| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,115,678 | A→T | 8.3% | R697R (CGT→CGA) | yghJ ← | predicted inner membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,115,678 | 0 | A | T | 8.3% | 218.7 / 11.3 | 84 | R697R (CGT→CGA) | yghJ | predicted inner membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (35/42); new base T (2/5); total (37/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
TTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCA > W3110S.gb/3115612‑3115745 | tttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACg > 1:1056748/1‑69 (MQ=255)tttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACg > 1:3389919/1‑69 (MQ=255)tttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACg > 1:2876618/1‑69 (MQ=255)tttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACg > 1:1547495/1‑69 (MQ=255)tttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACg > 1:1459796/1‑69 (MQ=255)tttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACg > 1:3164159/1‑69 (MQ=255) ttCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCgg < 1:901259/71‑1 (MQ=255) ttCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCgg < 1:2421646/71‑1 (MQ=255) tAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTg < 1:3045163/70‑1 (MQ=255) tAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGc < 1:1697788/71‑1 (MQ=255) gCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATa < 1:2989864/71‑1 (MQ=255) aTCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAgg < 1:1959426/71‑1 (MQ=255) cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTacac > 1:1931337/1‑50 (MQ=255) cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCAc > 1:3196135/1‑50 (MQ=255) cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGa > 1:928767/1‑59 (MQ=255) cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCa > 1:2974352/1‑64 (MQ=255) cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGAt > 1:1287734/1‑71 (MQ=255) cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTCGTATCTGCACGCAGCGGGATTGCAt > 1:912968/1‑65 (MQ=255) aGATCGGTCACATCCTGCTTAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATc > 1:2220741/1‑71 (MQ=255) aGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGAt < 1:1998627/70‑1 (MQ=255) aTCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGt < 1:3591250/71‑1 (MQ=255) cGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTa > 1:1906553/1‑71 (MQ=255) cGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTa > 1:2465289/1‑71 (MQ=255) cGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTa > 1:2129219/1‑71 (MQ=255) ggTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTa < 1:1602838/70‑1 (MQ=255) ggTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTAc < 1:1874370/71‑1 (MQ=255) gTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTAc < 1:2799517/70‑1 (MQ=255) acaTCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCAc > 1:1297999/1‑71 (MQ=255) acaTCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCAc > 1:910355/1‑71 (MQ=255) ctgctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGa < 1:2145942/70‑1 (MQ=255) ctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTca < 1:1892937/71‑1 (MQ=255) tgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTca < 1:2014781/70‑1 (MQ=255) tgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTca < 1:1444824/70‑1 (MQ=255) aGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGt > 1:545824/1‑53 (MQ=255) aGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGt > 1:2678896/1‑53 (MQ=255) aGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTcac < 1:2898208/69‑1 (MQ=255) aGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTcac < 1:957597/69‑1 (MQ=255) aGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTcac < 1:2696601/69‑1 (MQ=255) tCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTcaca < 1:2799909/68‑1 (MQ=255) tCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTcaca < 1:1194582/68‑1 (MQ=255) agattCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTc < 1:2834054/68‑1 (MQ=255) aGCTTCGGTTTGCTGGTATCTGCTCGCAGCGGGATTGCATAAGGATCGt > 1:385380/1‑49 (MQ=255) aGCTTCGGTTTGCTGGTATCTGCTCGCAGCGGGATTGCATAAGGATCGt > 1:2410814/1‑49 (MQ=255) aGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATc > 1:2599402/1‑47 (MQ=255) aGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATc > 1:1866639/1‑47 (MQ=255) aGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGa < 1:3429764/60‑1 (MQ=255) gCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTTAGTCACATATTc < 1:3716286/70‑1 (MQ=255) gCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTc < 1:325465/70‑1 (MQ=255) gCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTc < 1:1944635/70‑1 (MQ=255) gCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTc < 1:3075239/70‑1 (MQ=255) ttCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTg < 1:3317700/56‑1 (MQ=255) ttCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCaa > 1:1003935/1‑70 (MQ=255) tCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAg > 1:1140424/1‑71 (MQ=255) tCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAg > 1:3198305/1‑71 (MQ=255) tCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAg > 1:2814184/1‑71 (MQ=255) cGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTAc > 1:2106468/1‑47 (MQ=255) gTTTGCTGGTATCTGCTCGCAGCGGGATTGCATAAGGAt < 1:1885676/39‑1 (MQ=255) gTTTGCTGGTATCTGCTCGCAGCGGGATTGCATAAGGAt < 1:3619194/39‑1 (MQ=255) gTTTGCTGGTATCTGCTCGCAGCGGGATTGCATAAGGAt < 1:1298514/39‑1 (MQ=255) gTTTGCTGGTATCTGCTCGCAGCGGGATTGCATAAGGAt < 1:3813349/39‑1 (MQ=255) gTTTGCTGGTATCTGCTCGCAGCGGGATTGCATAAGGAt < 1:3390837/39‑1 (MQ=255) gTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTca < 1:2019202/57‑1 (MQ=255) tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCAc > 1:2585701/1‑48 (MQ=255) tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCAc > 1:1319697/1‑48 (MQ=255) tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCAc > 1:2884695/1‑48 (MQ=255) tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGt < 1:537013/71‑1 (MQ=255) tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGt < 1:2077304/71‑1 (MQ=255) cttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGt < 1:3859442/70‑1 (MQ=255) gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTa < 1:946509/70‑1 (MQ=255) gCTGGTATCTGCACGCAGCGGGATTGCATAAGGACCGTTACCCACCTGAGTCACATATTCAAAGCCGTTa < 1:1607940/70‑1 (MQ=255) tGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTca < 1:2920947/51‑1 (MQ=255) gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAgg < 1:989590/69‑1 (MQ=255) gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGa < 1:874501/70‑1 (MQ=255) gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGa < 1:1155445/70‑1 (MQ=255) tATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCGAAGCCGTTAAGGAt > 1:2801051/1‑70 (MQ=255) aTCTGCACGCAGCGGGATTGCATAAGGCTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGa > 1:2438877/1‑68 (MQ=255) aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGAt > 1:2100905/1‑69 (MQ=255) tCTGCACGCAGCGGGATTGCATAAGGATCGTTAcc < 1:3254793/35‑1 (MQ=255) tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcag > 1:1887576/1‑71 (MQ=255) tGCACGCAGCGGGATTGCATAAGGATCGTTACCCAcc > 1:1177336/1‑37 (MQ=255) cACGCAGCGGGATTGCATAAGTATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGTATcag < 1:3357055/67‑1 (MQ=255) cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATAtt > 1:541780/1‑48 (MQ=255) cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagca < 1:3715046/69‑1 (MQ=255) cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcag < 1:837036/67‑1 (MQ=255) cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcag < 1:1022453/67‑1 (MQ=255) | TTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCA > W3110S.gb/3115612‑3115745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |