| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,852,710 | G→T | 10.8% | L253L (CTG→CTT) | hypE → | carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,852,710 | 0 | G | T | 10.8% | 138.4 / 2.9 | 46 | L253L (CTG→CTT) | hypE | carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein |
| Reads supporting (aligned to +/- strand): ref base G (17/24); new base T (2/3); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TCAGAAGCGGCACTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGT > W3110S.gb/2852642‑2852780 | tCAGAAGCGGCACTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTg < 1:3728468/69‑1 (MQ=255)tCAGAAGCGGCACTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTTaa > 1:3769935/1‑71 (MQ=255)tCAGAAGCGGCACTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTTaa > 1:3765569/1‑71 (MQ=255) cGGCACTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGcc < 1:484157/71‑1 (MQ=255) aCTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACg < 1:915864/71‑1 (MQ=255) tGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACg > 1:2193706/1‑69 (MQ=255) gCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAg > 1:1906725/1‑71 (MQ=255) ccTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAgg > 1:1067484/1‑71 (MQ=255) ccTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAgg > 1:157045/1‑71 (MQ=255) ttAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAg > 1:3402553/1‑66 (MQ=255) tAAACCTGCTGTGCGCGGCGTTTGCGAACTGCTGGGACTGGACGCCCTTAACt < 1:859657/53‑1 (MQ=255) tAAACCTGCTGTGCGCGGCGTTTGCGAACTGCTGGGACTGGACGCCCTTAACt < 1:280462/53‑1 (MQ=255) tAAACCTGCTGTGCGCGGCGTTTGCGAACTGCTGGGACTGGACGCCCTTAACt < 1:2740619/53‑1 (MQ=255) aaaCCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAAc > 1:957790/1‑70 (MQ=255) aaaCCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAAc > 1:561788/1‑70 (MQ=255) aCCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAg < 1:1478713/71‑1 (MQ=255) aCCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAg < 1:2430773/71‑1 (MQ=255) gtgcgtgGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAAt > 1:7294/1‑68 (MQ=255) gtgcgtgGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGAAAACTAGTAAt > 1:2344932/1‑68 (MQ=255) tgcgtgGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCt < 1:1768492/71‑1 (MQ=255) tgcgtgGCGTTAGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCt > 1:3143763/1‑71 (MQ=255) gCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACg < 1:3715208/45‑1 (MQ=255) gCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACg < 1:1397351/45‑1 (MQ=255) gCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACg < 1:3524827/45‑1 (MQ=255) tGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTaa > 1:1411218/1‑55 (MQ=255) tGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGaacgcaa > 1:1480777/1‑71 (MQ=255) gCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGaacgcaac > 1:1658895/1‑71 (MQ=255) gAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGaacgcaac < 1:312959/69‑1 (MQ=255) gAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGaacgcaac < 1:1989139/69‑1 (MQ=255) ttGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGaacgcaacg > 1:1891501/1‑67 (MQ=255) ttGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCgg < 1:3805166/70‑1 (MQ=255) ttGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCgg < 1:2498728/70‑1 (MQ=255) tGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCa < 1:1987316/39‑1 (MQ=255) gCTGGGACTGGACGCCCTGAACTTTGCCAACGAAg > 1:576322/1‑35 (MQ=255) ggACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGc < 1:817027/70‑1 (MQ=255) ggACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCa < 1:1969771/71‑1 (MQ=255) ggACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCa < 1:1974061/71‑1 (MQ=255) gACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCaa < 1:386700/71‑1 (MQ=255) tGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGc < 1:2120769/66‑1 (MQ=255) gACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTaa > 1:3830764/1‑37 (MQ=255) gACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCt < 1:2355747/71‑1 (MQ=255) gACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCt > 1:2638422/1‑71 (MQ=255) gACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCt < 1:422915/71‑1 (MQ=255) gCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTgg < 1:722731/70‑1 (MQ=255) cTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGt < 1:2724676/62‑1 (MQ=255) cTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGt < 1:3565199/62‑1 (MQ=255) tGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCa < 1:2218133/58‑1 (MQ=255) gAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGGACGCAACGCGGCAGAGCAAGTGCTGGCAGCGt > 1:3492246/1‑71 (MQ=255) gAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAg > 1:3271748/1‑59 (MQ=255) | TCAGAAGCGGCACTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGT > W3110S.gb/2852642‑2852780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |