| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,909,021 | G→A | 13.8% | N107N (AAC→AAT) | mazG ← | nucleoside triphosphate pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,909,021 | 0 | G | A | 13.8% | 145.0 / 12.6 | 58 | N107N (AAC→AAT) | mazG | nucleoside triphosphate pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (29/21); new base A (5/3); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
CGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGC > W3110S.gb/2908952‑2909088 | cGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGt > 1:222586/1‑71 (MQ=255) ctgcGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCgg < 1:667319/71‑1 (MQ=255) cgcTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAg < 1:819268/71‑1 (MQ=255) tCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGTCAAGCACTTCACTACTGTTTTCGGCAGAACt < 1:1601750/70‑1 (MQ=255) gcgcTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCaa < 1:1961861/71‑1 (MQ=255) ctctTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCaaaa < 1:1637604/70‑1 (MQ=255) ttCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTc < 1:1595570/48‑1 (MQ=255) ttCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACt < 1:2054089/57‑1 (MQ=255) ttCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCaaaa < 1:3831333/67‑1 (MQ=255) ttCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACAt > 1:1981790/1‑71 (MQ=255) cGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGaa > 1:516816/1‑53 (MQ=255) cGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGaa > 1:2861893/1‑53 (MQ=255) cGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGc > 1:3754057/1‑71 (MQ=255) cGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGc > 1:2471929/1‑71 (MQ=255) ttttGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCaa < 1:1124226/60‑1 (MQ=255) ttGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATg > 1:42847/1‑71 (MQ=255) tGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCa > 1:3676412/1‑44 (MQ=255) tGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATgg > 1:2501363/1‑71 (MQ=255) gATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGc > 1:1692569/1‑71 (MQ=255) aTTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGc < 1:2297952/70‑1 (MQ=255) tGCTCCCAAGGGGCAAGCACTTCACTACTATTTTCGGCAGa < 1:588922/41‑1 (MQ=38) tGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGa > 1:3410939/1‑69 (MQ=255) tGCTCCCAACGGGCAAGCACTTCACTACTATTTTCGGCAGa < 1:3002169/41‑1 (MQ=255) tGCTCCCAACGGGCAAGCACTTCACTACTATTTTCGGCAGa < 1:2871104/41‑1 (MQ=255) tGCTCCCAACGGGCAAGCACTTCACTACTATTTTCGGCAGAACTGTCAg > 1:601109/1‑49 (MQ=255) tGCTCCCAACGGGCAAGCACTTCACTACTATTTTCGGCAGAACTGTCAg > 1:1567707/1‑49 (MQ=255) tGCTCCCAACGGGCAAGCACTTCACTACTATTTTCGGCAGAACTGTCAg > 1:2242030/1‑49 (MQ=255) tGCTCCCAACGGGCAAGCACTTCACTACTATTTTCGGCAGAACTGTCAg > 1:1268963/1‑49 (MQ=255) tGCTCCCAACGGGCAAGCACTTCACTACTATTTTCGGCAGAACTGTCAg > 1:755666/1‑49 (MQ=255) gCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACg < 1:2463288/70‑1 (MQ=255) cTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGct > 1:3319076/1‑71 (MQ=255) cTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGc > 1:1957864/1‑70 (MQ=255) ccAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGctct > 1:770316/1‑70 (MQ=255) aaCGGGCAAGCACTTCACTACTGTTTTCGGCAGAACt < 1:2280270/37‑1 (MQ=255) aaCGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCaaaaa < 1:2320820/48‑1 (MQ=255) aCGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTAt > 1:2423809/1‑38 (MQ=255) aCGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTAt > 1:382177/1‑38 (MQ=255) cGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTAt > 1:3244492/1‑37 (MQ=255) cGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAAttt < 1:31270/71‑1 (MQ=255) gggCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCaaaaa < 1:1210401/45‑1 (MQ=255) gggCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGa > 1:2809483/1‑53 (MQ=255) gCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATc > 1:1080475/1‑71 (MQ=255) aGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGa > 1:3764826/1‑48 (MQ=255) gCACTTCACTACTGTTTTCGGCAGAACTATCAGCaaaaa < 1:1898271/39‑1 (MQ=255) gCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt > 1:2034606/1‑70 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAACCATGCGGATGGCGACGCTCTAATTTATCGCTaa < 1:1880184/71‑1 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTaa < 1:1131853/71‑1 (MQ=255) aCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTaa > 1:2099241/1‑70 (MQ=255) aCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAAt < 1:204144/71‑1 (MQ=255) ttCACTACTGTTTTCGGCAGAACTATCAGCaaaaa < 1:1508947/35‑1 (MQ=255) tCACTACTGTTTTCGGCAGAACTATCAGCAAAAACa > 1:3558978/1‑36 (MQ=255) tCACTACTGTTTTCGGCAGAACTATCAGCAAAAACa > 1:153789/1‑36 (MQ=255) tCACTACTGTTTTCGGCAGAACTATCAGCAAAAACa > 1:2011163/1‑36 (MQ=255) tCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATag > 1:1557915/1‑70 (MQ=255) cACTACTGTTTTCGGCAGAACTATCAGCAAAAAct > 1:1936061/1‑34 (MQ=255) ctGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAAtt > 1:1194072/1‑52 (MQ=255) ctGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAgcgc > 1:530816/1‑70 (MQ=255) tGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAAttt < 1:1800668/52‑1 (MQ=255) | CGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGC > W3110S.gb/2908952‑2909088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |