| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,237,592 | G→A | 13.2% | V119V (GTG→GTA) | alx → | predicted inner membrane protein, part of terminus |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,237,592 | 0 | G | A | 13.2% | 134.2 / 10.2 | 53 | V119V (GTG→GTA) | alx | predicted inner membrane protein, part of terminus |
| Reads supporting (aligned to +/- strand): ref base G (25/21); new base A (5/2); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 | |||||||||||
GCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACT > W3110S.gb/3237526‑3237659 | gCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGc < 1:823486/68‑1 (MQ=255)gCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGc < 1:3073739/68‑1 (MQ=255) cTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTACTCgg < 1:3565057/71‑1 (MQ=255) cTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTACTCgg < 1:1969327/71‑1 (MQ=255) aTGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGc > 1:2555665/1‑71 (MQ=255) ttCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGt < 1:1545532/71‑1 (MQ=255) ttCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGt < 1:1713978/71‑1 (MQ=255) ttCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGt < 1:324470/71‑1 (MQ=255) cTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGc > 1:1856950/1‑71 (MQ=255) ttCTCTGTTCCGGCGTCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTAc > 1:2064247/1‑71 (MQ=255) tctcTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTAc > 1:1670592/1‑70 (MQ=255) ctctGTTCCGGCGGCATT‑‑AACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACcat < 1:2217934/70‑1 (MQ=255) ttCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACcatca < 1:2322882/69‑1 (MQ=255) ttCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATg < 1:2509808/71‑1 (MQ=255) tCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTACTCGGGGCGATTGTTCTGCGTACCATCATGa > 1:3578715/1‑71 (MQ=255) tCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTACTCGGGGCGATTGTTCTGCGTACCATCATGa > 1:3009971/1‑71 (MQ=255) ccGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGa > 1:250997/1‑70 (MQ=255) ggcggcATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCt > 1:296598/1‑71 (MQ=255) ggcggcATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCt > 1:1300727/1‑71 (MQ=255) gcggcATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCtt < 1:946329/71‑1 (MQ=255) gcggcATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCtt < 1:2179781/71‑1 (MQ=255) gcggcATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCtt < 1:1848914/71‑1 (MQ=255) ggcATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCa < 1:3378457/71‑1 (MQ=255) cATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCa > 1:3128573/1‑69 (MQ=255) aTTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATg > 1:2007268/1‑61 (MQ=255) aTTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACt > 1:989954/1‑70 (MQ=255) ttACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATg < 1:1310419/60‑1 (MQ=255) ttACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATg < 1:2026379/60‑1 (MQ=255) tACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATg < 1:2025470/59‑1 (MQ=255) aCAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTgg > 1:980892/1‑69 (MQ=255) aCAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGAGTACCATCATGATCTTCACTgg > 1:2723883/1‑69 (MQ=255) aCGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCt > 1:2074119/1‑71 (MQ=255) aCGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCt > 1:2906220/1‑71 (MQ=255) aCGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCt > 1:1803602/1‑71 (MQ=255) cgccgcGTGCTGGTGTATGGCGTGCTCGGGGCGATTg < 1:1423870/37‑1 (MQ=255) cgcgTGCTGGTGTATGGCGTACTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGTAGCTGGCt > 1:3422109/1‑71 (MQ=255) cgcgTGCTGGTGTATGGCGTACTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGTAGCTGGCt > 1:3791049/1‑71 (MQ=255) cgTGCTGGTGTATGGCGTACTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGTAGCTGGCtga > 1:3756291/1‑69 (MQ=255) gTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAAt < 1:2920611/71‑1 (MQ=255) cTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTc > 1:3813256/1‑51 (MQ=255) tgtATGGCGTGCTCTGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAg < 1:1871110/71‑1 (MQ=255) tgtATGGCGTGCTCTGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAg < 1:1875456/71‑1 (MQ=255) tATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCtt < 1:3695136/44‑1 (MQ=255) tATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCtt < 1:2001072/44‑1 (MQ=255) tGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTc > 1:729968/1‑70 (MQ=255) tGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTc > 1:810997/1‑70 (MQ=255) tGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTc > 1:2726153/1‑70 (MQ=255) tGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTc > 1:1017423/1‑70 (MQ=255) tGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATCATCTTCACTGGCAGCt > 1:795218/1‑53 (MQ=255) gCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGc > 1:1016957/1‑50 (MQ=255) gCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGc > 1:2159043/1‑50 (MQ=255) gCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGc > 1:3835548/1‑50 (MQ=255) cGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACt > 1:1628010/1‑71 (MQ=255) | GCTGATGTTGTTCAGCTATTTCTCTGTTCCGGCGGCATTACAACGCCGCGTGCTGGTGTATGGCGTGCTCGGGGCGATTGTTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACT > W3110S.gb/3237526‑3237659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |