQ=255) actGATTTATCCGCCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGc > 1:2272482/1‑52 (MQ=255) actGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAAt > 1:3614110/1‑64 (MQ=255) ctGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTa < 1:2017596/69‑1 (MQ=255) ctGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTa < 1:783263/69‑1 (MQ=255) ctGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAAt > 1:2837325/1‑71 (MQ=255) aTTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGt < 1:3824836/71‑1 (MQ=255) aTTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGt < 1:2099481/71‑1 (MQ=255) tttATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGtt < 1:2838378/71‑1 (MQ=255) tATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTAt > 1:2168320/1‑71 (MQ=255) caccaTCAATACGGTCTACAGCCATCCGCAGCCATTcc < 1:606650/38‑1 (MQ=255) caccaTCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGc < 1:644507/71‑1 (MQ=255) | TCGATTGTTGGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGC > W3110S.gb/2736935‑2737073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |