| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,013,011 | C→T | 7.0% | D235N (GAC→AAC) | yqeC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,013,011 | 0 | C | T | 7.0% | 170.4 / 2.2 | 57 | D235N (GAC→AAC) | yqeC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (30/23); new base T (2/2); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACGGTT > W3110S.gb/3012943‑3013077 | cTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcgt < 1:1001695/71‑1 (MQ=255)cTAATTCACAAATCTGCGCGCGATTGCAGGATCTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcgt < 1:3412563/71‑1 (MQ=255) aTTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgtt > 1:157094/1‑71 (MQ=255) tCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgttgt > 1:1962116/1‑71 (MQ=255) cacaAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgttgt > 1:173014/1‑70 (MQ=255) cacaAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTgttgtt > 1:622218/1‑71 (MQ=255) cacaAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTgttgtt > 1:626662/1‑71 (MQ=255) acaAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTgttgtt > 1:933866/1‑70 (MQ=255) aaTCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAg > 1:3308629/1‑71 (MQ=255) cgcgcgATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTg < 1:2515794/71‑1 (MQ=255) cgcgcgATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTg < 1:3303490/71‑1 (MQ=255) aTTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGa < 1:3160276/66‑1 (MQ=255) cAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAg > 1:3481894/1‑67 (MQ=255) cAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg > 1:1083695/1‑71 (MQ=255) ggATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCt < 1:2683370/71‑1 (MQ=255) gATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTTGTGTTGTTGCAGCGGTTGAAg < 1:2035279/61‑1 (MQ=255) gATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTTGTGTTGTTGCAGCGGTTGAAg < 1:2890127/61‑1 (MQ=255) gATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGc < 1:2015724/65‑1 (MQ=255) gATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGc < 1:398349/65‑1 (MQ=255) aTGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTTGTGTTGTTGCAGCGGTTGAAGGAGCTCGCttt > 1:1773953/1‑71 (MQ=255) tGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa < 1:967751/49‑1 (MQ=255) gTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgtt < 1:1968025/42‑1 (MQ=255) gTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgtt < 1:1542127/42‑1 (MQ=255) cTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAgt > 1:1181360/1‑55 (MQ=255) cTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGc < 1:2430491/70‑1 (MQ=255) ttGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCaa > 1:2905827/1‑71 (MQ=255) gTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATc > 1:3282229/1‑71 (MQ=255) tataTCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCgg > 1:2336320/1‑45 (MQ=255) tatCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCa < 1:2417409/71‑1 (MQ=255) tatCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCa < 1:3216547/71‑1 (MQ=255) tCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCaa > 1:675890/1‑64 (MQ=255) cGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtc > 1:1193257/1‑71 (MQ=255) cGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtc > 1:3822239/1‑71 (MQ=255) gCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtct > 1:3578436/1‑71 (MQ=255) gCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtct > 1:986039/1‑71 (MQ=255) gCCAAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtct > 1:1452860/1‑71 (MQ=255) gccagATTGCCTCTACGTCGTGTTGTTGCAGCGGTTg < 1:2247536/37‑1 (MQ=255) gccagATTGCCTCTACGTCGTGTTGTTGCAGCGGTTg < 1:917438/37‑1 (MQ=255) cagATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACAtt > 1:2121656/1‑70 (MQ=255) agATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAg > 1:3830912/1‑37 (MQ=255) gATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAg < 1:2691921/36‑1 (MQ=255) gATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAg < 1:210890/36‑1 (MQ=255) gATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAg < 1:3750959/36‑1 (MQ=255) actGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCttt < 1:554698/44‑1 (MQ=255) tGCCTCTACGTTGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGCGCAATCGCATTCTCACATTGAGaa > 1:1034552/1‑70 (MQ=255) tGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGaa > 1:3842105/1‑70 (MQ=255) tGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGaa > 1:2604041/1‑70 (MQ=255) tGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGaa > 1:2326337/1‑70 (MQ=255) ctACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACgg > 1:3683563/1‑71 (MQ=255) ctACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACgg > 1:679132/1‑71 (MQ=255) ctACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACgg > 1:1459799/1‑71 (MQ=255) tACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCt < 1:1969379/36‑1 (MQ=255) tACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCt < 1:1666703/36‑1 (MQ=255) tACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCt < 1:1511530/36‑1 (MQ=255) tACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGc < 1:3757625/42‑1 (MQ=255) tACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGc < 1:122859/42‑1 (MQ=255) tACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATc > 1:126756/1‑46 (MQ=255) tACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATtct > 1:3764852/1‑53 (MQ=255) aCGTCGTGTTGTTGCAGCGGTTGAAGGAGCTAGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACGGt > 1:1005392/1‑70 (MQ=255) cgtcgtGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACGGtt < 1:2264735/70‑1 (MQ=255) gtcgtGTTGTTTCAGCGGTTGAAGGAGCTCGCTTTGTGc < 1:3653276/39‑1 (MQ=255) | CTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAACGGTT > W3110S.gb/3012943‑3013077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |