| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,285,692 | A→G | 17.6% | G120G (GGA→GGG) | agaD → | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,285,692 | 0 | A | G | 17.6% | 73.6 / 9.4 | 34 | G120G (GGA→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Reads supporting (aligned to +/- strand): ref base A (13/15); new base G (3/3); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
ACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTA > W3110S.gb/3285623‑3285754 | aCTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGaa < 1:1647203/71‑1 (MQ=255) gTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAAtt > 1:3364990/1‑70 (MQ=255) cAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCtcatca < 1:3723649/71‑1 (MQ=255) tCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAAt < 1:1513757/58‑1 (MQ=255) tCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAAt < 1:2274355/58‑1 (MQ=255) tCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTcatcat < 1:669878/69‑1 (MQ=255) tCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTcatcat < 1:2999605/69‑1 (MQ=255) tCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCAttt < 1:3671605/71‑1 (MQ=255) tCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCAtt > 1:3390849/1‑70 (MQ=255) cGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTg > 1:3828651/1‑71 (MQ=255) gCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTgc > 1:2591348/1‑71 (MQ=255) ggATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTcatcat < 1:3553489/63‑1 (MQ=255) gATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTgccagcc < 1:3009389/71‑1 (MQ=255) ggCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAgg > 1:257320/1‑70 (MQ=255) tgcgatTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCtcatc < 1:1988291/51‑1 (MQ=255) tgcgatTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCtcatc < 1:135075/51‑1 (MQ=255) tgcgatTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCATCCAGGGAAAcc < 1:2556018/71‑1 (MQ=255) cgatTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGatt > 1:2698072/1‑38 (MQ=255) tttCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCtc > 1:1059585/1‑42 (MQ=255) tttCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAAc > 1:2268544/1‑64 (MQ=255) ttCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTgg < 1:1930675/70‑1 (MQ=255) ttCTGGTTTACCTTGTTGCCAATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAGGGGAACctgttg > 1:2277954/1‑69 (MQ=255) cTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGaaa > 1:2818685/1‑60 (MQ=255) cTGGTTTACTTTGTTGACGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGaaa > 1:980420/1‑60 (MQ=255) tACCTTTTTGCCAATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAgggg < 1:3050803/52‑2 (MQ=19) tACCTTGTTGCCTATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAgggg < 1:435324/52‑2 (MQ=25) tACCTTGTTGCCAATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAgggg < 1:1839672/52‑2 (MQ=25) tttGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGccagccag > 1:1397923/1‑46 (MQ=255) tttGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTgg > 1:213939/1‑59 (MQ=255) tttGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTgg > 1:490224/1‑59 (MQ=255) tATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAAc > 1:2197917/1‑40 (MQ=255) tATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACct < 1:1144370/42‑1 (MQ=255) aTGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGttt < 1:102421/70‑1 (MQ=255) ggCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTa < 1:623632/69‑1 (MQ=255) gCGGGGATTTGCTCATCGTTTGCCAGTCAGGGGAACctgttg > 1:727635/1‑42 (MQ=19) gCGGGGATTTGCTCATCGTTTGCCAGTCAGGGGAACCTGTTgg > 1:3743939/1‑43 (MQ=19) | ACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTA > W3110S.gb/3285623‑3285754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |