| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,331,391 | G→A | 27.2% | G73G (GGC→GGT) | obgE ← | GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,331,391 | 0 | G | A | 27.2% | 30.4 / 8.9 | 22 | G73G (GGC→GGT) | obgE | GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base G (2/14); new base A (3/3); total (5/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
CACGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTC > W3110S.gb/3331324‑3331459 | cacGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGcc < 1:1614150/70‑1 (MQ=255) gcgTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTc < 1:24210/71‑1 (MQ=255) cgTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCt < 1:3536940/71‑1 (MQ=255) cACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTg < 1:1206169/71‑1 (MQ=255) cTTTAATCGTGACGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCa < 1:898852/71‑1 (MQ=255) cTTTAATCGTGACGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCa < 1:448761/71‑1 (MQ=255) cTTTAATCGTGACGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCa < 1:1728068/71‑1 (MQ=255) cTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTg < 1:1087841/58‑1 (MQ=255) aCGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGAttt < 1:277639/71‑1 (MQ=255) tACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCaa < 1:620691/69‑1 (MQ=255) ccGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAAc < 1:2356464/70‑1 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGa < 1:2751402/71‑1 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGAGCTCTGCACGGAAAGAt < 1:1395431/59‑1 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTCGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGa < 1:2997427/71‑1 (MQ=255) gTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:1695475/1‑70 (MQ=255) gTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAg > 1:185073/1‑71 (MQ=255) gTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAg > 1:748071/1‑71 (MQ=255) tCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGa < 1:2477209/61‑1 (MQ=255) gcgGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGtt > 1:2826448/1‑69 (MQ=255) gcgGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGtt > 1:1768686/1‑69 (MQ=255) ttGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATc < 1:197596/52‑1 (MQ=255) ttGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATc < 1:2661749/52‑1 (MQ=255) gcgcCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATa > 1:468427/1‑47 (MQ=255) gcgcCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTtctc > 1:3119331/1‑71 (MQ=255) cgcCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTtctc < 1:133859/70‑1 (MQ=255) | CACGCGTACCTACCGGCACTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTC > W3110S.gb/3331324‑3331459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |