| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,738,210 | G→A | 10.5% | V172V (GTC→GTT) | tyrA ← | fused chorismate mutase T and prephenate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,738,210 | 0 | G | A | 10.5% | 153.0 / 6.9 | 57 | V172V (GTC→GTT) | tyrA | fused chorismate mutase T and prephenate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (32/18); new base A (3/3); total (36/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACA > W3110S.gb/2738142‑2738279 | cccAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGAc > 1:897477/1‑71 (MQ=255) ccAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGAc > 1:3434410/1‑70 (MQ=255) aGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAg < 1:927560/71‑1 (MQ=255) cACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGaa > 1:852907/1‑71 (MQ=255) ggACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATAc > 1:515292/1‑70 (MQ=255) ggACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACa < 1:2045227/71‑1 (MQ=255) gACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACaa < 1:1981646/71‑1 (MQ=255) tcatGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCttt > 1:1879397/1‑71 (MQ=255) tcatGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCtt > 1:2004257/1‑70 (MQ=255) tGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCgg > 1:1592606/1‑71 (MQ=255) cgcCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTa < 1:508584/71‑1 (MQ=255) ccaccaGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTaaa < 1:354400/71‑1 (MQ=255) ccaccaGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTaaa < 1:897839/71‑1 (MQ=255) caGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCg < 1:993141/71‑1 (MQ=255) aGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTaaa > 1:3481027/1‑66 (MQ=255) gCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGt < 1:923688/71‑1 (MQ=255) aTGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTaa < 1:2814175/71‑1 (MQ=255) aTGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTaa > 1:265418/1‑71 (MQ=255) tGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCg < 1:2040759/38‑1 (MQ=255) gCCTGTAATGGTCCATTTTTCACCGATGCCAGATCAACCAGAATACAATCTTTCGGTAAAGGCGGTaa < 1:1344080/68‑1 (MQ=255) gCCTGTAATGGTCCATTTTTCACCGATGCCAGATCAACCAGAATACAATCTTTCGGTAAAGGCGGTaa < 1:418385/68‑1 (MQ=255) ccTGTAATGGTCCATTTTTCACCGATGCCAGATCAACCAGAATACAATCTTTCGGTAAAGGCGGTaa < 1:2123854/67‑1 (MQ=255) tAATGGCCCATTTTTCACTGATGCCAGATCGACCAGaa > 1:2252388/1‑38 (MQ=255) tAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTa < 1:276967/53‑1 (MQ=255) tAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCaa > 1:2295422/1‑71 (MQ=255) tAATGGCCCATTTTTAACTGATGCCAGATCGACCAGaa > 1:521677/1‑38 (MQ=255) aTGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATa < 1:1707769/71‑1 (MQ=255) aTTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCt > 1:1807054/1‑71 (MQ=255) tttttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGt < 1:2186047/51‑1 (MQ=255) tttttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCt > 1:647371/1‑70 (MQ=255) tttttCACCGATGCCAGATCAACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCGATAACTTGCt > 1:1897191/1‑70 (MQ=255) tttttCACCGATGCCAGATCAACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCGATAACTTGCt > 1:1698111/1‑70 (MQ=255) ttttCACCGATGCCAGATCAACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCGATAACTTGCt > 1:3671202/1‑69 (MQ=255) tttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAAt > 1:1028129/1‑52 (MQ=255) tttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAAt > 1:327439/1‑52 (MQ=255) tttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGc > 1:2319650/1‑56 (MQ=255) tttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATaa > 1:1119042/1‑62 (MQ=255) tttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAg > 1:539052/1‑71 (MQ=255) tttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAg > 1:775886/1‑71 (MQ=255) tttCACTGATGCCAGATCGACCAGAATACAATCTTTAGGTAAAGGCGGTAAtt > 1:1717312/1‑53 (MQ=255) ttCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCaa > 1:3842340/1‑58 (MQ=255) cTGATGCCAGATCGACCAGAATACAATCTTTCGGTa < 1:2714307/36‑1 (MQ=255) cTGATGCCAGATCGACCAGAATACAATCTTTCGGTa < 1:468168/36‑1 (MQ=255) tGATGCCAGATCGACCAGAATACAATCTTTCGGTaa > 1:3351226/1‑36 (MQ=255) tGATGCCAGATCGACCAGAATACAATCTTTCGGTaa > 1:2220064/1‑36 (MQ=255) aTGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAAt > 1:841864/1‑44 (MQ=255) aTGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTgg < 1:2563785/71‑1 (MQ=255) aTGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTg < 1:2639930/70‑1 (MQ=255) tGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTg < 1:2544157/69‑1 (MQ=255) tGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGa > 1:2667351/1‑71 (MQ=255) ccAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGAtt > 1:241234/1‑71 (MQ=255) gATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCt < 1:2642858/54‑1 (MQ=255) aTCTACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGc > 1:251242/1‑70 (MQ=255) aTCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGca > 1:2885045/1‑71 (MQ=255) aTCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGca > 1:1052114/1‑71 (MQ=255) tCGACCAGAATACAATCTTTCGGTAAAGGCGGTAAt > 1:805555/1‑36 (MQ=255) cGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGcaca > 1:2722932/1‑71 (MQ=255) cGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGcaca > 1:2084596/1‑71 (MQ=255) | CCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACA > W3110S.gb/2738142‑2738279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |