| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,400,385 | A→G | 6.5% | S186S (TCT→TCC) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,400,385 | 0 | A | G | 6.5% | 166.3 / 2.7 | 61 | S186S (TCT→TCC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (27/30); new base G (2/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACC > W3110S.gb/3400317‑3400444 | agagAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACag < 1:778980/70‑1 (MQ=255)agagAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACag < 1:2324519/70‑1 (MQ=255)agagAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACag < 1:3769060/70‑1 (MQ=255)agagAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACag < 1:2918028/70‑1 (MQ=255)agagAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTAGAAACGGTCACCACCAATGCGCACag < 1:356117/70‑1 (MQ=255) gagaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa < 1:1055331/71‑1 (MQ=255) gagaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa < 1:1388948/71‑1 (MQ=255) gagaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa < 1:2750565/71‑1 (MQ=255) agaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACag > 1:2754919/1‑68 (MQ=255) gaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa > 1:2295291/1‑69 (MQ=255) gaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa > 1:700980/1‑69 (MQ=255) gaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa > 1:1706087/1‑69 (MQ=255) gaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaa > 1:3175564/1‑69 (MQ=255) aCCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAagag < 1:1145505/70‑1 (MQ=255) tAATTACGTCGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTa < 1:681361/71‑1 (MQ=255) tAATTACGACGCACATAGTTGGTGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTa < 1:2240528/71‑1 (MQ=255) tAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTa < 1:3811499/71‑1 (MQ=255) aaTTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTa < 1:2249604/70‑1 (MQ=255) aTTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTaa < 1:3845711/70‑1 (MQ=255) gCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCacac > 1:2416108/1‑69 (MQ=255) caTAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTc < 1:3184040/71‑1 (MQ=255) caTAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTc < 1:3748602/71‑1 (MQ=255) gatgatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAATCCACACCGTTCAAg > 1:235632/1‑67 (MQ=255) gatgatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACcaca > 1:1660073/1‑58 (MQ=255) gatgatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAg > 1:1632030/1‑67 (MQ=255) gatgatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAg > 1:2528259/1‑67 (MQ=255) gatgatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAg > 1:1235310/1‑67 (MQ=255) gatgatAGCTTCGTCGAAACGGTCAACACCAATGCGCACAGAAGAGGAGTAAACcaca > 1:1578757/1‑58 (MQ=255) aatgatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACcaca > 1:2351955/2‑58 (MQ=255) atgatAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGAt < 1:1047714/71‑1 (MQ=255) atAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATaa < 1:3417272/70‑1 (MQ=255) atAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATaa < 1:2685324/70‑1 (MQ=255) aGCTTCGTCGAAACGGTCACCACCAATGCGCACagaaga > 1:2161630/1‑39 (MQ=255) aGCTTCGTCGAAACGGTCACCACCAATGCGCACagaaga > 1:1381745/1‑39 (MQ=255) aGCTTCGTCGAAACGGTCACCACCAATGCGCACagaaga > 1:1535147/1‑39 (MQ=255) aGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAg > 1:1541922/1‑71 (MQ=255) aGCTTCGTCGAAACGGTCACCAACAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAg > 1:1600098/1‑61 (MQ=255) aGCTTAGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCaa > 1:3233377/1‑60 (MQ=255) gCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGc < 1:3250257/71‑1 (MQ=255) cTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGga < 1:166992/61‑1 (MQ=255) tcgtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATaa > 1:1506060/1‑64 (MQ=255) tcgtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATaa > 1:1984272/1‑64 (MQ=255) cgtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTaaa > 1:1569531/1‑42 (MQ=255) cgtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTaaa > 1:1792773/1‑42 (MQ=255) gtctAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACtt < 1:2285290/71‑1 (MQ=255) gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGt > 1:372682/1‑38 (MQ=255) gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGt > 1:3591270/1‑38 (MQ=255) gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACtt < 1:442043/71‑1 (MQ=255) gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACtt < 1:1547087/71‑1 (MQ=255) gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACtt < 1:3663666/71‑1 (MQ=255) gtcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACtt < 1:1893145/71‑1 (MQ=255) tcgAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTc < 1:526648/71‑1 (MQ=255) gAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTc < 1:2846724/69‑1 (MQ=255) aaaCGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTc < 1:3135176/48‑1 (MQ=255) aaaCGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTc < 1:3154212/48‑1 (MQ=255) aaaCGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGt < 1:798059/71‑1 (MQ=255) aCGGTCACCACCAATGCGCACGGAAGAGGAGTAAACCACGCCGTTCAAGGAGATAACAGCAACTTCAg > 1:1046731/1‑68 (MQ=255) aCGGTCACCACCAATGCGCACGGAAGAGGAGTAAACCACGCCGTTCAAGGAGATAACAGCAACTTCAg > 1:138800/1‑68 (MQ=255) cGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTgg > 1:2768360/1‑70 (MQ=255) gTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACa > 1:3560734/1‑55 (MQ=255) caccacCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacca > 1:3600277/1‑71 (MQ=255) caccaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTac < 1:298636/66‑1 (MQ=255) accaATGCGCACAGAAGAGGAGTAAACCACACCGTTCaa > 1:940372/1‑39 (MQ=255) accaATGCGCACAGAAGAGGAGTAAACCACACCGTTCaa > 1:1894539/1‑39 (MQ=255) accaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAAc < 1:1710088/48‑1 (MQ=255) accaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAAc < 1:2369749/48‑1 (MQ=255) accaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAAc < 1:1978737/48‑1 (MQ=255) ccaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTgg > 1:2672866/1‑61 (MQ=255) ccaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTgg > 1:2698983/1‑61 (MQ=255) ccaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccaccacc > 1:2039054/1‑71 (MQ=255) ccaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccaccacc < 1:2391604/71‑1 (MQ=255) ccaATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccaccacc < 1:2158993/71‑1 (MQ=255) caATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCaa > 1:237449/1‑51 (MQ=255) caATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCaa > 1:2890036/1‑51 (MQ=255) aTGCGCACGGAAGAGGAGTAAACCACGCCGTTCAAGGAGATa < 1:2834357/42‑1 (MQ=38) aTGCGCACGGAAGAGGAGTAAACCACGCCGTTCAAGGAGATa < 1:1720824/42‑1 (MQ=38) tGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGgag < 1:3654716/38‑1 (MQ=255) agaagaGGAGTAAACCACACCGTTCAAGGAGATAACAg > 1:3046775/1‑38 (MQ=255) agaagaGGAGTAAACCACACCGTTCAAGGAGATAACAg > 1:780887/1‑38 (MQ=255) agaagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAg < 1:3729598/47‑1 (MQ=255) | AGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACC > W3110S.gb/3400317‑3400444 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |