| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,264,126 | T→C | 6.0% | E249E (GAA→GAG) | tdcC ← | L‑threonine/L‑serine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,264,126 | 0 | T | C | 6.0% | 180.2 / 2.4 | 66 | E249E (GAA→GAG) | tdcC | L‑threonine/L‑serine transporter |
| Reads supporting (aligned to +/- strand): ref base T (34/28); new base C (2/2); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
TAAAGGCAAAGAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACGAAGGAAGAGACGA > W3110S.gb/3264056‑3264193 | tAAAGGCAAAGAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGt > 1:3375968/1‑71 (MQ=255) ggCAAAGAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCgg > 1:1944989/1‑71 (MQ=255) aaaGAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGtt < 1:2182738/65‑1 (MQ=255) aaaGAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTg < 1:3534989/70‑1 (MQ=255) gAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGaa > 1:3513909/1‑69 (MQ=255) gAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGt > 1:3660013/1‑71 (MQ=255) aCATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGt < 1:1935685/64‑1 (MQ=255) aTCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTcgc > 1:2193575/1‑70 (MQ=255) aTCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTcgc > 1:1445313/1‑70 (MQ=255) accacTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGAcc > 1:2270531/1‑71 (MQ=255) ccacTGCAACCATCAGCATACTGGCGCGAGAGATGATTTGTGAACATTTACGCTCGGTGAAGTcgc < 1:2210019/66‑1 (MQ=255) ccacTGCAACCATCAGCATACTGGCGCGAGAGATGATTTGTGAACATTTACGCTCGGTGAAGTcgc < 1:1606185/66‑1 (MQ=255) cacTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCg < 1:1667432/70‑1 (MQ=255) cATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtct > 1:2290643/1‑71 (MQ=255) cATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtct > 1:1538501/1‑71 (MQ=255) cATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtc > 1:2610484/1‑70 (MQ=255) aTCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCg > 1:3062810/1‑45 (MQ=255) aTCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTc < 1:1763979/53‑1 (MQ=255) aTCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTc < 1:2264553/53‑1 (MQ=255) aTCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCttt > 1:595188/1‑68 (MQ=255) aTCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCttt > 1:1235161/1‑68 (MQ=255) tCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCg < 1:2493506/59‑1 (MQ=255) tCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCg < 1:2788756/59‑1 (MQ=255) tCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGaa < 1:564992/61‑1 (MQ=255) tCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGaa < 1:1239618/61‑1 (MQ=255) tCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCa > 1:233424/1‑71 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGaa > 1:423299/1‑59 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGaa > 1:51770/1‑59 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGaa > 1:2981470/1‑59 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGaa > 1:3473434/1‑59 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGaa > 1:2837251/1‑59 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCttt > 1:2447578/1‑65 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtc > 1:2636595/1‑66 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATa > 1:2458965/1‑71 (MQ=255) aGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATa > 1:3830184/1‑71 (MQ=255) aTGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATActc < 1:845562/71‑1 (MQ=255) tGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGa < 1:3199737/54‑1 (MQ=255) tGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGa < 1:1647342/54‑1 (MQ=255) tGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCa < 1:1065530/65‑1 (MQ=255) cTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCtt < 1:622398/70‑1 (MQ=255) cTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCtt < 1:362823/70‑1 (MQ=255) cTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGAGACCGAAGTCTTTCTCATACTCtt > 1:3320500/1‑70 (MQ=255) agaAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCtt < 1:286074/50‑1 (MQ=255) agaAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCtt < 1:2551759/50‑1 (MQ=255) agaAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCt > 1:1455862/1‑68 (MQ=255) aTGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGaaa > 1:2998439/1‑70 (MQ=255) aTGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAAc > 1:1889884/1‑71 (MQ=255) aTGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAAc < 1:1488348/71‑1 (MQ=255) gATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCa > 1:199852/1‑71 (MQ=255) aTTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCa > 1:1399178/1‑57 (MQ=255) aTTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCa > 1:3674856/1‑57 (MQ=255) aTTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAAc > 1:675982/1‑68 (MQ=255) aTTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCAc > 1:3661501/1‑71 (MQ=255) tttGTGAACATTTACGCTCGGTGAAGTCGCGACCAAAATCTTTCTCGTACTCTTCACGTTTAGAAACCAc > 1:3420448/1‑70 (MQ=255) tttGTGAACATTTACGCTCGGTGAAGTCGCGACCAAAATCTTTCTCGTACTCTTCACGTTTAGAAACCAc > 1:143210/1‑70 (MQ=255) tttGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtct < 1:93247/45‑1 (MQ=255) tttGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtct < 1:3520563/45‑1 (MQ=255) tttGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtct < 1:1559797/45‑1 (MQ=255) tttGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTtct < 1:2529202/45‑1 (MQ=255) ttGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTa > 1:3272372/1‑61 (MQ=255) gAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACgaagga < 1:2227501/71‑1 (MQ=255) aaCATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACgaaggaa < 1:825237/71‑1 (MQ=255) aaCATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACgaagg > 1:1444964/1‑69 (MQ=255) tttACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGaa < 1:3306759/55‑1 (MQ=255) tACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACGAAGGAAGAGAc < 1:2675500/70‑1 (MQ=255) tACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACGAAGGAAGAGAc < 1:3458925/70‑1 (MQ=255) aCGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACGAAGGAAGAGACGa > 1:199838/1‑71 (MQ=255) gTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACGAAGGAAGAGACg < 1:2650414/68‑1 (MQ=255) | TAAAGGCAAAGAACATCACCACTGCAACCATCAGCATGCTGGCACGAGAAATGATTTGGGAACATTTACGTTCGGTGAAGTCGCGACCGAAGTCTTTCTCATACTCTTCACGCTTAGAAACCACGAAGGAAGAGACGA > W3110S.gb/3264056‑3264193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |