| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,285,713 | C→T | 24.2% | S127S (AGC→AGT) | agaD → | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,285,713 | 0 | C | T | 24.2% | 42.2 / 8.9 | 25 | S127S (AGC→AGT) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (10/8); new base T (3/3); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGC > W3110S.gb/3285644‑3285774 | gATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTgccagcc < 1:3009389/71‑1 (MQ=255) ggCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAgg > 1:257320/1‑70 (MQ=255) tgcgatTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCATCCAGGGAAAcc < 1:2556018/71‑1 (MQ=255) tttCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAAc > 1:2268544/1‑64 (MQ=255) ttCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTgg < 1:1930675/70‑1 (MQ=255) ttCTGGTTTACCTTGTTGCCAATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAGGGGAACctgttg > 1:2277954/1‑69 (MQ=255) cTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGaaa > 1:2818685/1‑60 (MQ=255) cTGGTTTACTTTGTTGACGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGaaa > 1:980420/1‑60 (MQ=255) tACCTTTTTGCCAATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAgggg < 1:3050803/52‑2 (MQ=19) tACCTTGTTGCCTATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAgggg < 1:435324/52‑2 (MQ=25) tACCTTGTTGCCAATTATGGCGGGGATTTGCTCATCGTTTGCCAGTCAgggg < 1:1839672/52‑2 (MQ=25) tttGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGccagccag > 1:1397923/1‑46 (MQ=255) tttGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTgg > 1:490224/1‑59 (MQ=255) tttGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTgg > 1:213939/1‑59 (MQ=255) tATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAAc > 1:2197917/1‑40 (MQ=255) tATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACct < 1:1144370/42‑1 (MQ=255) aTGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGttt < 1:102421/70‑1 (MQ=255) ggCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTa < 1:623632/69‑1 (MQ=255) gCGGGGATTTGCTCATCGTTTGCCAGTCAGGGGAACctgttg > 1:727635/1‑42 (MQ=19) gCGGGGATTTGCTCATCGTTTGCCAGTCAGGGGAACCTGTTgg > 1:3743939/1‑43 (MQ=19) aTTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTAt < 1:3097571/49‑1 (MQ=255) tGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCtt > 1:3189565/1‑71 (MQ=255) tGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCt < 1:1559815/70‑1 (MQ=255) cTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCtttt < 1:2351797/71‑1 (MQ=255) catcatTTGCCAGGCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGcc < 1:3132766/50‑1 (MQ=255) catTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTg > 1:2227638/1‑71 (MQ=255) atTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTgc > 1:2581569/1‑71 (MQ=255) | GATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGC > W3110S.gb/3285644‑3285774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |