| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 181,321 | C→T | 10.4% | G146G (GGC→GGT) | degP → | serine endoprotease (protease Do), membrane‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 181,321 | 0 | C | T | 10.4% | 129.8 / 4.7 | 48 | G146G (GGC→GGT) | degP | serine endoprotease (protease Do), membrane‑associated |
| Reads supporting (aligned to +/- strand): ref base C (18/25); new base T (3/2); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCTATGTCGTCACCAACAACCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATCGCGCTGATCCAAATC > W3110S.gb/181254‑181384 | gCTATGTCGTCACCAACAACCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGt < 1:360033/71‑1 (MQ=255) cTATGTCGTCACCAACAACCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGt < 1:3342679/70‑1 (MQ=255) tATGTCGTCACCAACAACCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGt > 1:925897/1‑69 (MQ=255) tGTCGTCACCAACAACCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAg < 1:2857566/70‑1 (MQ=255) aCCAACAACCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCg < 1:1907888/67‑1 (MQ=255) acaacCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGAcgcg > 1:196394/1‑68 (MQ=255) aacCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGCCGCGAAGAt > 1:90166/1‑71 (MQ=255) aacCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGAt > 1:1472912/1‑71 (MQ=255) aacCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGAt > 1:2290993/1‑71 (MQ=255) acCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATg < 1:3471866/71‑1 (MQ=255) cACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGt < 1:317192/71‑1 (MQ=255) aCGTTGTTGATAACGCGACTGTCATTAAAGTTCAACTGAGCGATGGCCGTAAg < 1:1874611/53‑1 (MQ=255) gttgATAACGCGACGGTGATTAAAGTCCAACTGAGCGATGGTCGTAAGTTCGACGCGAAGATGGTTGGCa < 1:3791359/70‑1 (MQ=255) gttgATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCa > 1:324085/1‑70 (MQ=255) ttgATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTa < 1:2990654/45‑1 (MQ=255) ttgATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTa < 1:616415/45‑1 (MQ=255) ttgATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGaa < 1:3683389/58‑1 (MQ=255) ttgATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGaa < 1:2241351/58‑1 (MQ=255) ttgATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGt < 1:1769991/64‑1 (MQ=255) ttgATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGt < 1:480964/64‑1 (MQ=255) tAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAGGATGGTTGGCAAAGATc > 1:3183268/1‑71 (MQ=255) aaCGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTaa > 1:1651739/1‑41 (MQ=255) gcgACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGa > 1:3298536/1‑52 (MQ=255) gcgACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGa > 1:36847/1‑52 (MQ=255) gcgACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGa > 1:1784485/1‑52 (MQ=255) gcgACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCcgcg < 1:2201299/71‑1 (MQ=255) gcgACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCcgcg < 1:2945699/71‑1 (MQ=255) cgACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGtt > 1:1646363/1‑56 (MQ=255) cGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATc > 1:2834757/1‑63 (MQ=255) cGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATc > 1:1595330/1‑63 (MQ=255) gtgaTTAAAGTCCAACTGAGCGATGGTCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGa < 1:3010838/68‑1 (MQ=255) tCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGTTTGGCAAAg < 1:2493645/57‑1 (MQ=255) tCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAg < 1:2812566/57‑1 (MQ=255) tCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGat < 1:2195007/71‑1 (MQ=255) taTTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCaaa > 1:1315576/2‑55 (MQ=255) cATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCaaa > 1:1355468/1‑55 (MQ=255) aTTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGaa < 1:3454965/41‑1 (MQ=255) aTTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGaa < 1:1983799/41‑1 (MQ=255) aaaGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATcgc > 1:462021/1‑71 (MQ=255) tccAACTGAGCGATGGTCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATcgcg > 1:2623947/3‑68 (MQ=255) tccAACTGAGCGATGGTCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATcgcg > 1:2791227/3‑68 (MQ=255) tccAACTGAGCGATGGTCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATcgc > 1:3851740/3‑67 (MQ=255) cAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATCGCGCTGAt > 1:2178164/1‑71 (MQ=255) gCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATCGCGCTGATCCAAATc < 1:338034/71‑1 (MQ=255) gCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATCGCGCTGATCCAAATc < 1:2207718/71‑1 (MQ=255) gCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATCGCGCTGATCCAAATc < 1:3242074/71‑1 (MQ=255) tGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGa < 1:1023260/36‑1 (MQ=255) tGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGAt < 1:1056457/37‑1 (MQ=255) | GCTATGTCGTCACCAACAACCACGTTGTTGATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCTGATATCGCGCTGATCCAAATC > W3110S.gb/181254‑181384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |