| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,035,613 | G→A | 9.1% | P384S (CCG→TCG) | recJ ← | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,035,613 | 0 | G | A | 9.1% | 122.4 / 2.7 | 44 | P384S (CCG→TCG) | recJ | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base G (19/21); new base A (2/2); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
CCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATA > W3110S.gb/3035548‑3035682 | ccccTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGt < 1:2381457/71‑1 (MQ=255) cTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaa < 1:1136361/71‑1 (MQ=255) cTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaa < 1:867043/71‑1 (MQ=255) cTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaa < 1:2220617/71‑1 (MQ=255) gAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaaa > 1:3078817/1‑71 (MQ=255) gAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaaa > 1:3667987/1‑71 (MQ=255) gAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaaa > 1:175440/1‑71 (MQ=255) cGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCttt < 1:2115375/71‑1 (MQ=255) cGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCttt < 1:410297/71‑1 (MQ=255) cGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCGCttt < 1:1474792/71‑1 (MQ=255) gACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTg < 1:258184/71‑1 (MQ=255) aCCGGAACCTTACAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGa < 1:2302241/71‑1 (MQ=255) ccGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGAt < 1:3242865/71‑1 (MQ=255) aaCCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATgcgc > 1:2648819/1‑71 (MQ=255) tttCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAg > 1:3160700/1‑71 (MQ=255) tCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGc > 1:3799758/1‑70 (MQ=255) aGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg < 1:64385/71‑1 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGctc < 1:2588222/47‑1 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATAcc < 1:2997074/71‑1 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATAc > 1:780643/1‑70 (MQ=255) cGTCACCTGCTGGCGCAAACGCGATAACCGAACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg < 1:939814/64‑1 (MQ=255) cGTCACCTGCTGGCGCAAACGCGATAACCGAACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg < 1:2304177/64‑1 (MQ=255) gTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCttt < 1:3160654/48‑1 (MQ=255) tCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGAc < 1:935521/71‑1 (MQ=255) tCACCTGCTGGCGCAAACGCGATAACCGAACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGaa > 1:3856372/1‑64 (MQ=255) tCACCTGCTGGCGCAAACGCGATAACCGAACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGaa > 1:3852201/1‑64 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACaa > 1:463/1‑71 (MQ=255) ctgctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCttt < 1:3699711/43‑1 (MQ=255) ctgctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCttt < 1:486394/43‑1 (MQ=255) ctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGccc > 1:374937/1‑71 (MQ=255) ctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGccc > 1:3192384/1‑71 (MQ=255) gcgcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:3307577/69‑1 (MQ=255) gcgcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:2591772/69‑1 (MQ=255) gcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATg > 1:2304006/1‑71 (MQ=255) aaCGCGATAACCGGACGGTGAAAACGCTCTTTGATgcgc > 1:2407613/1‑39 (MQ=255) aaCGCGATAACCGGACGGTGAAAACGCTCTTTGATgcgc > 1:3757905/1‑39 (MQ=255) aCGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGc < 1:366316/43‑1 (MQ=255) aCGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCa > 1:2085952/1‑70 (MQ=255) cgcgATAAGCGGACGGTGAAAACGCTCTTTGATgc > 1:2509786/1‑35 (MQ=255) cgcgATAACCGGACGGTGAAAACGCTCTTTGATgc > 1:1161457/1‑35 (MQ=255) cgcgATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAtt > 1:3861490/1‑71 (MQ=255) tAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACg > 1:3782788/1‑53 (MQ=255) tAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGcc > 1:3533129/1‑55 (MQ=255) ccGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGa < 1:472317/38‑1 (MQ=255) ggACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGaa > 1:1486090/1‑37 (MQ=255) ggACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATa < 1:3054691/71‑1 (MQ=255) ggACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATa < 1:1483746/71‑1 (MQ=255) ggACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATa < 1:3082141/71‑1 (MQ=255) gACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATa < 1:3757565/70‑1 (MQ=255) | CCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATA > W3110S.gb/3035548‑3035682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |