| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 181,474 | T→C | 10.8% | F197F (TTT→TTC) | degP → | serine endoprotease (protease Do), membrane‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 181,474 | 0 | T | C | 10.8% | 93.0 / 3.5 | 37 | F197F (TTT→TTC) | degP | serine endoprotease (protease Do), membrane‑associated |
| Reads supporting (aligned to +/- strand): ref base T (16/17); new base C (2/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TAAGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAAC > W3110S.gb/181411‑181543 | tAAGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACAGTAGCGATTGGTAACCCGTTTg > 1:1600647/1‑65 (MQ=255)tAAGATGGCGGATTCTGAGGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTgg > 1:1831906/1‑71 (MQ=255) aTGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCga < 1:378721/71‑1 (MQ=255) tGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCga < 1:191559/70‑1 (MQ=255) ggCGGATTCTGATGCACTGCGCGTGTGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCga < 1:47185/69‑1 (MQ=255) gATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt < 1:1092572/71‑1 (MQ=255) gATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt < 1:122811/71‑1 (MQ=255) cTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGAc > 1:2713829/1‑64 (MQ=255) cTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTc < 1:1323700/65‑1 (MQ=255) gcgcGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTg > 1:170139/1‑71 (MQ=255) gTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTggg > 1:3831391/1‑44 (MQ=255) gTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGtctc > 1:577142/1‑71 (MQ=255) ggTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTgc < 1:1059852/71‑1 (MQ=255) tGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTgcgc < 1:3752716/71‑1 (MQ=255) aTTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTcc < 1:3357908/53‑1 (MQ=255) tACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTggg > 1:1796357/1‑71 (MQ=255) ccGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGt < 1:889632/71‑1 (MQ=255) tAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTgc < 1:3173072/58‑1 (MQ=255) tAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTgc < 1:2719639/58‑1 (MQ=255) tAGCAATTGGTAACCCGTTCGGCCTGGGCGAGACAGTAACTTCCgg < 1:2417259/46‑1 (MQ=25) tAGCAATTGGTAACCCGTTCGGCCTGGGCGAGACAGTAACTTCCgg < 1:842966/46‑1 (MQ=25) cGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGc < 1:3656703/71‑1 (MQ=255) ttGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTgg > 1:3849395/1‑57 (MQ=255) ttGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTgg > 1:695244/1‑57 (MQ=255) ttGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTgg > 1:228397/1‑57 (MQ=255) ttGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTgg > 1:2288504/1‑57 (MQ=255) ttGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCt > 1:2184672/1‑70 (MQ=255) ttGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:2667050/71‑1 (MQ=255) gTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGaa < 1:2558634/70‑1 (MQ=255) tAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCgg < 1:1206905/36‑1 (MQ=255) aaCCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGc > 1:2711869/1‑71 (MQ=255) aaCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGTAGCGGCCTGAACGc > 1:2472989/1‑71 (MQ=255) aaCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGTAGCGGCCTGAACGc > 1:1337091/1‑71 (MQ=255) aCCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGc > 1:2315034/1‑54 (MQ=255) aCCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTACGCTGGGGc > 1:794684/1‑54 (MQ=255) cGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGaa < 1:2133750/71‑1 (MQ=255) cGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGa > 1:426476/1‑70 (MQ=255) tttGGTCTGGGCGAGACGGTAACTTCCGGGATTGTctct < 1:1692414/39‑1 (MQ=255) tttGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:197667/60‑1 (MQ=255) tttGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:1025532/60‑1 (MQ=255) tGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGc > 1:3545710/1‑63 (MQ=255) tGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAAc > 1:421294/1‑70 (MQ=255) | TAAGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAAC > W3110S.gb/181411‑181543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |