| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,982,115 | C→T | 10.1% | Q222Q (CAG→CAA) | kduI ← | predicted 5‑keto 4‑deoxyuronate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,982,115 | 0 | C | T | 10.1% | 162.6 / 7.2 | 59 | Q222Q (CAG→CAA) | kduI | predicted 5‑keto 4‑deoxyuronate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (22/31); new base T (2/4); total (24/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGG > W3110S.gb/2982049‑2982181 | caTCGACCAGCTCGGGGAGATCACCGCCTGCTCCTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcc > 1:1922557/2‑71 (MQ=255) cGACCAGCTCGGGGAGATCACCGCCTTCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCca > 1:700590/1‑71 (MQ=255) gACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCcat < 1:2135335/71‑1 (MQ=255) gACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCcat < 1:1467355/71‑1 (MQ=255) aCCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATGTGACGCGTTTCTTGCGGTTGCCCcatc < 1:3126847/71‑1 (MQ=255) aCCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATGTGACGCGTTTCTTGCGGTTGCCCcatc < 1:3435807/71‑1 (MQ=255) aCCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATGTGACGCGTTTCTTGCGGTTGCCCcatc < 1:754096/71‑1 (MQ=255) aCCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATGTGACGCGTTTCTTGCGGTTGCCCcatc < 1:1250621/71‑1 (MQ=255) cAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCcatcat < 1:1917973/71‑1 (MQ=255) aGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCAtg < 1:247520/71‑1 (MQ=255) ggggAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCca < 1:1758451/61‑1 (MQ=255) ggggAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCca < 1:2554145/61‑1 (MQ=255) ggggAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCca < 1:1869622/61‑1 (MQ=255) ggAGATCACCGCCTGCTCGTTATGCATCACAATGTGACGCGTTTCTTGCGGTTGCCCCATCAtg > 1:267016/1‑64 (MQ=255) ggAGATCACCGCCTGCTCGTTATGCATCACAATGTGACGCGTTTCTTGCGGTTGCCCCATCAtg > 1:2670373/1‑64 (MQ=255) agaTCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg < 1:923364/71‑1 (MQ=255) agaTCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg < 1:511130/71‑1 (MQ=255) agaTCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg < 1:3627914/71‑1 (MQ=255) cACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGc > 1:1340508/1‑46 (MQ=255) cACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGaaaa > 1:1438109/1‑65 (MQ=255) cACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATAATGTGGaaaa > 1:2660888/1‑65 (MQ=255) aCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCAtgtga < 1:977138/60‑2 (MQ=255) aCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGa < 1:1813915/61‑1 (MQ=255) ccGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCg > 1:3472425/1‑71 (MQ=255) ccGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCg < 1:97642/71‑1 (MQ=255) gCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGt > 1:3825520/1‑70 (MQ=255) cTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGtcat < 1:2163268/71‑1 (MQ=255) gTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCatcatc < 1:444529/69‑1 (MQ=255) gTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCatcatc < 1:143508/69‑1 (MQ=255) ttATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCAtg < 1:790316/45‑1 (MQ=255) ttATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg < 1:2377613/54‑1 (MQ=255) aTGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg > 1:1790018/1‑52 (MQ=255) aTGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGt > 1:1318880/1‑59 (MQ=255) gCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg > 1:1844444/1‑54 (MQ=255) gCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg > 1:1894237/1‑54 (MQ=255) cATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTga < 1:2673878/71‑1 (MQ=255) tCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTg < 1:3235839/68‑1 (MQ=255) cacaATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa > 1:179857/1‑70 (MQ=255) acaATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGaaaa > 1:1911417/1‑43 (MQ=255) acaATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGc > 1:1970514/1‑46 (MQ=255) acaATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGc > 1:3708728/1‑46 (MQ=255) acaATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa > 1:1215307/1‑69 (MQ=255) acaATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa > 1:1841632/1‑69 (MQ=255) caATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGaaaa > 1:1630301/1‑42 (MQ=255) caATATGACGCGTTTCTTGCGGCTGCCCAATCATGTGGaaaa > 1:2683962/1‑42 (MQ=255) aaTATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaatag > 1:1999982/1‑70 (MQ=255) aaTATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa < 1:644686/67‑1 (MQ=255) aaTATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa < 1:1513197/67‑1 (MQ=255) aCGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAACaa < 1:1652502/70‑1 (MQ=255) gcgTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTga < 1:2203942/57‑1 (MQ=255) tttCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaa < 1:3449934/55‑1 (MQ=255) tttCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaa < 1:267610/55‑1 (MQ=255) tttCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTcc < 1:3392173/71‑1 (MQ=255) tCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCAt > 1:187068/1‑71 (MQ=255) tCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCAt > 1:3338887/1‑71 (MQ=255) cTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATaa > 1:254352/1‑62 (MQ=255) gCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATc < 1:1033328/68‑1 (MQ=255) cGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCgg < 1:1114614/70‑1 (MQ=255) gCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATaa < 1:717318/56‑1 (MQ=255) gCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATaa < 1:2147326/56‑1 (MQ=255) | GATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGG > W3110S.gb/2982049‑2982181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |