| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,085,574 | C→T | 12.7% | N71N (AAC→AAT) | metK → | methionine adenosyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,085,574 | 0 | C | T | 12.7% | 139.6 / 10.2 | 55 | N71N (AAC→AAT) | metK | methionine adenosyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base C (16/32); new base T (2/5); total (18/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
TGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTG > W3110S.gb/3085510‑3085640 | tGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGtt > 1:107103/1‑71 (MQ=255) gTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTc < 1:363274/70‑1 (MQ=255) ttAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTcgcg > 1:71154/1‑70 (MQ=255) aGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGaa < 1:2634821/70‑1 (MQ=255) ttGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAAt < 1:1044153/70‑1 (MQ=255) gcggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGc < 1:3319858/71‑1 (MQ=255) ggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTa < 1:1492418/71‑1 (MQ=255) ggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTa < 1:3557444/71‑1 (MQ=255) gcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTAt < 1:1031229/71‑1 (MQ=255) gcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTAt < 1:2545851/71‑1 (MQ=255) aaaTCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTAtgtg < 1:543389/71‑1 (MQ=255) aTCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAATACCGTTCGCGAAATTGGCTATGTGCa > 1:1668301/1‑71 (MQ=255) aTCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAATACCGTTCGCGAAATTGGCTATGTGCa > 1:1767542/1‑71 (MQ=255) aTCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCa > 1:641507/1‑71 (MQ=255) aTCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCa > 1:2366869/1‑71 (MQ=255) accaccAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTc > 1:3680630/1‑71 (MQ=255) caccaGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTcc < 1:2783220/70‑1 (MQ=255) ccaGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGAc < 1:588386/71‑1 (MQ=255) cgcCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTgg < 1:559640/51‑1 (MQ=255) cgcCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATgg < 1:2864776/71‑1 (MQ=255) ggTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCttt < 1:2750445/70‑1 (MQ=255) ggTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCtt < 1:3038601/69‑1 (MQ=255) tAGACATCGAAGATATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGAc < 1:2583708/59‑1 (MQ=255) aGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGATTTGACg > 1:1145339/1‑71 (MQ=255) gACATCGAAGAGATCACCGGTAACACCGTTCGCGAAATTGGCTa < 1:2314056/44‑1 (MQ=255) gACATCGAAGAGATCACCCGTAACACCGTTCGCGaaa > 1:985366/1‑37 (MQ=255) gACATCGAAGAGATCACCCGTAACACCGTTCGCGaaa > 1:2377879/1‑37 (MQ=255) gACATCGAAGAGATCACCCGTAACACCGTTCGCGa < 1:1269237/35‑1 (MQ=255) gACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTa < 1:2221237/44‑1 (MQ=255) gACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTa < 1:3839778/44‑1 (MQ=255) cATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTa < 1:1254209/71‑1 (MQ=255) cATCGAAGAGATCACCCGTAACAACGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTa < 1:529701/71‑1 (MQ=255) tCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTAtgtg < 1:2212075/44‑1 (MQ=255) tCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTAtgtg < 1:3283588/44‑1 (MQ=255) cGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATgg > 1:2235251/1‑56 (MQ=255) gAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACt < 1:286033/70‑1 (MQ=255) gAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACt < 1:2959297/70‑1 (MQ=255) gAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTc < 1:1184937/71‑1 (MQ=255) gAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTc > 1:2494529/1‑71 (MQ=255) aaGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTcc < 1:3628799/71‑1 (MQ=255) aaGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTcc < 1:3150228/71‑1 (MQ=255) agagATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGCCGCTAACTCCt > 1:2111560/1‑71 (MQ=255) agagATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCt > 1:3101173/1‑71 (MQ=255) ccGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCttt < 1:3252773/49‑1 (MQ=255) ccGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc > 1:2708140/1‑71 (MQ=255) ccGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc > 1:1809825/1‑71 (MQ=255) cGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACt < 1:1318124/58‑1 (MQ=255) cGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCt > 1:2963983/1‑71 (MQ=255) ttAATACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc < 1:2923890/68‑1 (MQ=255) gTAATACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc < 1:810474/69‑1 (MQ=255) gTAATACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc < 1:3615424/69‑1 (MQ=255) gTAATACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc < 1:422832/69‑1 (MQ=255) gTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCt > 1:1675122/1‑70 (MQ=255) gTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTg < 1:624306/71‑1 (MQ=255) tAATACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc < 1:2024643/68‑1 (MQ=255) | TGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTG > W3110S.gb/3085510‑3085640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |