| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,953,568 | G→A | 7.7% | S364S (AGC→AGT) | recB ← | exonuclease V (RecBCD complex), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,953,568 | 0 | G | A | 7.7% | 228.8 / 6.4 | 78 | S364S (AGC→AGT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (43/29); new base A (3/3); total (46/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
CTGAAATTCATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCACGGCGGCGTTTTT > W3110S.gb/2953499‑2953635 | cTGAAATTCATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGc > 1:1300208/1‑71 (MQ=255) aaaTTCATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTAc > 1:3115952/1‑71 (MQ=255) aTTCATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACg > 1:2629917/1‑70 (MQ=255) aTTCATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACg > 1:1560338/1‑70 (MQ=255) cATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAgc > 1:2753595/1‑71 (MQ=255) cATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAgc > 1:1212159/1‑71 (MQ=255) aTCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAATACCTCGCCGCTTTCACTACGCAgc < 1:2626844/70‑1 (MQ=255) gATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCCCCGCTTTCGCTACGCAGcgcg > 1:2083044/1‑70 (MQ=255) gATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGcgcg > 1:2423125/1‑70 (MQ=255) gATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGcgcg > 1:2159143/1‑70 (MQ=255) gATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCgg < 1:2736937/71‑1 (MQ=255) cATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAAt > 1:1556706/1‑71 (MQ=255) cATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAAt > 1:3701044/1‑71 (MQ=255) aTTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGa < 1:2993884/68‑1 (MQ=255) aTTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATc > 1:2177681/1‑71 (MQ=255) aTTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATc < 1:503982/71‑1 (MQ=255) tGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAAt > 1:822470/1‑68 (MQ=255) tGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATc > 1:407915/1‑69 (MQ=255) tGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATc > 1:2564844/1‑69 (MQ=255) tGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATc > 1:3419518/1‑69 (MQ=255) ccGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGa > 1:3324484/1‑71 (MQ=255) gggAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACt > 1:3239112/1‑71 (MQ=255) gggAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACt > 1:3243677/1‑71 (MQ=255) gggAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACt > 1:2133479/1‑71 (MQ=255) ggAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACtt < 1:2856445/71‑1 (MQ=255) gAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTa < 1:2597860/71‑1 (MQ=255) gAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTa < 1:789434/71‑1 (MQ=255) aTCGCGTACGGATCGCCGCTGCCAATACCTCGCCGCTTTCACTACGCAGCGCGGAATCGAg > 1:537272/1‑61 (MQ=255) aTCGCGTACGGATCGCCGCTGCCAATACCTCGCCGCTTTCACTACGCAGCGCGGAATCGAGCCGACTTaa > 1:1768972/1‑70 (MQ=255) aTCGCGTACGGATCGCCGCTGCCAATACCTCGCCGCTTTCACTACGCAGCGCGGAATCGAGCCGACTTa > 1:3149450/1‑69 (MQ=255) aTCGCGTACGGATCGCAGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTaa > 1:3550209/1‑70 (MQ=255) tCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCgg < 1:1972288/53‑1 (MQ=255) tCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCgg < 1:2720511/53‑1 (MQ=255) cgcgTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACAt > 1:2976430/1‑71 (MQ=255) gcgTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTa > 1:2278075/1‑41 (MQ=255) gcgTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTa > 1:2325876/1‑41 (MQ=255) gcgTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTa > 1:442646/1‑41 (MQ=255) tACGGATCGCCGCTGCCAATACCTCGCCGCTTTCACTACGCAGCGCGGAATCGAGCCGACTTAACATGtca < 1:3024148/71‑1 (MQ=255) tACGGATCGCCGCTGCCAATACCTCGCCGCTTTCACTACGCAGCGCGGAATCGAGCCGACTTAACATGtca < 1:903590/71‑1 (MQ=255) cGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGtcat > 1:3506040/1‑70 (MQ=255) cGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGtcat > 1:3499900/1‑70 (MQ=255) gATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCaa < 1:3017828/71‑1 (MQ=255) gATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCaa < 1:3315415/71‑1 (MQ=255) tCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCaaaa > 1:1645351/1‑71 (MQ=255) ccgcTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAAccc < 1:3739362/71‑1 (MQ=255) ccgcTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAAccc < 1:187824/71‑1 (MQ=255) ccgcTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAAccc < 1:76332/71‑1 (MQ=255) gcTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCaa < 1:584845/71‑1 (MQ=255) cTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAAt < 1:2712990/71‑1 (MQ=255) cTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAAt < 1:797647/71‑1 (MQ=255) gCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAAt > 1:3266603/1‑69 (MQ=255) ccAACAGCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCaa > 1:497688/1‑67 (MQ=255) ccAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTc < 1:3260674/70‑1 (MQ=255) ccAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCg < 1:940513/71‑1 (MQ=255) cAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAAc > 1:1545422/1‑62 (MQ=255) cAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGc < 1:1179994/71‑1 (MQ=255) cAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGc < 1:2643265/71‑1 (MQ=255) cAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGc < 1:707511/71‑1 (MQ=255) cAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGc < 1:412326/71‑1 (MQ=255) acCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGAc > 1:3288361/1‑40 (MQ=255) ccTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCAcgg > 1:1439902/1‑71 (MQ=255) cTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGc < 1:3185423/65‑1 (MQ=255) cTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGc < 1:1792203/65‑1 (MQ=255) tCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGtc < 1:3831407/47‑1 (MQ=255) tCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGtc < 1:556394/47‑1 (MQ=255) tCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAAccc < 1:1907152/57‑1 (MQ=255) aCCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACa > 1:204873/1‑41 (MQ=255) aCCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGtcat > 1:103516/1‑47 (MQ=255) aCCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGtcat > 1:2017791/1‑47 (MQ=255) aCCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGtcat > 1:3268266/1‑47 (MQ=255) cGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTaa > 1:1615184/1‑37 (MQ=255) cGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCTcggcggg > 1:2280115/1‑68 (MQ=255) cTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCaaaa > 1:1655224/1‑48 (MQ=255) cTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCaaaa > 1:3540307/1‑48 (MQ=255) cTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCACGGCGGCGttt < 1:2153598/71‑1 (MQ=255) cTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCACGGCGGCGt > 1:2749503/1‑69 (MQ=255) tCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCACGGCGGCGttttt < 1:1821598/70‑1 (MQ=255) tCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCACGGCGGCGttt > 1:1570550/1‑68 (MQ=255) | CTGAAATTCATCGATCATTGCCACCGGGAATCGCGTACGGATCGCCGCTGCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCCAATTCGCCACGGCGGCGTTTTT > W3110S.gb/2953499‑2953635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |