| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,331,382 | G→T | 21.4% | R76R (CGC→CGA) | obgE ← | GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,331,382 | 0 | G | T | 21.4% | 66.9 / 6.4 | 28 | R76R (CGC→CGA) | obgE | GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base G (10/12); new base T (3/3); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
TGGTCGATTACACGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTT > W3110S.gb/3331314‑3331450 | tGGTCGATTACACGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTcgcg > 1:2330528/1‑71 (MQ=255) cacGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGcc < 1:1614150/70‑1 (MQ=255) gcgTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCgg > 1:3321100/1‑59 (MQ=255) gcgTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTc < 1:24210/71‑1 (MQ=255) cgTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCt < 1:3536940/71‑1 (MQ=255) taccGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCgggg > 1:2500037/1‑52 (MQ=255) accGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTcgcg > 1:1093936/1‑50 (MQ=255) ggCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTcgc > 1:745922/1‑46 (MQ=255) ggCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCgggg > 1:3848376/1‑48 (MQ=255) cACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTg < 1:1206169/71‑1 (MQ=255) cTTTAATCGTGACGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCa < 1:898852/71‑1 (MQ=255) cTTTAATCGTGACGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCa < 1:448761/71‑1 (MQ=255) cTTTAATCGTGACGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCa < 1:1728068/71‑1 (MQ=255) cTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTg < 1:1087841/58‑1 (MQ=255) tttAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCt > 1:3522468/1‑45 (MQ=255) ttAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTcgcg > 1:2109491/1‑41 (MQ=255) ttAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCgggt > 1:2488064/1‑42 (MQ=255) ttAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCgg > 1:1773734/1‑42 (MQ=255) ttAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGcg > 1:300189/1‑43 (MQ=255) tAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCt > 1:3325959/1‑43 (MQ=255) tCGTCACGTCTTTACCGCGCTTACCGGTACAGTcgcg > 1:20805/1‑37 (MQ=255) gTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTgc > 1:2618569/1‑41 (MQ=255) gTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTgc > 1:1810456/1‑41 (MQ=255) aCGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGAttt < 1:277639/71‑1 (MQ=255) tACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCaa < 1:620691/69‑1 (MQ=255) ccGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAAc < 1:2356464/70‑1 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGa < 1:2751402/71‑1 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGAGCTCTGCACGGAAAGAt < 1:1395431/59‑1 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTCGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGa < 1:2997427/71‑1 (MQ=255) gTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:1695475/1‑70 (MQ=255) gTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAg > 1:185073/1‑71 (MQ=255) gTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAg > 1:748071/1‑71 (MQ=255) tCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGa < 1:2477209/61‑1 (MQ=255) gcgGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGtt > 1:2826448/1‑69 (MQ=255) gcgGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGtt > 1:1768686/1‑69 (MQ=255) | TGGTCGATTACACGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTT > W3110S.gb/3331314‑3331450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |